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Intestinal absorption of dietary compounds and quantitative analysis of their influences on drug absorption

Research Project

Project/Area Number 15590141
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Medical pharmacy
Research InstitutionTokyo University of Pharmacy and Life Sciences

Principal Investigator

MIZUMA Takashi  Tokyo University of Pharmacy and Life Sciences, School of Pharmacy, Associate Professor, 薬学部, 助教授 (80229715)

Co-Investigator(Kenkyū-buntansha) HAYASHI Masahiro  Tokyo University of Pharmacy and Life Sciences, School of Pharmacy, Professor, 薬学部, 教授 (20012669)
Project Period (FY) 2003 – 2005
Project Status Completed (Fiscal Year 2005)
Budget Amount *help
¥3,300,000 (Direct Cost: ¥3,300,000)
Fiscal Year 2005: ¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 2004: ¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 2003: ¥2,300,000 (Direct Cost: ¥2,300,000)
Keywordsintestinal availability / bioavailability / drug-drug interactions / drug-nutrient interactions / intestinal metabolism / sulfate conjugation metabolism / Caco-2 cells / 抱合代謝 / 腸管吸収 / カテキン / 相互作用 / 飲食物 / Caco-2 / 培養細胞 / 食物成分 / 薬物速度論
Research Abstract

Our research group has studied on intestinal absorption of benzyl β-glucoside contained in Prunus mume according to the everted intestine and Ussing type chamber methods using rat small intestines. Both types of experiments showed that benzyl β-glucoside was absorbed and in part metabolized during absorption, and that β-glucoside was transported in part by Na^+/glucose cotransporter (SGLT1). Moreover, it was obtained a novel finding that the SGLT1-mediated transport increased the intestinal availability of benzyl β-glucoside. Glucuronidation and sulfation metabolism were studied in Caco-2 cells with α-naphthol, a model drug. Both metabolic activities could be detected in Caco-2 cell monolayers. Moreover, both metabolic activities were also found in Caco-2 cell suspensions. Sulfation metabolism of α-naphthol in cell monolayers was inhibited by isoproterenol or terbutaline (substrates for intestinal sulfotransferase), whereas transport of α-naphthol was increased. This shows that we could establish an in vitro system to detect both conjugative metabolism and absorption (transport) using Caco-2 cell monolayers and suspensions. Thereby, influences of dietary compounds on intestinal metabolism and absorption were assessed using this in vitro detection system. Sulfation metabolism of α-naphthol in cell suspensions was significantly inhibited by the presence of (-)-epigallocatechin gallate (EGCG), (±)-catechin and tannic acid. Glucuronidation metabolism was inhibited by the presence of (-)-EGCG. Sulfation and glucuronidation metabolism of α-naphthol were inhibited by the presence of (-)-EGCG, (±)-catechin and tannic acid. In contrast, the transport of α-naphthol across Caco-2 cell monolayers was enhanced. Thus, our study supported by a Grant-in-Aid for Scientific Research (C) from the Japan Society for the Promotion of Science showed drug-nutrients (dietary compounds) interactions in drug absorption raised by intestinal conjugative metabolism.

Report

(4 results)
  • 2005 Annual Research Report   Final Research Report Summary
  • 2004 Annual Research Report
  • 2003 Annual Research Report
  • Research Products

    (24 results)

All 2006 2005 2004

All Journal Article (21 results) Book (3 results)

  • [Journal Article] 糖トランスポーターを利用したDDS2006

    • Author(s)
      水間 俊
    • Journal Title

      Drug Delivery System 21

      Pages: 126-133

    • NAID

      10018118893

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Differentiation of organ availability by sequential analysis and simultaneous analysis : intestinal conjugative metabolism impacts on intestinal availability in humans2005

    • Author(s)
      Takashi Mizuma, Kosuke Kawashima, Shuichi Sakai, Sayaka Sakaguchi, Masahiro Hayashi
    • Journal Title

      1. Pharm. Sci. 94

      Pages: 571-575

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Intestinal SGLTI-mediated absorption and metabolism on benzyl β-glucoside contained in Purunus mume : carrier- mediated transport increases intestinal availability.2005

    • Author(s)
      Takashi Mizuma, Maya Nakamura, Hiroji Ina, oshio Miyazaki, Masahiro Hayashi.
    • Journal Title

      Aiochim. Biophys. Acta. 1722

      Pages: 218-223

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Simultaneous HPLC assay of lenampicillin (ampicillin prodrug), ampicillin, and marker compound for high - throughput in vitro assessment of intestinal absorption in vitro assessment of intestinal absorption2005

    • Author(s)
      Takashi Mizuma, Sayaka Sakaguchi, Masahiro Hayashi
    • Journal Title

      J. Liq. Chromtogr. Rel. Technol. 28

      Pages: 1531-1537

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Kinetic characterization of glycosidase activity from disaccharide conjugate to monosaccharide conjugate in Caco-2 cells2005

    • Author(s)
      Takashi Mizuma, Norihiko Fuseda, Masahiro Hayashi
    • Journal Title

      J. Pharm. Pharmacol 57

      Pages: 661-664

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Differentiation of organ availability by sequential analysis and simultaneous analysis : intestinal conjugative metabolism impacts on intestinal availability in humans2005

    • Author(s)
      Takashi Mizuma, Kosuke Kawashima, Shuichi Sakai, Sayaka Sakaguchi, Masahiro Hayashi
    • Journal Title

      J.Pharm.Sci. 94

      Pages: 571-575

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Intestinal SGLT1-mediated absorption and metabolism of benzyl β-glucoside contained in Purunus mume : carrier-mediated transport increases intestinal availability.2005

    • Author(s)
      Takashi Mizuma, Maya Nakamura, Hiroji Ina, Toshio Miyazaki, Masahiro Hayashi.
    • Journal Title

      Biochim.Biophys.Acta. 1722

      Pages: 218-223

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Simultaneous HPLC assay of lenampicillin (ampicillin prodrug), ampicillin, and marker compound for high-throughput in vitro assessment of intestinal absorption and metabolism using Caco-2 cells.2005

    • Author(s)
      Takashi Mizuma, Sayaka.Sakaguchi, Masahiro Hayashi.
    • Journal Title

      J.Liq.Chromtogr.Rel.Technol. 28

      Pages: 1531-1537

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Kinetic characterization of glycosidase activity from disaccharide conjugate to monosaccharide conjugate in Caco-2 cells.2005

    • Author(s)
      Takashi Mizuma, Norihiko Fuseda, Masahiro Hayashi.
    • Journal Title

      J.Pharm.Pharmacol. 57

      Pages: 661-664

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] HIGH PERFORMANCE LIQUID CHROMATOGRAPHIC SIMULTANEOUS ASSAY OF LENAMPICILLIN (AMPICILLIN PRODRUG), AMPICILLIN AND MARKER COMPOUND FOR HIGH-THROUGHPUT IN VITRO ASSESSMENT OF INTESTINAL ABSORPTION AND METABOLISM USING CACO-2 CELLS2005

    • Author(s)
      Mizuma, T., Sakaguchi, S.et al.
    • Journal Title

      J.Liquid Chromatography & Related Technologies 28

      Pages: 1531-1537

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Intestinal SGLT1-mediated absorption and metabolism of benzyl beta-glucoside contained in Prunus mume.2005

    • Author(s)
      Mizuma T, Nakamura M, Ina H, Miyazaki T, Hayashi M.
    • Journal Title

      Biochim Biophys Acta. 1722

      Pages: 218-223

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Differentiation of organ availability by sequential and simultaneous analyses : Intestinal conjugative metabolism impacts on intestinal availability in humans.2005

    • Author(s)
      Mizuma T, Kawashima K, Sakai S, Sakaguchi S, Hayashi M.
    • Journal Title

      J Pharm Sci 94(3)

      Pages: 571-575

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Dietary polyphenols (-)-epicatcchin and chrysin inhibit intestinal glucuronidation metabolism to increase drug absorption2004

    • Author(s)
      Takashi Mizuma, Shoji Awazu
    • Journal Title

      J. Pharm. Sci. 93

      Pages: 2407-2410

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Factors affecting glucuronidation activity in Caco - 2 cells.2004

    • Author(s)
      Takashi Mizuma, Ryoichi Momota, Makoto Haga, Masahiro Hayashi
    • Journal Title

      Drug Metab Pharmacokinet. 19

      Pages: 130-134

    • NAID

      10012923619

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Does the well-stirred model assess the intestinal first -pass effect well2004

    • Author(s)
      Takashi Mizuma, Akira Tsuji, Masahiro Hayashi
    • Journal Title

      J Pharm Pharmacol. 56

      Pages: 1579-1599

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Dietary polyphenols (-)-epicatechin and chrysin inhibit intestinal glucuronidation metabolism to increase drug absorption.2004

    • Author(s)
      Takashi Mizuma, Shoji Awazu
    • Journal Title

      J.Pharm.Sci. 93

      Pages: 2407-2410

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Factors affecting glucuronidation activity in Caco-2 cells.2004

    • Author(s)
      Takashi Mizuma, Ryoichi Momota, Makoto Haga, Masahiro Hayashi.
    • Journal Title

      Drug Metab Pharmacokinet. 19

      Pages: 130-134

    • NAID

      10012923619

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Does the well-stirred model assess the intestinal first-pass effect well?2004

    • Author(s)
      Takashi Mizuma, Akira Tsuji, Masahiro Hayashi.
    • Journal Title

      J Pharm Pharmacol. 56

      Pages: 1597-1599

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Factors affecting glucuronidation activity in Caco-2 cells.2004

    • Author(s)
      Mizuma T, Momota R, Haga M, Hayashi M.
    • Journal Title

      Drug Metab Pharmacokinet. 19(2)

      Pages: 130-134

    • NAID

      10012923619

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Dietary polyphenols (-)-epicatechin and chrysin inhibit intestinal glucuronidation metabolism to increase drug absorption2004

    • Author(s)
      Mizuma T, Awazu S.
    • Journal Title

      J Pharm Sci. 93(9)

      Pages: 2407-2410

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Does the well-stirred model assess the intestinal first-pass effect well?2004

    • Author(s)
      Mizuma T, Tsuji A, Hayashi M.
    • Journal Title

      J Pharm Pharmacol. 56(12)

      Pages: 1597-1599

    • Related Report
      2004 Annual Research Report
  • [Book] 創薬方法論 : ソリューションガイド20052006

    • Author(s)
      水間 俊
    • Publisher
      情報計算化学生物学会
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Book] 生物薬剤学2006

    • Author(s)
      水間 俊
    • Publisher
      南江堂
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Book] 創薬方法論 : ソリューションガイド20052005

    • Author(s)
      水間 俊
    • Total Pages
      64
    • Publisher
      情報計算化学生物学会
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary

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Published: 2003-04-01   Modified: 2016-04-21  

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