| Project/Area Number |
15590044
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Physical pharmacy
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| Research Institution | Kyushu University |
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Principal Investigator |
NAKAZONO Manabu Kyushu University, Graduate school of pharmaceutical sciences, 大学院・薬学研究院, 助手 (90336074)
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| Co-Investigator(Kenkyū-buntansha) |
ZAITSU Kiyoshi Kyushu University, Graduate school of pharmaceutical sciences, 大学院・薬学研究院, 教授 (70091329)
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| Project Period (FY) |
2003 – 2004
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| Project Status |
Completed (Fiscal Year 2004)
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| Budget Amount *help |
¥3,600,000 (Direct Cost: ¥3,600,000)
Fiscal Year 2004: ¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 2003: ¥2,600,000 (Direct Cost: ¥2,600,000)
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| Keywords | Polyphenol / Chemiluminescence / Quaternary ammonium / Dendrimer / Fluorescence |
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| Research Abstract |
1-Etyl-3-(3-dimethylaminopropyl)carbodiimide and cetyltrimethylammonium bromide gave an enhancement on chemiluminescence development of polyphenol dendrimer (Luminescence,18,239-242,2003).
We estimated the CH-π interaction between polyphenol dendrimer and acetylcholine bromide (Ach) using ^1H-NMR to develop chemiluminecent method based on noncovalent bond. The N-methyl group of Ach shifted to the high magnetic field in the range of 0.02-0.08 ppm. The complex concentration reached maximum in the molar fraction 0.3 from Job's plots analysis. This indicated that a few of complexes formed. From the effect of Ach on the chemiluminescence of polyphenol dendrimer, 50 mM Ach gave 2.4-fold chemiluminescence enhancement.
To synthesize fluorescent dendrimers, the first generation fluorescent dendrimers which have 2-Hydroxyquinoxaline(HQ),4-Hydroxycoumarin(HC) and 9-(Hydroxymethyl)anthracene(HA) as core molecules were synthesized. 3,4,5-Tribenzyloxybenzoyl group gave 1.5- and 1.3-fold enhancement on the fluorescence of HQ and HA, respectively. 3,4,5-Trihydroxybenzoyl group gave 3.1-fold enhancement on the fluorescence of HQ.
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