| Project/Area Number |
15590232
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
General pharmacology
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| Research Institution | Sojo University (2005)
Kumamoto University (2003-2004) |
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Principal Investigator |
TOKUTOMI Naofumi Sojo University, Facul.Pharmac.Sci., Professor, 薬学部, 教授 (30227582)
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| Co-Investigator(Kenkyū-buntansha) |
TOKUTOMI Yoshiko Kumamoto University, Grad.Sch.Med.Sci., Instructor, 大学院・医学薬学研究部, 助手 (90253723)
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| Project Period (FY) |
2003 – 2005
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| Project Status |
Completed (Fiscal Year 2005)
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| Budget Amount *help |
¥3,100,000 (Direct Cost: ¥3,100,000)
Fiscal Year 2005: ¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 2004: ¥900,000 (Direct Cost: ¥900,000)
Fiscal Year 2003: ¥1,200,000 (Direct Cost: ¥1,200,000)
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| Keywords | neuron / Schwann cell / myelination / electromagnetic stimulation / necrosis / apoptosis |
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| Research Abstract |
Both cyclooxigenase type 1 and type 2 (COX_1 and COX_2) seem to be involved in mechanisms underlying regeneration procedure of myelinated axon of cultured dorsal root ganglion (DRG) neurons from mice when DRG neurons were gently degenerated with a field electromagnetic pulse stimulation technique. At 7 day of culture after degeneration, DRG neurons show widely spread growth of neurites with sporadic distribution of Schwann cells. We defined this stage as stage 1 (0-7 days after degeneration). From this stage, neurites of DRG neurons gradually gathered to form large bundle of myelinated neurites. We defined this stage as stage 2 (10-14 days after degeneration). SC-560, an inhibitor of COX_1 prevented formation of large bundle of myelinated neurites of DRG neurons when applied at stage 1but not NS-398, an inhibitor of COX_2. Both SC-560 and NS-398 similarly prevented formation of the large bundle of myelinated neurites when applied at stage 2. Those COX inhibitors prevented reorganization of Schwann cell distribution in addition of the effects on formation of large bundle of neurites. Those effects on regeneration procedure were additive when both drugs were simultaneously applied. These results suggest that both types of COX play important roles in regeneration procedure of myelinated DRG neurons after degeneration.
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