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An experimental model for axonal regeneration in vitro and its application

Research Project

Project/Area Number 15590232
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field General pharmacology
Research InstitutionSojo University (2005)
Kumamoto University (2003-2004)

Principal Investigator

TOKUTOMI Naofumi  Sojo University, Facul.Pharmac.Sci., Professor, 薬学部, 教授 (30227582)

Co-Investigator(Kenkyū-buntansha) TOKUTOMI Yoshiko  Kumamoto University, Grad.Sch.Med.Sci., Instructor, 大学院・医学薬学研究部, 助手 (90253723)
Project Period (FY) 2003 – 2005
Project Status Completed (Fiscal Year 2005)
Budget Amount *help
¥3,100,000 (Direct Cost: ¥3,100,000)
Fiscal Year 2005: ¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 2004: ¥900,000 (Direct Cost: ¥900,000)
Fiscal Year 2003: ¥1,200,000 (Direct Cost: ¥1,200,000)
Keywordsneuron / Schwann cell / myelination / electromagnetic stimulation / necrosis / apoptosis
Research Abstract

Both cyclooxigenase type 1 and type 2 (COX_1 and COX_2) seem to be involved in mechanisms underlying regeneration procedure of myelinated axon of cultured dorsal root ganglion (DRG) neurons from mice when DRG neurons were gently degenerated with a field electromagnetic pulse stimulation technique. At 7 day of culture after degeneration, DRG neurons show widely spread growth of neurites with sporadic distribution of Schwann cells. We defined this stage as stage 1 (0-7 days after degeneration). From this stage, neurites of DRG neurons gradually gathered to form large bundle of myelinated neurites. We defined this stage as stage 2 (10-14 days after degeneration). SC-560, an inhibitor of COX_1 prevented formation of large bundle of myelinated neurites of DRG neurons when applied at stage 1but not NS-398, an inhibitor of COX_2. Both SC-560 and NS-398 similarly prevented formation of the large bundle of myelinated neurites when applied at stage 2. Those COX inhibitors prevented reorganization of Schwann cell distribution in addition of the effects on formation of large bundle of neurites. Those effects on regeneration procedure were additive when both drugs were simultaneously applied. These results suggest that both types of COX play important roles in regeneration procedure of myelinated DRG neurons after degeneration.

Report

(4 results)
  • 2005 Annual Research Report   Final Research Report Summary
  • 2004 Annual Research Report
  • 2003 Annual Research Report
  • Research Products

    (17 results)

All 2006 2005 2004 2003 Other

All Journal Article (10 results) Patent(Industrial Property Rights) (3 results) Publications (4 results)

  • [Journal Article] Roles of ryanodine receptor in rate capability of beating ventricular myocytes in culture.2006

    • Author(s)
      Tokutomi N.
    • Journal Title

      Journal of Pharmacological Sciences 100

      Pages: 75-75

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Annual Research Report 2005 Final Research Report Summary
  • [Journal Article] Roles of ryanodine receptor in rate capability of beating ventricular myocytes in culture.2006

    • Author(s)
      Tokutomi N
    • Journal Title

      J.of Pharmacol.Sci. 100

      Pages: 75-75

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] c-kit機能欠如マウス消化管平滑筋の薬物に対する異常反応2004

    • Author(s)
      徳富 直史, 他
    • Journal Title

      日薬理誌 123・3

      Pages: 163-169

    • NAID

      10017019128

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Drug-induced anomalous contraction of gastrointestinal tract of mice with impaired c-kit function.2004

    • Author(s)
      Tokutomi N, Tokutomi Y, Nishi K
    • Journal Title

      Folia Pharmacol Jpn. 123

      Pages: 163-169

    • NAID

      10017019128

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] A distinct distribution of functional presynaptic 5-HT receptor subtypes on GABAergic nerve terminals projecting to single hippocampal CA1 pyramidal neurons2003

    • Author(s)
      Katsurabayashi S., et al.
    • Journal Title

      Neuropharmacology 44

      Pages: 1022-1030

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Effects of □_1-acid glycoproctein on isometric tension of mouse aorta2003

    • Author(s)
      Tokutomi Y., et al.
    • Journal Title

      Eur.J.Pharmacol 477・2

      Pages: 137-141

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] α_1-酸性糖淡白質の血液循環系に及ぼす作用2003

    • Author(s)
      松元 一明, 他
    • Journal Title

      人工血液 11・2

      Pages: 144-150

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] A distinct distribution of functional presynaptic 5-HT receptor subtypes on GABAergic nerve terminals projecting to single hippocampal CA1 pyramidal neurons.2003

    • Author(s)
      Katsurabayashi S, Kubota H, Tokutomi N, Akaike N
    • Journal Title

      Neuropharmacol 44

      Pages: 1022-1030

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Effects of α_1-acid glycoprotein on isometric tension of mouse aorta.2003

    • Author(s)
      Tokutomi Y, Okamoto S, Matsumoto K, Otagiri M, Nishi K, Tokutomi N
    • Journal Title

      Eur.J.Pharmacol 477・2

      Pages: 137-141

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Effects of α_1-acid glycoprotein on blood circulation.2003

    • Author(s)
      Matsumoto K, Okamoto S, Tokutomi Y, Tokutomi N, Nishi K, Maruyama T, Suenaga A, Otagiri M
    • Journal Title

      Artificial Blood 11・2

      Pages: 144-150

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Patent(Industrial Property Rights)] 2.病態ならびに生体電気信号発生培養容器2006

    • Inventor(s)
      徳富直史
    • Industrial Property Rights Holder
      科学技術振興機構他
    • Industrial Property Number
      2006-030870
    • Filing Date
      2006
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Patent(Industrial Property Rights)] 病態ならびに生体電気信号発生培養容器2006

    • Inventor(s)
      徳冨 直史
    • Industrial Property Rights Holder
      科学技術振興機構他
    • Industrial Property Number
      2006-030870
    • Filing Date
      2006-02-08
    • Related Report
      2005 Annual Research Report
  • [Patent(Industrial Property Rights)] 1.磁場又は電場刺激及びその…促進2005

    • Inventor(s)
      徳富直史
    • Industrial Property Rights Holder
      科学技術振興機構他
    • Filing Date
      2005
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Publications] 徳冨 直史, 他: "c-kit機能欠如マウス消化管平滑筋の薬物に対する異常反応"日薬理誌. 123・3. 163-169 (2004)

    • Related Report
      2003 Annual Research Report
  • [Publications] Katsurabayashi S., et al.: "A distinct distribution of functional presynaptic 5-HT receptor subtypes on GABAergic nerve terminals projecting to single hippocampal CA1 pyramidal neurons"Neuropharmacology. 44. 1022-1030 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Tokutomi Y., et al.: "Effects of α_1-acid glycoprotein on isometric tension of mouse aorta"Eur.J.Pharmacol.. 477・2. 137-141 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] 松元 一明, 他: "α_1-酸性糖蛋白質の血液循環系に及ぼす作用"人工血液. 11・2. 144-150 (2003)

    • Related Report
      2003 Annual Research Report

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Published: 2003-04-01   Modified: 2016-04-21  

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