Development of Novel Spin-labeled Nucleosides which detect base-pairing
| Project/Area Number |
15590012
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Chemical pharmacy
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| Research Institution | KYUSHU UNIVERSITY |
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Principal Investigator |
ASO Mariko Kyushu University, Graduate School of Pharmaceutica ISciences, Research Associate, 大学院・薬学研究院, 助手 (30201891)
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| Co-Investigator(Kenkyū-buntansha) |
SUEMUNE Hiroshi Kyushu University, Graduate School of Pharmaceutica ISciences, Professor, 大学院・薬学研究院, 教授 (20095897)
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| Project Period (FY) |
2003 – 2004
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| Project Status |
Completed (Fiscal Year 2004)
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| Budget Amount *help |
¥3,600,000 (Direct Cost: ¥3,600,000)
Fiscal Year 2004: ¥1,400,000 (Direct Cost: ¥1,400,000)
Fiscal Year 2003: ¥2,200,000 (Direct Cost: ¥2,200,000)
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| Keywords | spin-labeled nucleoside / N-tert-butylaminoxyl / EPR / base pairing / hydrogen bonding / ESRスペクトル / pH依存症 / N-tert-アミノキシルラジカル / イノシン / アデノシン / シチジン / グアノシン |
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| Research Abstract |
We designed novel spin-labeled nucleosides, in which a spin source, an N-tert-butylaminoxyl radical, was directly attached to nucleobase. 2-N-tert-Butylaminoxyladenosine, 2-N-tert-butylaminoxylinosine, and 8-N-tert-butylaminoxylinosine, and 5-N-tert-butylaminoxylcytidine derivative were synthesized and thier stabilities and properties were studied by using electron peramagnetic resonance(EPR). It was of interest to investigate if EPR spectra of N-tert-butylaminoxylnucleosides changed upon formation of hydrogen bonding with complementary base. EPR spectra of O-silylated-5-N-tert-butylaminoxylcytidine in CH_2Cl_2 changed upon addition of O-silylated guanosine, which might form hydrogen bonds with the aminoxyl cytidine. 2-N-tert-Butylaminoxyladenosine was introduced into oligoncleotide and its EPR spectra were measured. The EPR spectrum of the obtained oligonucleotide showed three lines in phosphate buffer. The high line disappeared upon duplex formation with the complementary strand to show that the EPR spectra might give useful information on the structure of oligonucleotide. EPR spectra of 2-N-tert-butylaminoxylinosine showed pH dependency due to structural change of purine moiety
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Report
(3 results)
Research Products
(9 results)
