| Project/Area Number |
15590164
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
General anatomy (including Histology/Embryology)
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| Research Institution | Kagoshima University |
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Principal Investigator |
TSUYAMA Shinichiro Kagoshima University, Graduate School of Medical and Dental Sciences, Associate Professor, 大学院・医歯学総合研究科, 助教授 (30041346)
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| Co-Investigator(Kenkyū-buntansha) |
SIMIZU Takao Kagoshima University, Graduate School of Medical and Dental Sciences, Assistant Professor, 大学院・医歯学総合研究科, 講師 (10041336)
NITTA Tetsuya Kagoshima University, Graduate School of Medical and Dental Sciences, Research Associate, 大学院・医歯学総合研究科, 助手 (10325813)
MAEZONO Rie Kagoshima University, Graduate School of Medical and Dental Sciences, Research Associate, 大学院・医歯学総合研究科, 助手 (90381178)
村田 長芳 鹿児島大学, 大学院・医歯学総合研究科, 教授 (60020765)
松下 幸恵 鹿児島大学, 大学院・医歯学総合研究科, 助手 (80325787)
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| Project Period (FY) |
2003 – 2005
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| Project Status |
Completed (Fiscal Year 2005)
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| Budget Amount *help |
¥3,600,000 (Direct Cost: ¥3,600,000)
Fiscal Year 2005: ¥1,300,000 (Direct Cost: ¥1,300,000)
Fiscal Year 2004: ¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 2003: ¥1,300,000 (Direct Cost: ¥1,300,000)
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| Keywords | Stomach / Parietal cell / Proton pump / Distribution in gland / Ezrin / Chloride channel / High pressure freezing / Freeze substitution / プトトンポンプ / 胃底腺 / 胃塩酸分泌 / 休止 / H^+K^+-ATPase / ガストリン / オメプラゾール / 急速凍結 |
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| Research Abstract |
The main purpose of this study was to ascertain whether or not the cells are functionally similar throughout the gland, and how transformation mechanism with cytoskeletal components is related to the distribution of ezrin molecules and chloride channel. In this study, the specimens were processed with cryofixation method showed good morphological preservation and retention of soluble components at the light and electron microscopic level. To suppress severe ice-crystal damage or loss of their immunocytochemical properties, stomach tissues from ad libitum-fed or starved rats were prepared with rapid freezing followed by freeze-substitution for light microscopy, and with high pressure freezing for electron microscopy. The transformable structure of parietal cell was examined between the active and resting phases of acid secretion. These structures were the dilated intracellular canaliculi, including numerous microvilli in the secreting phase, and the developed tubulovesicles in apical cy
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toplasm at resting phase. The immunohistochemical staining with anti-alpha- or beta-subunits of H^+・K^+-ATPase antibodies was localized on the cells from both phases. Labeling was more intense and clear on the membranes of intracellular canaliculi, microvilli and tubulovesicles than that with chemical fixation. The positively stained parietal cells were scattered widely throughout the gland. The cells in the secreting phase located in the upper one third of the gland were densely stained with an anti-phosphorylated ezrin antibody. In the resting phase, the antibody showed a weak reaction. However, the anti-nonphosphorylated ezrin antibody hardly labeled those cells. Anti-chloride channel antibody immunostaining showed a narrow zone of positive reaction around the neck region in the secreting phase. These labelings were observed on the membrane of intracellular canaliculi, microvilli and tubulovesicles. It was concluded that the cells in around the upper one third are more active cells for functional secretion than those of other segments, while parietal cells were distributed throughout the gastric gland. Less
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