Function and distribution of vanilloid receptor TRPVlas non-selective cation channels
| Project/Area Number |
15590056
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Biological pharmacy
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| Research Institution | Chiba University |
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Principal Investigator |
HARIE Syunji Chiba University, Graduate School of Pharmaceutical Sciences, Department of Chemical Pharmacology, Associate Professor, 大学院・薬学研究院, 助教授 (50209285)
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| Project Period (FY) |
2003 – 2004
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| Project Status |
Completed (Fiscal Year 2004)
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| Budget Amount *help |
¥3,600,000 (Direct Cost: ¥3,600,000)
Fiscal Year 2004: ¥1,400,000 (Direct Cost: ¥1,400,000)
Fiscal Year 2003: ¥2,200,000 (Direct Cost: ¥2,200,000)
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| Keywords | TRPV1 / Gastrointestinal tract / Capsaicin / Immunohistochemistry / Calcium channel blocker / Gastroprotection / Sino-Japanese medicine / Sensoy nerve / VR1 / 胃 / 気管支 / 血管内皮細胞 / アラキドン酸 / カルシウム流入 |
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| Research Abstract |
It is well known that some physiological functions are elicited via non-Selective cation channels. The function and the distribution of vanilloid receptor TRPV1(transient receptor potential vanillbid-1) in rodent were studied in order to clarify whether TRPV1 functions as non-selective cation channels. As a result of this project, I got the following results.
(1)Peripheral neuron cell : Capsaicin, an agonist of TRPV1, induced transmitter release from PC12 cells, a rat pheochromocytoma cell line, through TRPV1.
(2)Gastric function : Capsaicin protects gastric mucosa through activation of TRPV1 in rat gastric corpus lesion and antral ulcer models. In addition, it induced gastric acid secretion through central TRPV1 in anesthetized rats.
(3)Distribution of TRPV1 in stomach : Numerous TRPV1-immunoreactive axons were also found around arterioles in the submucosal layer. A large number of nerve fibers were seen in the smooth muscle layer and in the myenteric nerve plexus.
(4)Exploration of natural occurring product acting on Ca^<2+> channel blocker-resistant Ca^<2+> influx : Mesaconitine, a major constituent of aconite, acts on non-selective cation channel, leading to release of nitric oxide from the endothelium in isolated rat aorta. Gingerol, a major constituent of ginger, acts on non-selective cation channel, leading to enhancement of peristaltic movement of intestinal tract.
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Report
(3 results)
Research Products
(40 results)
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[Journal Article] Indole alkaloids of a Thai medicinal herb, Mitrogyna speciosa, that has opioid agonistic effect in guinea-pig ileim.2005
Author(s)
Horie, S., Koyama, F., Takayama, H., Ishikawa, H., Aimi, N., Ponglux, P., Matsumoto, K., Murayama, T.
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Journal Title
Planta Med. 71(3)
Pages: 231-236
Description
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[Journal Article] Decrease in cytosolic phospholipase A_<2□> mRNA levels by reactive oxygen species via MAP Kinase pathways in PC12 cells : effects of dopaminergic neurotoxns.2005
Author(s)
Akiyama, N., Shimma, N., Takashiro, Y., Hatori, Y., Hirabayashi, T., Horie, S., Saito T., Murayama, T.
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Journal Title
Cell Signal. 17(5)
Pages: 597-604
Description
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[Journal Article] Antinociception, tolerance and withdrawal symptoms induced by 7-hydroxymitragynine, an alkaloid from the Thai medicinal herb Mitragyna speciosa.2005
Author(s)
Matsumoto, K., Horie, S., Takayama, H., Ishikawa, H., Aimi, N., Ponglux, D., Murayama, T., Watanabe, K.
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Journal Title
Description
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[Journal Article] Protective role of vanilloid receptor type 1 in HCl-induced gastric mucosal lesions in rats.2004
Author(s)
Horie, S., Yamamoto, H., Michael, G.J., Uchida, M., Belai, A., Watanabe, K., Priestley, J.V., Murayama, T.
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Journal Title
Scand.J.Gastroenterol. 39(4)
Pages: 303-312
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Mesaconitine-jnduced relaxation in rat aorta : role of Na^+/Ca^<2+> exchangers in endothelial cells.2004
Author(s)
Ogura, J., Mitamura, M., Someya, A., Shimamura, K, Takayama, H., Aimi, N., Horie, S., Murayama, T.
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Journal Title
Eur.J.Pharmacol. 483(2-3)
Pages: 139-146
Description
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[Journal Article] Contraction of isolated guinea-pig ileum by urotensin II via activation of ganglionic cholinergic neurons and acetylcholine release.2003
Author(s)
Horie, S., Yasuda S., Tsurumaki Y., Someya A., Saito T., Okuma Y., Nomura Y., Hirabayashi T., Murayama, T.
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Journal Title
Neuropharmacology 45(7)
Pages: 1019-1027
Description
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[Journal Article] Involvement of glutamate and □-amino-butyric acid receptor systems on gastric acid secretion induced by activation of □-opioid receptors in the central nervous system in rats.2003
Author(s)
Minowa, S., Ishihara, S., Tsuchiya, S., Horie, S., Watanabe, K, Murayama, T.
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Journal Title
Br.J.Pharmacol. 138(6)
Pages: 1049-1058
Description
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