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Investigation of interstitial cells of Cajal and pacemaker mechanism of the gastrointestinal tract using new culture system

Research Project

Project/Area Number 15590158
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field General anatomy (including Histology/Embryology)
Research InstitutionNagoya University

Principal Investigator

TORIHASHI Shigeko  Nagoya University, Graduate School of Medicine, Associate Professor, 大学院・医学系研究科, 助教授 (90112961)

Co-Investigator(Kenkyū-buntansha) NAKAYAMA Shinsuke  Nagoya University, Graduate School of Medicine, Associate Professor, 大学院・医学系研究科, 助教授 (30192230)
Project Period (FY) 2003 – 2004
Project Status Completed (Fiscal Year 2004)
Budget Amount *help
¥3,600,000 (Direct Cost: ¥3,600,000)
Fiscal Year 2004: ¥1,400,000 (Direct Cost: ¥1,400,000)
Fiscal Year 2003: ¥2,200,000 (Direct Cost: ¥2,200,000)
KeywordsPacemaker cell / Interstitial cells of Cajal / gastrointestinal tract / mouse ES cell / cell culture / development / BMP / 消化管運動 / ペースメーカー / 自動運動 / ES細胞 / PDGF / in situ hybridization / c-Kit / マウス胚性幹細胞(ES細胞) / 転写因子
Research Abstract

In the period of 2003
We advanced the culture system of cell clusters isolated from the muscle layer of the mouse small intestine and confirmed that the cell cluster kept its original characteristic features in vitro. Using this method we isolated interstitial cells of Cajal(ICC) and immunize to rats to make a monoclonal antibody. We established the clone to produce antibody(AIC). This antibody is suitable for morphological studies because immunoreactivity was stable for paraformaldehyde fixation, and we applied for a patent. We plan to identify the epitope binding to AIC. Dr.Nakayama also advanced the study of pacemaker mechanism using this culture system.
In the period of 2004
The other culture system using mouse ES cells was studied and analyzed the formation of gut-like structures. We showed that formation process of gut-like structures mimicked the gut organogenesis, and several transcription factors crucial for the development of endoderm and gut organogenesis were expressed as a similar manner to mouse embryo. We plan to study differentiation of smooth muscles and ICC using this culture system.

Report

(3 results)
  • 2004 Annual Research Report   Final Research Report Summary
  • 2003 Annual Research Report
  • Research Products

    (38 results)

All 2005 2004 2003 Other

All Journal Article (31 results) Book (4 results) Patent(Industrial Property Rights) (1 results) Publications (2 results)

  • [Journal Article] Involvement of ryanodine receptors in muscarinic receptor-mediated membrane current oscillation in urinary bladder smooth muscle.2005

    • Author(s)
      Kajioka S
    • Journal Title

      Am J Physiol Cell Physiol. 288

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Purinergic modulation of pacemaker Ca^<2+> activity in interstitial cells of Cajal.2005

    • Author(s)
      Furuzono S
    • Journal Title

      Neuropharmacology. 48

      Pages: 264-273

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Co-contribution of IP3R and Ca^<2+> Influx Pathways to Pacemaker Ca^<2+> Activity in Stomach ICC.2005

    • Author(s)
      Liu HN
    • Journal Title

      J Biol Rhythms 20

      Pages: 15-16

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Involvement of ryanodine receptors in muscarinic receptor-mediated membrane current oscillation in urinary bladder smooth muscle.2005

    • Author(s)
      Kajioka S. et al.
    • Journal Title

      Am J Physiol Cell Physiol. 288

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Purinergic modulation of pacemaker Ca(2+) activity in interstitial cells of Cajal.2005

    • Author(s)
      Furuzono S. et al.
    • Journal Title

      Neuropharmacology 48

      Pages: 264-273

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Co-contribution of IP3R and Ca^<2+> Influx Pathways to Pacemaker Ca^<2+> Activity in Stomach ICC.2005

    • Author(s)
      Liu HN.et al.
    • Journal Title

      J Biol Rhythms. 20

      Pages: 16-16

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Involvement of ryanodine receptors in muscarinic receptor-mediated membrane current oscillation in urinary bladder smooth muscle.2005

    • Author(s)
      Kajioka
    • Journal Title

      Am J Physiol Cell Physiol 288

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Isolation and characterization of resident macrophages from the smooth muscle layers of murine small intestine.2004

    • Author(s)
      Ozaki H
    • Journal Title

      Neurogastroenterology and Motility 16

      Pages: 39-51

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Annual Research Report 2004 Final Research Report Summary
  • [Journal Article] In vitro organogenesis of gut-like structures from mouse embryonic stem cells.2004

    • Author(s)
      Kuwahara M
    • Journal Title

      Neurogastroenterology and Motility 16 Suppl

      Pages: 14-16

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Annual Research Report 2004 Final Research Report Summary
  • [Journal Article] Muscularis inflammation and the loss of interstitial cells of Cajal in the endothelin ETB receptor null rat.2004

    • Author(s)
      Suzuki T
    • Journal Title

      Am J PhysiolGastrointestinal and Liver Physiology 287

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] New monoclonal antibody (AIC) identifies interstitial cells of Cajal in the musculature of the mouse gastrointestinal tract2004

    • Author(s)
      Torihashi S.
    • Journal Title

      Autonimic Neuroscience : Basic and Clinical 113

      Pages: 16-23

    • NAID

      120000974104

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Requirement of ryanodine receptors for pacemaker Ca^<2+> activity in ICC and HEK293 cells.2004

    • Author(s)
      Aoyama M
    • Journal Title

      J Cell Sci. 117

      Pages: 2813-2825

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Involvement of ryanodine receptors in pacemaker Cd^<2+> oscillation in murine gastric ICC.2004

    • Author(s)
      Liu HN
    • Journal Title

      Biochem Biophys Res Commun 328

      Pages: 640-646

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] 消化管のカハール介在細胞のペースメーカー機能2004

    • Author(s)
      鳥橋 茂子
    • Journal Title

      日本医事新報 4208

      Pages: 95-96

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Cell cluster標本を用いた腸管自動性メカニズムの研究2004

    • Author(s)
      中山 晋介
    • Journal Title

      日本薬理学雑誌 123

      Pages: 149-154

    • NAID

      10017019076

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Isolation and characterization of resident macrophages from the smooth muscle layers of murine small intestine.2004

    • Author(s)
      Ozaki H. et al.
    • Journal Title

      Neurogastroenterology and Motility 16

      Pages: 39-51

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] In vitro organogenesis of gut-like structures from mouse embryonic stem cells.2004

    • Author(s)
      Kuwahara M. et al.
    • Journal Title

      Neurogastroenterology and Motility 16 suppl

      Pages: 14-16

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Mnscularis inflammation and the loss of interstitial cells of Cajal in the endothelin ETB receptor null rat.2004

    • Author(s)
      Suzuki T. et al.
    • Journal Title

      Am J Physiol. 287

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] New monoclonal antibody(AIC) identifies interstitial cells of Cajal in the musculature of the mouse gastrointestinal tract2004

    • Author(s)
      Torihashi S. et al.
    • Journal Title

      Autonimic Neuroscience : Basic and Clinical 113

      Pages: 16-23

    • NAID

      120000974104

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Requirement of ryanodine receptors for pacemaker Ca2+ activity in ICC and HEK293 cells.2004

    • Author(s)
      Aoyama M. et al.
    • Journal Title

      J Cell Sci. 117

      Pages: 2813-2825

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Involvement of ryanodine receptors in pacemaker Ca^<2+> oscillation in murine gastric ICC.2004

    • Author(s)
      Liu HN.et al.
    • Journal Title

      Biochem Biophys Res Commun. 328

      Pages: 640-646

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Pacemaker functions of interstitial cells of Cajal.2004

    • Author(s)
      Torihashi S.
    • Journal Title

      Current report of the medicine 208

      Pages: 95-96

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Investigation of the spontaneous contraction mechanism using cell clusters2004

    • Author(s)
      Nakayama S.
    • Journal Title

      Journal of Pharmacological Sciences 123

      Pages: 149-154

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Roles of interstitial cells of Cajal as pacemakers and mediators of neurotransmission in the gastrointestinal muscle layer.2004

    • Author(s)
      Torihashi S.
    • Journal Title

      Japan Cancer Association(Japan Scientific Society Press, Tokyo Japan)

      Pages: 135-147

    • NAID

      10016246811

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Muscularis inflammation and the loss of interstitial cells of Cajal in the endothelin ETB receptor null rat.2004

    • Author(s)
      Suzuki T
    • Journal Title

      Am J Physiol Gastrointestinal and Liver Physiolog 287

    • Related Report
      2004 Annual Research Report
  • [Journal Article] New monoclonal antibody (AIC) identities interstitial cells of Cajal in the musculature of the mouse gastrointestinal tract.2004

    • Author(s)
      Torihashi S
    • Journal Title

      Autonimic Neuroscience : Basic and Clinical 113

      Pages: 16-23

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Requirement of ryanodine receptors for pacemaker Ca^<2+> activity in ICC and HEK293 cells.2004

    • Author(s)
      Aoyama M
    • Journal Title

      J Cell Sci 117

      Pages: 2813-2825

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Slow deactivation and U-shaped inactivation properties in cloned Cav1.2b channels in Chinese hamster ovary cells2003

    • Author(s)
      Aoyama M
    • Journal Title

      Biophys J. 84

      Pages: 709-724

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] 腸管平滑筋運動におけるカハールの介在細胞と壁内神経2003

    • Author(s)
      鳥橋 茂子
    • Journal Title

      臨床消火器化器内科 18

      Pages: 1371-1377

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Slow deactivation and U-shaped inactivation properties in cloned Cavl.2b channels in Chinese hamster ovary cells.2003

    • Author(s)
      Aoyama M. et al.
    • Journal Title

      Biophys J. 84

      Pages: 709-724

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Roles of interstitial cells of Cajal and enteric neurons for gastrointestinal motility.2003

    • Author(s)
      Torihashi S.
    • Journal Title

      Clinical gastroenterology 18

      Pages: 1371-1377

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Book] Methods in Molecular Biology Book Series. Nonhuman Embryonic Stem Cell Protocols.2005

    • Author(s)
      Torihashi S
    • Publisher
      The Humana Press Inc (in press)
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Book] Methods in Molecular Biology Book Series. Nonhuman Embryonic Stem Cell Protocols.2005

    • Author(s)
      Torihashi S, et al.
    • Publisher
      The Humana Press Inc(in press)
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Book] Roles of interstitial cells of Cajal as pacemakers and mediators of neurotransmission in the gastrointestinal muscle layer.2004

    • Author(s)
      Torihashi S.
    • Publisher
      Japan Cancer Association. Japan Scientific Society Press, Tokyo Japan
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Book] Monograph on Cancer Research No.53. Gastrointestinal Stromal Tumor (GIST) : from pathology to molecular target therapy.2004

    • Author(s)
      Torihashi S
    • Total Pages
      176
    • Publisher
      Japan Cancer Association. Japan Scientific Society Press. Tokyo Japan & KARGER Basel Switezerland.
    • Related Report
      2004 Annual Research Report
  • [Patent(Industrial Property Rights)] 消化管平滑筋のペースメーカー細胞に特異的なモノクローナル抗体2003

    • Inventor(s)
      鳥橋 茂子, 藤本 豊士
    • Industrial Property Rights Holder
      科学技術振興財団
    • Industrial Property Number
      2003-334959
    • Filing Date
      2003-09-26
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Publications] Ozaki H., Kawai T., Won K-J.et al.: "Isolation and characterization of resident macrophages from the smooth muscle layers of murine small intestine"Neurogastroeuterology and Motility. 16. 39-51 (2004)

    • Related Report
      2003 Annual Research Report
  • [Publications] Kuwahara M., Ogaeri T., Matsuura R., Kogo H., et al.: "In vitro organogenesis of gut-like structures from mouse embryonic stem cells"Neurogastroenterology and Motility. (in press). (2004)

    • Related Report
      2003 Annual Research Report

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Published: 2003-04-01   Modified: 2016-04-21  

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