| Budget Amount *help |
¥3,200,000 (Direct Cost: ¥3,200,000)
Fiscal Year 2004: ¥1,600,000 (Direct Cost: ¥1,600,000)
Fiscal Year 2003: ¥1,600,000 (Direct Cost: ¥1,600,000)
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| Research Abstract |
1)Antiviral study
(1)Anti-HTV assay : MT-4 cells were infected with HIV-I and incubated in the presence of various concentrations of test compounds. After incubation for 5 days, cell viability was quantified by MTT assay to estimate a inhibition activity of test compounds against HIV induced CPE. Test compounds were alkyl-ologosaccharides, synthetic peptides, and an extract from, an Indian medical plant, Indigofera cordifora.
(2)Anti-HSV assay : HSV-1 infected Vero cells were incubated in the presence of test compounds and antiviral activity was evaluated as the inhibition activity against virus induced CPE.
2)Study for anticancer activities
(1)Assay for cytotoxic activity : Three human oral tumor cell lines, (HSG, HSC-2, HSC-3), and 3 human normal cells, (HGF, HPC, HPLF) were incubated in the presence of test compounds. After 24 h incubation, the relative viable cell number was detenraned by MTT assay.
(2)Assay for DNA fragmentation : DNA fragmentation from cultured cells with test compoun
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ds treatment was determined to estimate the inhibitory activity against apoptosis.
(3)L5179 mouse T cell lymphoma cell line was transfected with MDR1/A containing retrovirus and MDR1 expressing cells were selected. The reversed activity of test compounds were studied.
(4)Caspase activation and anti-radical activity using by ESR spectroscopy were also carried out.
(5)Anti-melanoma metastatic activity was assessed using an experimental pulmonary metastasis assay of B16-BL6 melanoma cells.
According to the results of these experiments, extracts from several plants, such as Anastasia Red, demonstrate anticancer activity and inhibition of MDR expression. Tropolone derivatives have anticancer activity due to radical mediated redox reaction. 4F-benzoyl-TE14011,an analog of synthetic peptide T22 and CXCR4 antagonist, was encapsulating with a biodegradable polymer, poly D, L-lactic acid (PLA). Subcutaneous single administration of 4F-benzoyl-TE14011-PLA significantly reduced the number of colonies formed by pulmonary metastasis of B16-BL6 melanoma cells. Less
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