| Budget Amount *help |
¥3,100,000 (Direct Cost: ¥3,100,000)
Fiscal Year 2005: ¥800,000 (Direct Cost: ¥800,000)
Fiscal Year 2004: ¥900,000 (Direct Cost: ¥900,000)
Fiscal Year 2003: ¥1,400,000 (Direct Cost: ¥1,400,000)
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| Research Abstract |
To analyze functions of a novel immunoglobulin super family member (BT-IgSF;brain-and testis-specific immunoglobulin super family), we examined its expression and localization in brain and testis. When fibroblast was transfected with BT-IgSF cDNA, larger cell aggregates were formed. Neurons, cultured in dishes precoated with BT-IgSF, quickly adhered to the substratum to extend neuirtes, whereas both neuronal adhesion and protrude were abolished with antibody to BT-IgSF. In contrast, forced expression of BT-IgSF in neurons induced their detachment from surrounding cells. These data suggest that BT-IgSF may play roles in altering cellular interaction positively and negatively. In testis, round spermatid preferentially expressed BT-IgSF, while the protein molecules were detected only in sperm, indicating that BT-IgSF functions somehow in spermatogenesis. Further we are examining to see whether BT-IgSF is involved in fertilization or not. We have also examined an involvement of BT-IgSF in neuronal degeneration of cellular models for Alzheimer's disease, and found it was nothing to do with this process, although it was shown co-localized in cultured astrocytes with amyloid precursor protein that produce amyloid in Alzheimer's disease.
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