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Physiological action of nitric oxide produced by a stimulation of endotoxin from Helicobacter pylori on gastric mucosa

Research Project

Project/Area Number 15590087
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Biological pharmacy
Research InstitutionYamagata Promotional Organization for Industrial Technology (2004)
Yamagata Public Corporation For the Development Of Industry, Institute Life Support Technology (2003)

Principal Investigator

YOSHIMURA Tetsuhiko  Yamagata Promotional Organization for Industrial Technology, Institute for Life Support Technology, Deputy Director General, 生物ラジカル研究所, 副所長 (70271517)

Co-Investigator(Kenkyū-buntansha) KASAI Shigenobu  Yamagata Promotional Organization for Industrial Technology, Institute for Life Support Technology, Deputy Director General, 生物ラジカル研究所, 研究員 (70342730)
MORITA Hidetoshi  Azabu University, School of Veterinary Medicine, Associate Professor, 獣医学部, 助教授 (70257294)
Project Period (FY) 2003 – 2004
Project Status Completed (Fiscal Year 2004)
Budget Amount *help
¥3,600,000 (Direct Cost: ¥3,600,000)
Fiscal Year 2004: ¥1,400,000 (Direct Cost: ¥1,400,000)
Fiscal Year 2003: ¥2,200,000 (Direct Cost: ¥2,200,000)
KeywordsHelicobacter pylori / endotoxin / nitric oxide / gastric mucosa / inducible nitric oxide synthase / cyclooxygenase / inflammatory cytokine / 誘導型NO合成酵素(iNOS) / シクロオキシゲナーゼ(COX)
Research Abstract

Helicobacter pylori, a gram-negative bacterium, is the primary cause of gastritis and a major contributor to peptic ulcer disease. Despite its importance as a human pathogen, principal factor of pathogenicity of the bacterium remains to be identified Nitric oxide (NO) released due to the induction of Ca^<2+> independent isoform of nitric oxide synthase (iNOS) by lipopolysaocharide or endotoxin from a gram-negative bacterium bas been known to be deleterious to a gastric mucosa. In the present shady, to elucidate the physiological action of H.pylori LPS on gastric mucosa and the role of NO, we were carried out both in vivo and in vitro study by employing E.coli LPS as a control.
1. IT pylori LPS was isolated from gram-order bacteria by the phenol-water extraction method, and then further purified by tine enzymatic digestion of RNA, DNA, and proteins.
2. In vitro cross talk between products from iNOS and cyclooxygenase (COX) in the rat gastric mucosa during endotoxemia was examined in vivo. The results demonstrate that in the E cob LPS-treated rat gastric mucosa, PGE2, a COX product, enhances after activation of iNOS. The effect of COX activity on iNOS-NO pathway is important in the regulation of gastric mucosal integrity inflammatory states.
3. We further elucidated the interaction of LPS with gastric epithelial cells by using a normal mouse gastric surface mucous cell line (GSM06). Our findings suggest that both LPSs from H.pylori and E.coli induce the iNOS and Toll-like receptor (TLR) 2 through the TLR 4 expressed constitutively in GSM06. This mechanism on TLR 2 induction can be crucial for maintenance of gastric mucosal integrity.

Report

(3 results)
  • 2004 Annual Research Report   Final Research Report Summary
  • 2003 Annual Research Report
  • Research Products

    (19 results)

All 2004 2003 Other

All Journal Article (15 results) Book (1 results) Publications (3 results)

  • [Journal Article] In Vivo Study on Cross-Talk between Inducible Nitric Oxide Synthase and C yclooxygenase in Rat Gastric Mucosa : Effect of Prostaglandin E2 on NO Pro2004

    • Author(s)
      K.Uno
    • Journal Title

      J. Pharmacol. Exp. Ther 309

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Spin Trapping of Nitric Oxide with the Iron-Dithiocarbamate Complex : Chemistry and Biology2004

    • Author(s)
      T.Yoshimura
    • Journal Title

      Antioxid Redox Signal. 6

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Spinnokinetic analyses of blood disposition and biliary excretion of nitric oxide (NO)-Fe(II)-N-(Dithiocarboxy)sarcosine complex in rats : BCM-ESR and BEM-ESR studies2004

    • Author(s)
      H.Yasui
    • Journal Title

      Free Radic Res. 38

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Anti-microbial action against verotoxigenic Escherichia coli O157 : H7 by nitric oxide derived from sodium nitrite2004

    • Author(s)
      H.Morita
    • Journal Title

      Biosci. Biotech. Biochem. 68

    • NAID

      10013144505

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Annual Research Report 2004 Final Research Report Summary
  • [Journal Article] In Vivo Study on Cross-Talk between Inducible Nitric Oxide Synthase and Cyclooxygenase in Rat Gastric Mucosa : Effect of Prostaglandin E2 on NO Production2004

    • Author(s)
      K.Uno
    • Journal Title

      J.Pharmacol.Exp.Ther., 309

      Pages: 995-1002

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Spin Trapping of Nitric Oxide with the Iron-Dithiocarbamate Complex : Chemistry and Biology2004

    • Author(s)
      T.Yoshimura
    • Journal Title

      Antioxid.Redox Signal., 6

      Pages: 639-647

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Spinnokinetic analyses of blood disposition and biliary excretion of nitric oxide (NO)-Fe(II)-N-(Dithiocarboxy)sarcosine complex in rats : BCM-ESR and BEM-ESR studies2004

    • Author(s)
      H.Yasui
    • Journal Title

      Free Radic Res 38

      Pages: 1061-1072

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Anti-microbial action against verotoxigenic Escherichia coli O157:H7 by nitric oxide derived from sodium nitrite2004

    • Author(s)
      H.Morita
    • Journal Title

      Biosci.Biotech.Biochem., 68

      Pages: 1027-1034

    • NAID

      10013144505

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] In Vivo Study on Cross-Talk between Inducible Nitric Oxide Synthase and Cyclooxygenase in Rat Gastric Mucosa : Effect of Prostaglandin E2 on NO Pro2004

    • Author(s)
      K.Uno
    • Journal Title

      J. Pharmacol. Exp. Ther 309

      Pages: 995-1002

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Spin Trapping of Nitric Oxide with the Iron-Dithiocarbamate Complex : Chemistry and Biology2004

    • Author(s)
      T.Yoshimura
    • Journal Title

      Antioxid. Redox Signal. 6

      Pages: 639-647

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Spinnokinetic analyses of blood disposition and biliary excretion of nitric oxide (NO)-Fe(II)-N-(Dithiocarboxy) sarosine complex in rats : BCM-ESR and BEM-ESR studies2004

    • Author(s)
      H.Yasui
    • Journal Title

      Free Radic Res. 38

      Pages: 1061-1072

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Direct detection of nitric oxide and its role in maintaining gastric mucosal integrity following ethanol-induced injury in rats2003

    • Author(s)
      H.Sugata
    • Journal Title

      Free Radic. Res. 37

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Early changes in alveolar fluid clearance by nitric oxide in endotoxin-induced pulmonary inflammation in rats2003

    • Author(s)
      H.Tsuboch
    • Journal Title

      Am. J. Respir. Crit. Care Med. 167

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Direct detection of nitric oxide and its role in maintaining gastric mucosal integrity following ethanol-induced injury in rats2003

    • Author(s)
      H.Sugata
    • Journal Title

      Free Radic.Res., 37

      Pages: 159-169

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Early changes in alveolar fluid clearance by nitric oxide in endotoxin-induced pulmonary inflammation in rats2003

    • Author(s)
      H.Tsuboch
    • Journal Title

      Am.J.Respir.Crit.Care Med., 167

      Pages: 205-210

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Book] 電子スピンサイエンス&スピンテクノロジー入門、6.2 バイオ電子スピンサイエンス2003

    • Author(s)
      吉村哲彦
    • Total Pages
      10
    • Publisher
      電子スピンサイエンス学会監修
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Publications] H.Sugata: "Direct detection of nitric oxide and its role in mainitaining gastric mucosal intrgrity following ethanol-induced injury in rats"Free Radic.Res. 37. 159-169 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] H.Tsubochi: "Early changes in alveolar fluid clearance by nitric oxide in endotoxin-induced pulmonary inflammation in rats"Am.J.Respir.Crit.Care Med. 167. 205-210 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] 吉村哲彦: "電子スピンサイエンス&スピンテクノロジー入門、6.2バイオ電子スピンサイエンス"電子スピンサイエンス学会監修. 10 (2003)

    • Related Report
      2003 Annual Research Report

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Published: 2003-04-01   Modified: 2016-04-21  

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