| Project/Area Number |
15590049
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Physical pharmacy
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| Research Institution | Kyoritsu University of Pharmacy |
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Principal Investigator |
KANAZAWA Hideko Kyoritsu College of Pharmacy, Pharmaceutical Science, Professor, 薬学部, 教授 (10240996)
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| Project Period (FY) |
2003 – 2004
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| Project Status |
Completed (Fiscal Year 2004)
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| Budget Amount *help |
¥3,500,000 (Direct Cost: ¥3,500,000)
Fiscal Year 2004: ¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 2003: ¥2,500,000 (Direct Cost: ¥2,500,000)
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| Keywords | poly (N-isopropylacrylamide) / temperature-responsive chromatography / functional polymer / functional surface / HPLC / packing material / environmentally responsive / ポリ(N-イソプロピルアクリルアミド |
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| Research Abstract |
We report development of a novel analytical HPLC technique of nucleotides with a constant aqueous mobile phase. In this study, we have designed a new pH/temperature-responsive copolymer of N-isopropylacrylamide (NIPAAm), butyl methacrylate (BMA) and N,N-dimethylaminopropylacrylamide (DMAPAAm). The copolymer was grafted on aminopropyl silica beads and the products were evaluated as HPLC packing materials for an ion-exchange temperature responsive chromatography. The property of the surface of the stationary phase altered from hydrophilic to hydrophobic and from charged to non-charged by changes in temperature and pH. In addition, it is possible that appears and hides ion-exchange groups on the polymer chain surface by the temperature changes. Nucleotides interactions with this stationary phase were controlled by the temperature and the pH with constant aqueous mobile phase. PH-/temperature responsive chromatography is expected to be useful for separation of pharmaceuticals and biomolecules as well as nucleotides. We believe that the proposed temperature-responsive chromatography is likely to be useful for diverse applications in biotechnology including protein purification and bioseparations.
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