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Studies on physiological significance of hyperpolarization-activated cation channels

Research Project

Project/Area Number 15590185
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field General physiology
Research InstitutionGraduate School of Medicine, Kyoto University

Principal Investigator

ISHII Takahiro  Kyoto University, Graduate School of Medicine, Associate Professor, 医学研究科, 助教授 (40303812)

Co-Investigator(Kenkyū-buntansha) 古谷野 好  京都大学, 医学研究科, 助教授 (50183041)
鷹野 誠  自治医科大学, 医学研究科, 教授 (30236252)
Project Period (FY) 2003 – 2005
Project Status Completed (Fiscal Year 2005)
Budget Amount *help
¥3,600,000 (Direct Cost: ¥3,600,000)
Fiscal Year 2005: ¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 2004: ¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 2003: ¥2,200,000 (Direct Cost: ¥2,200,000)
Keywordshyperpolarization / channel / Ih / HCN / homologous recombination / tetracycline / 相同組替 / 相同組換え
Research Abstract

Hyperpolarization-activated cyclic nucleotide-gated (HCN) channels contribute to physiological functions such as regulating cell excitability, eliciting rhythmic activities and so on. Four subtypes (HCN1-4) have ever been identified. We previously cloned HCN4, which shows the slowest activation kinetics and the profound modulation by cAMP. To investigate the physiological roles of HCN4, we have generated a transgenic (Tg) mouse, in which tetracycline repressor protein (TetR) is expressed under the control of HCN4 promoter. This mouse was crossbred with another Tg mouse, in which GFP expression is regulated by TetR. Thus, generated Tg mouse expressed GFP in HCN4-expressing cells. We observed on this Tg mouse revealed that GFP fluorescence and HCN4 immunoreactivity (HCN4-IR) were colocalized in the cerebrum, the sino-atrial node, the taste buds and the retina, all of which were previously confirmed to express HCN4. The olfactory receptor neurons (ORN) expressed GFP, where only HCN current (Ih) had been formerly reported, however, its physiological roles remain unknown. HCN4-IR was detected in the olfactory knobs, the soma and the axon bundles of the ORN. We also found that HCN2 was expressed in the axon bundles of the ORN. Electrophysiological studies are in progress to analyze the physiological properties of HCN channels in the ORN.

Report

(4 results)
  • 2005 Annual Research Report   Final Research Report Summary
  • 2004 Annual Research Report
  • 2003 Annual Research Report
  • Research Products

    (11 results)

All 2005 2004 2003 Other

All Journal Article (10 results) Publications (1 results)

  • [Journal Article] Hyperpolarization-Activated Cyclic Nucleotide-Gated Cation Channels Regulate Auditory Coincidence Detection in Nucleus Laminaris of the Chick2005

    • Author(s)
      Rei Yamada, Hiroshi Kuba, Takahiro M.Ishii, Harunori Ohmori
    • Journal Title

      J. Neuroscience 25

      Pages: 8867-8877

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] A novel Ca^<2+>indicator protein using FRET and calpain-sensitive linker2005

    • Author(s)
      Kenji Takatsuka, Takahiro M.Ishii, Harunori Ohmori
    • Journal Title

      Biochem. Biophys. Res. Comm. 336

      Pages: 316-323

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Hyperpolarization-Activated Cyclic Nucleotide-Gated Cation Channels Regulate Auditory Coincidence Detection in Nucleus Laminaris of the Chick2005

    • Author(s)
      Rei Yamada, Hiroshi Kuba, Takahiro M.Ishii, Harunori Ohmori
    • Journal Title

      J.Neuroscience 25

      Pages: 8867-8877

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Annual Research Report 2005 Final Research Report Summary
  • [Journal Article] A novel Ca2+ indicator protein using FRET and calpain-sensitive linker2005

    • Author(s)
      Kenji Takatsuka, Takahiro M. Ishii, Harunori Ohmori
    • Journal Title

      Biochem.Biophys.Res.Comm. 336

      Pages: 316-323

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] A novel Ca2+ indicator protein using FRET and calpain-sensitive linker2005

    • Author(s)
      Kenji Takatsuka, Takahiro M.Ishii, Harunori Ohmori
    • Journal Title

      Biochem.Biophys.Res.Comm. 336

      Pages: 316-323

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Helical Structure of Si in the Hyperpolarization-Activated Cation Channel2004

    • Author(s)
      Takahiro Ishii, Harunori Ohmori
    • Journal Title

      Neuroscience Research 50 Suppl.1

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Molecular Determinants of Deactivation Kinetics in Hyperpolarization-Activated Cation Channels2004

    • Author(s)
      Ishii, Takahiro, Takano, Makoto, Ohmori, Harunori
    • Journal Title

      The Japanese Journal of Physiology 54(Suppl.)

    • NAID

      130005446947

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Roles of hyperpolarization-activated cation channels as a regulator of the EPSP time course in nucleus laminaris of the chick2004

    • Author(s)
      Yamada, Rei, Kuba, Hiroshi, Ishii, Takahiro, Ohmori, Harunori
    • Journal Title

      The Japanese Journal of Physiology 54(Suppl.)

    • NAID

      130005447034

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Halothane Inhibits an Intermediate Conductance Ca^<2+>-activated K+ Channel by Acting at the Extracellular Side of Ionic Pore2003

    • Author(s)
      Mitsuko Hashiguchi-Ikeda, Tsunehisa Namba, Takahiro M.Ishii, Taizo Hisano, Kazuhirko Fukuda
    • Journal Title

      Anesthesiology 99

      Pages: 1340-5

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] 「研究成果報告書概要(欧文)」より2003

    • Author(s)
      Mitsuko Hashiguchi-Ikeda, Tsunehisa Namba, Takahiro M.Ishii, Taizo Hisano, Kazuhiko Fukuda
    • Journal Title

      Anesthesiology 99

      Pages: 1340-1345

    • Related Report
      2005 Final Research Report Summary
  • [Publications] Mitsuko Hashiguchi-Ikeda, Tsunehisa Namba, Takahiro M. Ishii, Taizo Hisano, Kazuhiko Fukuda: "Halothane Inhibits an Intermediate Conductance Ca^<2+>-activated K^+ Channel by Acting at the Extracellular Side of the Ionic Pore"Anesthesiology. 99・6. 1340-1345 (2003)

    • Related Report
      2003 Annual Research Report

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Published: 2003-04-01   Modified: 2016-04-21  

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