• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Analysis of the neuroendocrine system in PAC1 knock out mice

Research Project

Project/Area Number 15590216
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Environmental physiology (including Physical medicine and Nutritional physiology)
Research InstitutionUniversity of Occupational and Environmental Health

Principal Investigator

SHIBUYA Izumi  University of Occupational and Environmental Health, School of Medicine, Associate Professor, 医学部, 助教授 (50162649)

Co-Investigator(Kenkyū-buntansha) UEZONO Yasuhito  Nagasaki University, School of Medicine, Assotiate Professor, 大学院・医歯薬学総合研究科, 助教授 (20213340)
Project Period (FY) 2003 – 2004
Project Status Completed (Fiscal Year 2004)
Budget Amount *help
¥3,500,000 (Direct Cost: ¥3,500,000)
Fiscal Year 2004: ¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 2003: ¥2,500,000 (Direct Cost: ¥2,500,000)
KeywordsPACAP / PACAP typeI receptor / receptor knock out mouse / supraoptic nucleus / Ca^<2+> imaging / somatodendritic release / vasopressin / oxytocin / 下垂体後葉 / 細胞内Ca^<2+> / Ca^<2+> clearance / Somatodendritic release / Signal transduction / PACAP typeI receptor / receptor knock out mouse / supraoptic nucleus / Ca^<2+> imaging / vasopressin / oxytocin
Research Abstract

The role of pituitary adenylate cyclase-activating polypeptide (PACAP) type I receptor (PAC1 receptor) in regulating hypothalamic supraoptic neurones was investigated using PAC1 receptor-deficient male mice (PAC1-/-). The effects of PACAP on [Ca2+]i were investigated in freshly dissociated supraoptic neurones and on the somatodendritic release of vasopressin and oxytocin, examined on intact supraoptic nuclei. In supraoptic neurones from wild-type mice (PAC1+/+), 100 nm PACAP induced an increase in [Ca2+]i and release of vasopressin and oxytocin, whereas in heterozygous (PAC1+/-) and null-mutant mice (PAC1-/-), PACAP was much less effective. PACAP had no effect on these two parameters when applied to isolated neurohypophysial nerve terminals of PAC l+/+ and PAC1-/- mice, and rats. In conclusion, the PAC1 receptor is solely responsible for the PACAP-induced [Ca2+]i signalling and secretion of vasopressin and oxytocin in the somatodendritic region of supraoptic neurones.

Report

(3 results)
  • 2004 Annual Research Report   Final Research Report Summary
  • 2003 Annual Research Report
  • Research Products

    (23 results)

All 2005 2004 2003 Other

All Journal Article (17 results) Book (1 results) Publications (5 results)

  • [Journal Article] DNA topoisomerase II poison TAS-103 transactivates GC-box-dependent transcription via acetylation of Sp1.2005

    • Author(s)
      Torigoe T., et al.
    • Journal Title

      The Journal of Biological Chemistry 280(2)

      Pages: 1179-1185

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Ca(2+) clearance mechanisms in neurohypophysial terminals of the rat.2005

    • Author(s)
      Sasaki N., et al.
    • Journal Title

      Cell Calcium 37(1)

      Pages: 45-56

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] DNA topoisomerase II poison TAS-103 transactivates GC-box-dependent Transcription via acetylation of Sp1.2005

    • Author(s)
      Torigoe T, Izumi H, Wakasugi T, Shibuya et al.
    • Journal Title

      Journal of Biological Chemistry 280

      Pages: 1179-1185

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Ca^<2+> clearance mechanisms in neurohypophysial terminals of the rat.2005

    • Author(s)
      Sasaki, N., Dayanithi, G, Shibuya, I.
    • Journal Title

      Cell Calcium 16

      Pages: 221-236

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] DNA topoisomerase II poison TAS-103 transactivates GC-box-dependent transcription via acetylation of Sp1.2005

    • Author(s)
      Torigoe T, Izumi H, Wakasugi T, Shibuya, I. et al.
    • Journal Title

      J.Biol.Chem. 280(2)

      Pages: 1179-1185

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Ca(2+) clearance mechanisms in neurohypophysial terminals of the rat.2005

    • Author(s)
      Sasaki N, Dayanithi G, Shibuya I
    • Journal Title

      Cell Calcium 37(1)

      Pages: 45-56

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Intracellular calcium increase and somatodendritic vasopressin release by vasopressin receptor agonists in the rat supraoptic nucleus.2004

    • Author(s)
      Sabatier N., et al.
    • Journal Title

      Journal of Neuroendocrinology 16(3)

      Pages: 221-236

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Intracellular calcium increase and somatodendritic vasopressin release by vasopressin receptor agonists in the rat supraoptic nucleus.: involvement of multiple intracellular transduction signals.2004

    • Author(s)
      Sabatier, N., Shibuya, I., Dayanithi, G.
    • Journal Title

      Journal of Neuroendocrinology 16

      Pages: 221-236

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Stimulus-secretion coupling in neurohypophysis.2004

    • Author(s)
      Shibuya, I., Dayanithi, G., Ueta, Y.
    • Journal Title

      Endocrine and Diabetes 18

      Pages: 403-410

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Intracellular calcium increase and somatodendritic vasopressin release by vasopressin receptor agonists in the rat supraoptic nucleus.2004

    • Author(s)
      Sabatier N, Shibuya I, Dayanithi G
    • Journal Title

      J.Neuroendocrinol. 16(3)

      Pages: 221-236

    • Related Report
      2004 Annual Research Report
  • [Journal Article] 下垂体後葉のStimulus-secretion coupling2004

    • Author(s)
      澁谷泉, Govindan Dayanithi, 上田陽一
    • Journal Title

      内分泌・糖尿病科 18(5)

      Pages: 403-410

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Impaired somatodendritic responses to pituitary adenylate cyclase activating polypeptide (PACAP)2003

    • Author(s)
      Jamen, F., et al.
    • Journal Title

      Journal of Neuroendocrinology 15

      Pages: 871-881

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Mechahism of cytosolic Ca^<2+> suppression by prostaglandin E_2 receptors in rat melanotr2003

    • Author(s)
      Nagata, T., et al.
    • Journal Title

      Journal of Neuroendocrinology 15

      Pages: 33-41

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] The inhibitory effects of alphaxalone on M1 and M3 muacarinic receptors expressed2003

    • Author(s)
      Shiraishi, M., et al.
    • Journal Title

      Anesthesia and analgesia 97

      Pages: 449-455

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Impaired somatodendritic responses to pituitary adenylate cyclase activating polypeptide (PACAP) of supraoptic neurones in PACAP type I -receptor deficient mice.2003

    • Author(s)
      Jamen, F., Alonso, G, Shibuya, I.et al.
    • Journal Title

      Journal of Neuroendocrinology 15

      Pages: 871-881

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Mechanisms of cytosolic Ca^<2+> suppression by prostaglandin E_2 receptors in rat melanotrophs.2003

    • Author(s)
      Nagata, T., Harayama, N., Sasaki, N., Inoue, M., Toyohira, Y, Uezono, Y., Maruyama, T., Yanagihara, N., Ueta, Y., Shibuya, I.
    • Journal Title

      Journal of Neuroendocrinology 15

      Pages: 33-41

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] The inhibitory effects of alphaxalone on M1 and M3 muscarinic receptors expressed in Xenopus oocytes.2003

    • Author(s)
      Shiraishi, M., Minami, K., Shibuya, I., Uezono, Y, Ogata, J., Okamoto, T., Murasaki, O., Kaibara, M., Ueta, Y, Shigematsu, A.
    • Journal Title

      Anesthesia and Analgesia 97

      Pages: 449-455

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Book] 内分泌・糖尿病科2003

    • Author(s)
      澁谷泉, Govindan Dayanithi, 上田陽一
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Publications] Jamen, F., Alonso, G., Shibuya, I et al.: "Impaired somatodendritic responses to pituitary adenylate cyclase-activating polypeptide (PACAP) of supraoptic neurones in PACAP type I -receptor deficient mice."Journal of Neurcendocrinology. 15. 871-881 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Nagata, T., Harayama, N., Sasaki, N.et al.: "Mechanism of cytosolic Ca^<2+> suppression by prostaglandin E_2 receptors in rat melanotrophs"Journal of Neuroendocrinology. 15. 33-41 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Shiraishi, M., Minami, K., Shibuya I.et al.: "The inhibitory effects of alphaxalone on M1 and M3 muscarinic receptors expressed in xenopus oocytes"Anesthesia and analgesia. 97. 449-455 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Sabatier, N., Shibuya, I., Dayanithi, G.: "Intracellular calcium increase and somatodendritic vasopressin release by vasopressin receptor agonists in the rat supraoptic nucleus : involvement of multiple intracellular transduction signals."Journal of Neuroendocrinology. (in press). (2004)

    • Related Report
      2003 Annual Research Report
  • [Publications] 澁谷泉, Govindan Dayanithi, 上田陽一: "下垂体後葉のStimulus-secretion coupling"内分泌・糖尿科. (印刷中). (2004)

    • Related Report
      2003 Annual Research Report

URL: 

Published: 2003-04-01   Modified: 2016-04-21  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi