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Regulatory mechanism of Na+ reabsorption by hypotonicity through regulatory volume decrease-dependent dephosphorylation of FAK.

Research Project

Project/Area Number 15590189
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field General physiology
Research InstitutionKyoto Prefectural University of Medicine

Principal Investigator

NIISATO Naomi  Kyoto Prefectural University of Medicine, Physiology, Assistant Professor, 医学研究科, 講師 (00237645)

Co-Investigator(Kenkyū-buntansha) MARUNAKA Yoshinori  Kyoto Prefectural University of Medicine, Physiology, Professor, 医学研究科, 教授 (00127036)
MIYAZAKI Hiroaki  Kyoto Prefectural University of Medicine, Physiology, Research Associate, 医学研究科, 助手 (30360027)
Project Period (FY) 2003 – 2004
Project Status Completed (Fiscal Year 2004)
Budget Amount *help
¥3,200,000 (Direct Cost: ¥3,200,000)
Fiscal Year 2004: ¥900,000 (Direct Cost: ¥900,000)
Fiscal Year 2003: ¥2,300,000 (Direct Cost: ¥2,300,000)
KeywordsTyrosine dephophorylation / [Cl^-]_c / NPPB / Regulatory volume decrease / Foacal adhesion kinase / Hypotonic shock / forcal adhesion kinase / チロシン脱リン酸化酵素 / イオン輸送
Research Abstract

Epithelial Na^+ transport in the kidney is important for control of blood pressure and extracellular fluid volume and is regulated by plasma osmolality and hormones. We have already indicated that Na^+ reabsorption is stimulated by extracellular hypotonicity in renal epithelial A6 cell that is a model cell line to study regulatory mechanism of Na^+ reabsorpption. However, the regulatory mechanism behind this is not well understood. Hypotonic shock causes the initial cell swelling followed by regulatory volume decrease(RVD) which is involved in the decrease in cytosolic Cl^- concentration ([Cl^-]_c). Our aim in this study is to clarify the hypothesis that RVD has a crucial role in hyposmotic regulation of Na^+ reabsorption in renal epithelium. We found that 1)hypotonic shock reduced [Cl^-]_c through RVD process by measuring [Cl^-]_c with Cl^- fluorescence dye (N-(6-methoyquinolyl) acetoxy-acetyl-ester, MQAE), 2)hypotonic shock caused dephosphoryaltion of foacal adhesion kinase(FAK) which was abolished by blockade of RVD by NPPB (a Cl^- channel blocker), 3)blockade of RVD caused the sustained tyrosine phosphorylation of FAK, 4)hypotonicity-induced Na^+ reabsorption and epithelial Na^+ channel gene expression were abolished by blocking RVD by NPPB. Taken together these results, it is suggested that hypotonic shock stimulated Na^+ reabsorption RVD-dependent FAK tyrosine dephosphorylation in renal epithelial A6 cells.

Report

(3 results)
  • 2004 Annual Research Report   Final Research Report Summary
  • 2003 Annual Research Report
  • Research Products

    (14 results)

All 2005 2004 2003 Other

All Journal Article (12 results) Publications (2 results)

  • [Journal Article] Benzamil, a blocker of epithelial Na+ channel-induced upregulation of artery oxygen pressure level in acute lung injury rabbit ventilated with high frequency oscillation.2005

    • Author(s)
      N Taguchi, N Niisato, Y Sawabe, H Miyazaki, Y Hirai, Y Marunaka
    • Journal Title

      Biochem Biophys Res Commun 327

      Pages: 915-919

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Annual Research Report 2004 Final Research Report Summary
  • [Journal Article] New concept of spare receptors and effectors2005

    • Author(s)
      Marunaka Y, Niisato N, Miyazaki H
    • Journal Title

      J Membrane Biol (in press)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Annual Research Report 2004 Final Research Report Summary
  • [Journal Article] Benzamil, a blocker of epithelial Na(^+) channel-induced upregulation of artery oxygen pressure level in acute lung injury rabbit ventilated with high frequency oscillation.2005

    • Author(s)
      Taguchi N, NIISATO N, Sawabe Y, Miyazaki H, Hirai Y, Marunaka Y.
    • Journal Title

      Biochem Biophys Res Commun. 327

      Pages: 915-919

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Cross talk of cAMP and flavone in regulation of cystic fibrosis transmembrane conductance regulator (CFTR) Cl- channel and Na+/K+/2Cl- cotransporter in renal epithelial A6 cells2004

    • Author(s)
      Naomi Niisato, Hoyoku Nishino, Kyosuke Nishio, Yoshinori Marunaka
    • Journal Title

      Biochemical Pharmacology 67

      Pages: 795-801

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Flavonoid-induced ENaC expression in the kidney of Dahl salt-sensitive rat.2004

    • Author(s)
      Wataru Aoi, Naomi Niisato, Hiroaki Miyazaki, Yoshinori Marunaka
    • Journal Title

      Biochem Biophys Res Commun 315

      Pages: 892-896

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Annual Research Report 2004 Final Research Report Summary
  • [Journal Article] Involvement of cytosolic Cl- in osmoregulation of alpha-ENaC gene expression.2004

    • Author(s)
      Niisato N, Eaton DC, Marunaka Y
    • Journal Title

      Am J Physiol Renal Physiol. 287

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Annual Research Report 2004 Final Research Report Summary
  • [Journal Article] Cross talk of cAMP and flavone in regulation of cystic fibrosis transmembrane conductance regulator (CFTR) Cl^- channel and Na^+/K^+/2Cl^- cotransporter in renal epithelial A6 cells.2004

    • Author(s)
      NIISATO N, Nishino H, Nishio K, Marunaka Y.
    • Journal Title

      Biochem Pharmacol. 67

      Pages: 795-801

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Flavonoid-induced reduction of ENaC expression in the kidney of Dahl salt-sensitive hypertensive rat.2004

    • Author(s)
      Aoi W, NIISATO N, Miyazaki H, Marunaka Y.
    • Journal Title

      Biochem Biophys Res Commun 315

      Pages: 892-896

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Involvement of cytosolic Cl^- in osmoregulation of alpha-ENaC gene expression.2004

    • Author(s)
      NIISATO N, Eaton DC, Marunaka Y.
    • Journal Title

      Am J Physiol Renal Physiol. 287

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Cross talk of cAMP and flavone in regulation of cyctic fibrosis transmembrane conductance regulator (CFTR) Cl- channel and Na+/K+/2Cl- cotransporter in renal epithelial A6 cells2004

    • Author(s)
      Naomi Niisato, Hoyoku Nishino, Kyosuke Nishio, Yoshinori Marunaka
    • Journal Title

      Biochemical Pharmacology 67

      Pages: 795-801

    • Related Report
      2004 Annual Research Report
  • [Journal Article] H89, an inhibitor of protein kinase A(PKA), stimulates Na^+ transport by translocating an epithelial Na^+ channel(ENaC) in fetal rat alveolar type II epithelium.2003

    • Author(s)
      Marunaka Y, NIISATO N.
    • Journal Title

      Biochem Pharmacol. 66

      Pages: 1083-1089

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] New concenpt of spare receptors and effectors.

    • Author(s)
      Marunaka Y, NIISATO N, Miyazaki H.
    • Journal Title

      J Membrane Biol. (In press)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Publications] Naomi Niisato, Hoyoku Nishino, Kyosuke Nishio, Yoshinori Marunaka: "Cross talk of cAMP and flavone in regulation of cyctic fibrosis transmembrane conductance regulator (CFTR) Cl- channel and Na+/K+/2Cl- cotransporter in renal epithelial A6 cells"Biochemical Pharmacology. 67. 795-801 (2004)

    • Related Report
      2003 Annual Research Report
  • [Publications] Wataru Aoi, Naomi Niisato, Hiroaki Miyazaki, Yoshinori Marunaka: "Flavonoid-induced ENaC expression in the kidney of Dahl salt-sensitive rat."Biochem Biophys Res Commun. 315. 892-896 (2004)

    • Related Report
      2003 Annual Research Report

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Published: 2003-04-01   Modified: 2016-04-21  

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