• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Expression of t-PA receptor on the endothelial cell and analysis of its physiological function.

Research Project

Project/Area Number 15590198
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field General physiology
Research InstitutionKinki University

Principal Investigator

MATSUO Osamu  Kinki University, School of Medicine Department of Physiology, Professor, 医学部, 教授 (40030879)

Co-Investigator(Kenkyū-buntansha) UESHIMA Shigeru  Kinki University, Department of Agriculture, Professor, 農学部, 教授 (30193791)
OKADA Kiyotaka  Kinki University, School of Medicine Department of Physiology, Lecturer, 医学部, 講師 (20185432)
KAWAO Naoyuki  Kinki University, School of Medicine Department of Physiology, Assistant, 医学部, 助手 (70388510)
OKAMOTO Chikako  Kinki University, School of Medicine Department of Physiology, Assistant, 医学部, 助手 (90368291)
Project Period (FY) 2003 – 2004
Project Status Completed (Fiscal Year 2004)
Budget Amount *help
¥3,600,000 (Direct Cost: ¥3,600,000)
Fiscal Year 2004: ¥1,700,000 (Direct Cost: ¥1,700,000)
Fiscal Year 2003: ¥1,900,000 (Direct Cost: ¥1,900,000)
Keywordsfibrinolytic system / vascular endothelial cell / tissue type plasminogen activator (t-PA) / t-PA receptor / 遺伝子組換え
Research Abstract

We demonstrated previously that tissue-type plasminogen activator (t-PA) bound to its specific receptor (t-PAR) on human umbilical vein endothlial cells (HUVEC) and that t-PAR interacted only with t-PA to form t-PA/ t-PAR complex. To investigate the function of t-PAR, we synthesized recombinant t-PAR (rt-PAR). Furthermore, the binding ability of t-PA to t-PAR on vascular endothelial cells was studied by using IAsys.
The cDNA coding region for t-PAR was cloned into glutathione S-transferase (GST) expression vector. The target protein was expressed as the fusion protein with GST. The mature rt-PAR was obtained from cleaving fusion protein by protease. The ligand blot analysis revealed that t-PA specifically bound to rt-PAR.
On the interaction analysis using IAsys, the binding signal of HUVEC to t-PA elevated in the cell concentration manner. It is considered that the t-PAR may concentrate t-PA on the surface of HUVEC and enhance the fibrinolytic properties around them.
When HUVEC were treated with EACA, the binding signal of HUVEC to t-PA decreased in an EACA dependent manner. It was suggested that the binding of t-PA to endothelial cell was mediated via lysine binding site in t-PA molecule. On the other hand, when the endothelial cell was treated with receptor associated protein, the binding parameter did not change. This result indicated that low density lipoprotein receptor related protein was not involved in the binding of t-PA to HUVEC. Furthermore, polyclonal antibody to annexin II and that to α ^-enolase did not interfere the interaction between t-PA and HUVEC. It concluded that t-PAR is the novel protein which is different from annexin II and α ^-enolase.

Report

(3 results)
  • 2004 Annual Research Report   Final Research Report Summary
  • 2003 Annual Research Report
  • Research Products

    (11 results)

All 2004 2003 Other

All Journal Article (6 results) Publications (5 results)

  • [Journal Article] The regulation of liver regeneration by the plasmin/α_2-antiplasmin system2004

    • Author(s)
      Kiyotaka Okada
    • Journal Title

      Journal of Hepatology 40

      Pages: 110-116

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Growth inhibition of vascular smooth muscle cells derived from urokinase receptor (u-PAR)-deficient mice in the presence of carcinoma cells2004

    • Author(s)
      Shigeru Ueshima
    • Journal Title

      Thromb Res 113

      Pages: 41-49

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Growth inhibition of vascular smooth muscle cells derived from urokinase receptor (u-PAR)- deficient mice in the presence of carcinoma cells2004

    • Author(s)
      Shigeru Ueshima
    • Journal Title

      Thromb Res 113

      Pages: 41-49

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Annual Research Report 2004 Final Research Report Summary
  • [Journal Article] The regulation of liver regeneration by the plasmin/α_2-antiplasmin system2004

    • Author(s)
      Kiyotaka Okada
    • Journal Title

      Jourral of Hepatology 40

      Pages: 110-116

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Lack of α_2-antiplasmin promotes re-endothelialization via over-release of VEGF after vascular injury in mice2003

    • Author(s)
      Hiroyuki Matsuno
    • Journal Title

      Blood 102

      Pages: 3621-3628

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Annual Research Report 2004 Final Research Report Summary
  • [Journal Article] α_2-antiplasmin a significant role in acute pulmonary embolism2003

    • Author(s)
      Hiroyuki Matsuno
    • Journal Title

      J Thromb Haemost 1

      Pages: 1734-1739

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Annual Research Report 2004 Final Research Report Summary
  • [Publications] Hiroyuki Matsuno: "Lack of α_2-antiplasmin promotes re-endothelialization via over-release of VEGF after vascular injury in mice"Blood. 102・10. 3621-3628 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Makoto Akao: "Cellular density regulation of plasminogen gene expression in mouse hepatocytes"Life Sciences. 72・15. 1695-1704 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Hiroyuki Matsuno: "α_2-antiplasmin plays a significant role in acute pulmonary"J Thromb Haemost. 1・8. 1734-1739 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Kiyotaka Okada: "The regulation of liver regeneration by the plasmin/α_2-antiplasmin system"J Hepatology. 40・1. 110-116 (2004)

    • Related Report
      2003 Annual Research Report
  • [Publications] Shigeru Ueshima: "Growth inhibition of vascular smooth muscle cells derived from urokinase receptor (u-PAR)-deficient mice in the presence of carcinoma cells"Thromb Res. (in press). (2004)

    • Related Report
      2003 Annual Research Report

URL: 

Published: 2003-04-01   Modified: 2016-04-21  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi