Glycome analysis by tandem two-dimensional chromatography
| Project/Area Number |
15590038
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Physical pharmacy
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| Research Institution | Chiba University |
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Principal Investigator |
TOYODA Hidenao Chiba University, Graduate School of Pharmaceutical Sciences, Associate Professor, 大学院・薬学研究院, 助教授 (70217579)
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| Project Period (FY) |
2003 – 2004
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| Project Status |
Completed (Fiscal Year 2004)
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| Budget Amount *help |
¥3,500,000 (Direct Cost: ¥3,500,000)
Fiscal Year 2004: ¥1,200,000 (Direct Cost: ¥1,200,000)
Fiscal Year 2003: ¥2,300,000 (Direct Cost: ¥2,300,000)
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| Keywords | Chromatography / Glycan / Glycosaminoglycan / Fluorescence / 高速液体クロマトグラフィー / ムチン型糖鎖 |
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| Research Abstract |
We have devised a method for glycome analysis by using on-line two-dimensional HPLC. N-linked oligosaccharides, mucins and glycosaminoglycans were separated simultaneously by the HPLC. We applied this method to a genetically tractable model organism, Drosophila melanogaster. In addition to this chemical analysis, western-blot analysis by use of lectin staining and immunostaining provides attractive tools to further understanding of basic physiological functions of glycoconjugates. Thus, we carried out glycoconjugate profiling of Drosophila by 1D or 2D-electrophoresis with western-blot analysis followed lectin staining and immunostaining. Drosophila glycoconjugates were well characterized by SBA, ConA and WGA lectins and the method was applied to flies bearing mutations in glyco-genes. Furthermore, proteoglycans were profiled by western-blot analysis with immunostaining. These methods are planning to be employed in the proteo-glycomics study.
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Report
(3 results)
Research Products
(10 results)
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[Journal Article] Molecular cloning and identification of 3'-phosphoadenosine 5'-phosphosulfate transporter2003
Author(s)
S.Kamiyama, T.Suda, R.Ueda, M.Suzuki, R.Okubo, N.Kikuchi, Y.Chiba, S.Goto, H.Toyoda, K.Saigo, M.Watanabe, H.Narimatsu, Y.Jigami, S.Nishihara
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Journal Title
Journal of Biological Chemistry 278
Pages: 25958-25963
Description
「研究成果報告書概要(和文)」より
Related Report
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[Journal Article] Cellular binding of hepatitis C virus envelope glycoprotein E2 requires cell surface heparan sulfate2003
Author(s)
H.Barth, C.Shafer, M.I.Adah, F.Zhang, R.J.Linhardt, H.Toyoda, A.Kinoshita-Toyoda, T.Toida, T.H.Van Kuppevelt, E.Depla, F.Von Weizsacker, H.E.Blum, T.F.Baumert
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Journal Title
Journal of Biological Chemistry 278
Pages: 41003-41012
Description
「研究成果報告書概要(和文)」より
Related Report
-
-
[Journal Article] Cellular binding of hepatitis C virus envelope glycoprotein E2 requires cell surface heparan sulfate2003
Author(s)
H.Barth, C.Schafer, M.I.Adah, F.Zhang, R.J.Linhardt, H.Toyoda, A.Kinoshita-Toyoda, T.Toida, T.H.Van Kuppevelt, E.Depla, B.F.Von Weizsacker, H.E.Blum, T.F.Baumert
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Journal Title
Journal of Biological Chemistry 278
Pages: 41003-41012
Description
「研究成果報告書概要(欧文)」より
Related Report
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[Publications] S.Kamiyama, T.Suda, R.Ueda, M.Suzuki, R.Okubo, N.Kikuchi, Y.Chiba, S.Goto, H.Toyoda, K.Saigo, M.Watanabe, H.Narimatsu, Y.Jigami, S.Nishihara: "Molecular cloning and identification of 3´-phosphoadenosine 5´-phosphosulfate transporter"Journal of Biological Chemistry. 278. 25958-25963 (2003)
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[Publications] H.Barth, C.Schafer, M.I.Adah, F.Zhang, R.J.Linhardt, H.Toyoda, A.Kinoshita-Toyoda, T.Toida, T.H.Van Kuppevelt, E.Depla, F.Von Weizsacker, H.E.Blum, T.F.Baumert: "Cellular binding of hepatitis C virus envelope glycoprotein E2 requires cell surface heparan sulfate"Journal of Biological Chemistry. 278. 41003-41012 (2003)
