| Project/Area Number |
15590216
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Environmental physiology (including Physical medicine and Nutritional physiology)
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| Research Institution | University of Occupational and Environmental Health |
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Principal Investigator |
SHIBUYA Izumi University of Occupational and Environmental Health, School of Medicine, Associate Professor, 医学部, 助教授 (50162649)
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| Co-Investigator(Kenkyū-buntansha) |
UEZONO Yasuhito Nagasaki University, School of Medicine, Assotiate Professor, 大学院・医歯薬学総合研究科, 助教授 (20213340)
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| Project Period (FY) |
2003 – 2004
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| Project Status |
Completed (Fiscal Year 2004)
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| Budget Amount *help |
¥3,500,000 (Direct Cost: ¥3,500,000)
Fiscal Year 2004: ¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 2003: ¥2,500,000 (Direct Cost: ¥2,500,000)
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| Keywords | PACAP / PACAP typeI receptor / receptor knock out mouse / supraoptic nucleus / Ca^<2+> imaging / somatodendritic release / vasopressin / oxytocin / 下垂体後葉 / 細胞内Ca^<2+> / Ca^<2+> clearance / Somatodendritic release / Signal transduction / PACAP typeI receptor / receptor knock out mouse / supraoptic nucleus / Ca^<2+> imaging / vasopressin / oxytocin |
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| Research Abstract |
The role of pituitary adenylate cyclase-activating polypeptide (PACAP) type I receptor (PAC1 receptor) in regulating hypothalamic supraoptic neurones was investigated using PAC1 receptor-deficient male mice (PAC1-/-). The effects of PACAP on [Ca2+]i were investigated in freshly dissociated supraoptic neurones and on the somatodendritic release of vasopressin and oxytocin, examined on intact supraoptic nuclei. In supraoptic neurones from wild-type mice (PAC1+/+), 100 nm PACAP induced an increase in [Ca2+]i and release of vasopressin and oxytocin, whereas in heterozygous (PAC1+/-) and null-mutant mice (PAC1-/-), PACAP was much less effective. PACAP had no effect on these two parameters when applied to isolated neurohypophysial nerve terminals of PAC l+/+ and PAC1-/- mice, and rats. In conclusion, the PAC1 receptor is solely responsible for the PACAP-induced [Ca2+]i signalling and secretion of vasopressin and oxytocin in the somatodendritic region of supraoptic neurones.
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