Mechanism of impairment of NO production by aging and trials for the amelioration of endothelial function
| Project/Area Number |
15590220
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
General pharmacology
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| Research Institution | Yamagata University, School of Medicine |
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Principal Investigator |
KATANO Yumi Yamagata University, School of Medicine, Professor, 医学部, 教授 (70018696)
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| Co-Investigator(Kenkyū-buntansha) |
ISHIHATA Akira Yamagata University, School of Medicine, Instructor, 医学部, 助手 (40232326)
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| Project Period (FY) |
2003 – 2004
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| Project Status |
Completed (Fiscal Year 2004)
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| Budget Amount *help |
¥3,000,000 (Direct Cost: ¥3,000,000)
Fiscal Year 2004: ¥800,000 (Direct Cost: ¥800,000)
Fiscal Year 2003: ¥2,200,000 (Direct Cost: ¥2,200,000)
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| Keywords | aging / endothelila cell / coronary circulation / aorta / red wine polyphenol / NO / PGI2 / EDHF / NOS発現量 / COX発現量 / 心筋 / 冠血管 / NO産生遊離量 / PGI_2産生遊離量 |
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| Research Abstract |
The endothelial function is impaired by aging. Red wine polyphenol compounds (RWPCs) are suggested to have anti-atherosclerotic effect by scavenging oxygen-derived free radicals. In order to elucidate the acute effect of RWPCs, we examined the response of coronary flow in Langendorff-perfused rat heart and of aortic smooth muscle to RWPCs. We have shown that RWPCs increased coronary flow in vitro in aged rat as well as young rat. RWPCs relaxed the aortic vessels concentration-dependently in phenylephrine-precontracted smooth muscle preparations, both in young and aged rats. The vasorelaxant effecs were mediated by NO, PGI2 and EDHF in young rats, while by EDHF alone in aged rats. Next, we examined the cardiovascular effect of the RWPCs orally administered for a long term (up to 3 months). Rats were divided to three groups. Normal diet, high cholesterol diet, and high cholesterol plus RWPCs diet were given to each group, respectively. After 3 months, the vascular reactivity, platelet coagulability, and response of coronary flow were examined. The long term administration of RWPCs ameliorated the cardiovascular function. In cholesterol diet group, the platelet coagulation in response to ADP was dramatically increased, but the platelet dysfunction was improved by the addition of RWPCs into cholesterol diet. The endothelila function to release nitric oxide also tended to be better in the RWPCs group than that in cholesterol group. The importance of the arginine transporters which deliver arginine as a substrate of nitric oxide into endothelilal cells will be investigated in our future study to understand the mechanism of age-related cardiovascular disorders.
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Report
(3 results)
Research Products
(24 results)
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[Journal Article] Chronic Hypertriglyceridemia in Young Watanabe Heritable Hyperlipidemic Rabbits Impaired Endothelial and Medial Smooth Muscle Function.2004
Author(s)
Tstsuro Shishido, Katsunari Tasaki, Yasuchika Takeishi, Satoshi Takasaki, Takuya Miyamato, Makoto Itoh, Hiroki Takahashi, Isao Kubota, Tsunekata Ito, Yumi Katano, Ichiro Wakabayashi, Hitonobu Tomoike
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Journal Title
Life Science 74
Pages: 1487-1501
Description
「研究成果報告書概要(欧文)」より
Related Report
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