• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Molecular Histo-cytochemical Analysis of Gene Expression Regulation by Artificial Control of Transcription Factors

Research Project

Project/Area Number 15590161
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field General anatomy (including Histology/Embryology)
Research InstitutionNagasaki University

Principal Investigator

IZUMI Shin-ichi  Nagasaki University, Graduate School of Biomedical Sciences, Instructor, 大学院・医歯薬学総合研究科, 助手 (40264246)

Co-Investigator(Kenkyū-buntansha) KOJI Takehiko  Nagasaki University, Graduate School of Biomedical Sciences, Professor, 大学院・医歯薬学総合研究科, 教授 (30170179)
TAKANO Kunio  Nagasaki University, Graduate School of Biomedical Sciences, Professor (from 2003 through 2004), 大学院・医歯薬学総合研究科, 教授 (80050029)
HISHIKAWA Yoshitaka  Nagasaki University, Graduate School of Biomedical Sciences, Associate Professor, 大学院・医歯薬学総合研究科, 助教授 (60304276)
Project Period (FY) 2003 – 2004
Project Status Completed (Fiscal Year 2004)
Budget Amount *help
¥3,500,000 (Direct Cost: ¥3,500,000)
Fiscal Year 2004: ¥1,400,000 (Direct Cost: ¥1,400,000)
Fiscal Year 2003: ¥2,100,000 (Direct Cost: ¥2,100,000)
KeywordsTranscription factor / Gene expression / Histochemistry / Estrogen / Prolactin / Pit-1 / DNA / Nucleus / Pit-1 / GH3 / ノックアウトマウス / ジエチルスチルベストロール
Research Abstract

1.Effects of exogenously administered estrogenic substances on prolactin(PRL) gene expression through the transcription factors, estrogen receptors(ERs) and Pit-1,were examined. PRL-and Pit-1-positive cells were increased, whereas FSH/LH-positive cells were decreased in the pituitaries of mice treated with diethylstilbestrol(DES). ER α was localized in the nuclei of PRL and FSH/LH cells hi the control mice, whereas hi DES-treated mice ER α -positive PRL cells were decreased and ER β-positive PRL cells were induced and then increased instead. In ER α-knockout mice ER β was localized in PRL cell nuclei and population of ER β, PRL or FSH/LH positive cells was constant. Treatment of wild-type mice with DES seemed to increase PRL gene transcription with an increase of both Pit-1 and ER β as well as a decrease of ER α hi the PRL cell nuclei. It suggests that quantity and quality of the transcription factors are regulated by DES.
2.We examined effects of artificial regulation on intranuclear distribution of the transcription factors in PRL producing GH3 culture cells. Pit-1 and ERs were localized to clarify whether PRL gene transcription was repressed when the double-stranded nucleotide sequences of PRL gene regulatory element (1P), which bind to Pit-1, were trans-inducted into the nuclei to block the 1P-binding sites. An autoclave retrieval was effective to enhance signals from the nuclear Pit-1 and ERs detected by Southwestern histochemistry and peroxidase-labeled antibody method, respectively, both at light- and electron microscopic levels. GH3 cells, in which Pit-1 and ER α were localized in the euchromatin region in the vicinity of the heterochromatin, possessed PRL. It appeared that Pit-1 and ER were distributed hi a definite nuclear region where the gene is expressed in GH3 cells. Optimal concentration and tune after the probe trans-induction were under examination.

Report

(3 results)
  • 2004 Annual Research Report   Final Research Report Summary
  • 2003 Annual Research Report
  • Research Products

    (33 results)

All 2005 2004 2003 Other

All Journal Article (21 results) Book (6 results) Publications (6 results)

  • [Journal Article] Intense immuno-labeling of tryptophan 2,3-dioxygenase in the rat hepatocyte cytosol after treatment of cortisone and/or tryptophan2005

    • Author(s)
      Izumi S, Watanebe K
    • Journal Title

      Journal of Electron Microscopy Technology for Medicine and Biology 18(2)

      Pages: 69-75

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Realization of sites of specific molecules in the cell organelles : electron microscopic techniques for histochemical methods2005

    • Author(s)
      Izumi S
    • Journal Title

      Journal of Electron Microscopy Technology for Medicine and Biology 19(1)

      Pages: 107-109

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Realization of sites of specific molecules in the cell organelles : electron microscopic techniques for histo chemical methods2005

    • Author(s)
      Izumi S
    • Journal Title

      Journal of Electron Microscopy Technology for Medicine and Biology 19(1)

      Pages: 107-109

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Southwestern histochemistry : a method for localization of transcription factors.2005

    • Author(s)
      Izumi S, et al.
    • Journal Title

      Molecular morphology in human tissues : techniques and applications(CRC Press, Washington, D.C.)

      Pages: 133-146

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Southwestern histochemistry : a method for localization of transcription factors2005

    • Author(s)
      Izumi S, et al.(分担執筆)
    • Journal Title

      In : Molecular morphology in human tissues : techniques and applications, CRC Press, Washington D.C.

      Pages: 133-146

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Immunohistochemical localization of the ACTH (MC-2) receptor in the rat placenta and adrenal gland2004

    • Author(s)
      Izumi S, et al.
    • Journal Title

      Archives of Histology and Cytology 67(5)

      Pages: 443-453

    • NAID

      10014483253

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Immunohistochemical identification of HSP47 and type I collagen-expressing cells in Mongolian gerbil salivary glands : correlation with age-related fibrotic changes2004

    • Author(s)
      Tomisaki H, et al.
    • Journal Title

      Oral Medicine and Pathology 9(4)

      Pages: 141-148

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] In situ hybridization of prolactin mRNA in rat pituitary sections : backscattered electron mode analysis using light microscopic specimen on glass slides2004

    • Author(s)
      Izumi S, et al.
    • Journal Title

      Advancement of Life Science(Amvo Publishing Company, Taipei)

      Pages: 153-158

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Molecular histochemistry of rRNAs by nonradioactive in situ hybridization at light- and electron-microscopic levels.2004

    • Author(s)
      Koji T, et al.
    • Journal Title

      Advancement of Life Science(Amvo Publishing Company, Taipei)

      Pages: 141-146

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Southwestern histochemical analysis of estrogen responsive element binding protein in paraffin-embedded sections of mouse ovary.2004

    • Author(s)
      Hishikawa Y, et al.
    • Journal Title

      Advancement of Life Science(Amvo Publishing Company, Taipei)

      Pages: 147-152

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] In situ hybridization of prolactin mRNA in rat pituitary sections : backscattered electron mode analysis using light microscopic specimen on glass slides2004

    • Author(s)
      Izumi S, et al.
    • Journal Title

      In : Advancement of Life Science Amvo Publishing Company, Taipei

      Pages: 153-158

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Southwestern histochemical-analysis of estrogen responsive element binding protein in paraffin-embedded sections of mouse ovey2004

    • Author(s)
      Hishikawa Y et al.
    • Journal Title

      In : Advancement of life Science Amvo Publishing Company, Taipei

      Pages: 147-152

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Molecular histochemistry of rRNAs by nonradioactive in situ hybridization at light and electron-microscopic levels2004

    • Author(s)
      Koji T, et al.
    • Journal Title

      In : Advancement of Life Science Amvo Publishing Company, Taipei

      Pages: 141-146

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Southwestern histochemical distribution of transcription factors, Pit-1, PREB and estrogen receptors, reflection of prolactin gene expression in anterior pituitary cells2004

    • Author(s)
      Izumi S, et al.
    • Journal Title

      Anatomical Science International 79

      Pages: 423-423

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Southwestern histochemical localization of tesnscription factors, Pit-1 and PREB, in the anterior pituitarycell nuclei2004

    • Author(s)
      Izumi S, et al.
    • Journal Title

      Proceedings of the 8th Asia-Pacific Conference on Electron Microscopy

      Pages: 1332-1333

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Differential analysis of active and inactive genes in human neutrophils by chromosomal in situ hybridization2003

    • Author(s)
      Izumi S, et al.
    • Journal Title

      Acta Histochemica et Cytochemica 36(4)

      Pages: 325-330

    • NAID

      110003144286

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Molecular histochemical analysis of estrogen receptor a and β expressions in the mouse ovary : in situ hybridization and Southwestern histochemistry2003

    • Author(s)
      Hishikawa Y, et al.
    • Journal Title

      Medical Electron Microscopy 36

      Pages: 67-73

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] 核内RNAの電顕in situ hybridizationによる検出技法2003

    • Author(s)
      末松貴史, 和泉伸一, 他
    • Journal Title

      医学生物学電子顕微鏡技術学会誌 17(1)

      Pages: 2-5

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Molecular histochemical analysis of estrogen receptor α and β expressions in the mouse ovary : in situ hybridization and Southwestern histochemistry2003

    • Author(s)
      Hishikawa Y, et al.
    • Journal Title

      Medical Electron Microscopy 36

      Pages: 67-73

    • NAID

      10011996272

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Electron microscopic in situ hybridization of Nuclear RNAs2003

    • Author(s)
      Suematsu T, Izumi S, et al.
    • Journal Title

      Journal of Electron Microscopy Technology for Medicine and Biology 17(1)

      Pages: 2-5

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] In situ hybridization for beginners.2003

    • Author(s)
      Izumi S, Koji T
    • Journal Title

      Histocytochemistry 2004(Gakusaikikaku, Tokyo)

      Pages: 133-146

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Book] Southwestern histochemistry : a method for localization of transcription factors In : Molecular morphology in human tissues : techniques and applications2005

    • Author(s)
      Izumi S, et al.
    • Publisher
      CRC Press, Washington, D.C
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Book] In situ hybridization of prolactin mRNA in rat pituitary sections : backscattered electron mode analysis using light microscopic specimen on glass slides. In : Advancement of Life Science2004

    • Author(s)
      Izumi S, et al.
    • Publisher
      Amvo Publishing Company, Taipei
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Book] Molecular histochemistry of rRNAs by nonradioactive in situ hybridization at light- and electron-microscopic levels. In : Advancement of Life Science2004

    • Author(s)
      Koji T, et al.
    • Publisher
      Amvo Publishing Company, Taipei
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Book] Southwestern histochemical analysis of estrogen responsive element binding protein in paraffin-embedded sections of mouse ovary, In : Advancement of Life2004

    • Author(s)
      Hishikawa Y, et al.
    • Publisher
      Amvo Publishing Company, Taipei
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Book] 初心者のためのin situ hybridization法、「組織細胞化学2004」(日本組織細胞化学学会編)2004

    • Author(s)
      和泉伸一 他(分担執筆)
    • Publisher
      学際企画、東京
    • Related Report
      2004 Annual Research Report
  • [Book] 初心者のためのin situ hybridization法、「組織細胞化学2004」2003

    • Author(s)
      和泉伸一, 小路武彦
    • Publisher
      学際企画、東京
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Publications] Shin-ichi Izumi, et al.: "Differential analysis of active and inactive genes in human neutrophils by chromosomal in situ hybridization"Acta Histochemica et Cytochemica. 36(4). 325-330 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Yoshitaka Hishikawa, et al.: "Molecular histochemical analysis of estrogen receptor α and β expressions in the mouse ovary : in situ hybridization and Southwestern histochemistry"Med Electron Microsc. 36. 67-73 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] 末松貴史 他: "核内RNAの電顕in situ hybridizationによる検出技法"医学生物学電子顕微鏡技術学会誌. 17(1). 2-5 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Shin-ichi Izumi, et al.: "Simulataneous localization of estrogen receptor and Pit-1 in the nucleus of prolactin cell"Acta Anatomica Nipponica. 78(Supl). 350 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] 宿輪恵子 他: "ジエチルスチルベストロールのマウス下垂体ホルモン発現への影響とエストロゲン受容体の発現動態"解剖学雑誌. 78(抄録号). 294 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] 和泉伸一 他: "電顕in situハイブリダイゼーションのための固定液と包埋剤"電子顕微鏡. 38(Supl). 93 (2003)

    • Related Report
      2003 Annual Research Report

URL: 

Published: 2003-04-01   Modified: 2016-04-21  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi