| Project/Area Number |
15590177
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (C)
|
| Allocation Type | Single-year Grants |
|---|
| Section | 一般 |
|---|
| Research Field |
General anatomy (including Histology/Embryology)
|
|---|
| Research Institution | Fujita Health University |
|---|
Principal Investigator |
NOMURA Ryuji Fujita Health University, School of Medicine, Assistant Professor, 医学部, 講師 (20325161)
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
SENDA Takao Fujita Health University, School of Medicine, Professor, 医学部, 教授 (10187875)
|
|---|
| Project Period (FY) |
2003 – 2005
|
| Project Status |
Completed (Fiscal Year 2005)
|
| Budget Amount *help |
¥3,600,000 (Direct Cost: ¥3,600,000)
Fiscal Year 2005: ¥1,200,000 (Direct Cost: ¥1,200,000)
Fiscal Year 2004: ¥1,200,000 (Direct Cost: ¥1,200,000)
Fiscal Year 2003: ¥1,200,000 (Direct Cost: ¥1,200,000)
|
|---|
| Keywords | coronavirus / raft / caveola / cholesterol / caveolin / fibroblast |
|---|
| Research Abstract |
CD13, a receptor for human coronavirus (HCoV)-229E, was identified as a major component of the Triton X-100-resistant membrane microdomain in human fibroblasts. Incubation of living fibroblasts with an anti-CD13 antibody on ice gave punctate labeling that was evenly distributed on the cell surface, but raising the temperature to 37℃ before fixation caused aggregation of the labeling. The aggregated labeling of CD13 colocalized with caveolin-1 in most cells. The HCoV-229E virus particle showed a binding and redistribution pattern similar to the anti-CD13 antibody : the virus bound to the cell evenly when incubated on ice, but became colocalized with caveolin-1 at 37℃ ; importantly, the virus also caused sequestration of CD13 to the caveolin-1-positive area. Electron microscopy confirmed that HCoV-229E was localized near or at the orifice of caveolae after the incubation at 37℃. Depletion of plasmalemmal cholesterol with methyl □-cyclodextrin significantly reduced the HCoV-229E redistribution and the subsequent infection. Caveolin-1 knockdown by RNA interference also reduced the HCoV-229E infection considerably. The result indicates that HCoV-229E first binds to CD13 in the Triton X-100-resistant microdomain, clusters CD13 by cross-linking, and thereby reaches the caveolar region before entering cells.
|
