Prediction of CHOP therapy resistance in diffuse large B cell lymphoma: a genome-wide cDNA microarray analysis.
| Project/Area Number |
15590325
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Human pathology
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| Research Institution | Fukuoka University |
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Principal Investigator |
SUZUMIYA Junji Fukuoka University, School of Medicine, Associate Professor, 医学部, 助教授 (70206556)
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| Co-Investigator(Kenkyū-buntansha) |
OHSHIMA Koichi Fukuoka University, School of Medicine, Associate Professor, 医学部, 助教授 (50203766)
NABESHIMA Kazuki Fukuok University, School of Medicine, Associate Professor, 医学部, 助教授 (40189189)
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| Project Period (FY) |
2003 – 2004
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| Project Status |
Completed (Fiscal Year 2004)
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| Budget Amount *help |
¥3,400,000 (Direct Cost: ¥3,400,000)
Fiscal Year 2004: ¥1,600,000 (Direct Cost: ¥1,600,000)
Fiscal Year 2003: ¥1,800,000 (Direct Cost: ¥1,800,000)
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| Keywords | non-Hodgkin's lymphoma / Diffuselarge B cell lymphoma / Chemotherapy / CHOP therapy / Chemo-resistance / DNA microarray / 悪性リンパ腫 / DLBCL / cDNAマイクロアレイ / mRNA / 非ホジキンシンパ腫 / びまん性大細胞型B細胞リンパ腫 |
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| Research Abstract |
[Background and Purpose] The standard therapy is cyclophosphamide, doxorubicin, vincristine, and prednisone (CHOP) therapy for advanced diffuse large B-cell lymphoma (DLBCL) patients. About 80% patients of DLBCL reach the remission by CHOP therapy, but the remain of patients are resistant to this therapy. We studied the difference of genetic characteristics between the chemo-sensitive and chemo-resistant groups using cDNA microarray
[Patients and Methods] We selected 6 patients of CHOP-sensitive and 7 of CHOP-resistant DLBCL. We analyzed the gene expression profile of both CHOP-sensitive and CHOP-resistant groups by cDNA microarray using Cancer chip version 4.0 (Takara Bio company, Otsu, Japan).
[Results] To clarify CHOP-resistant predictor genes, we chose genes that were differentially expressed between CHOP-resistant and sensitive groups (p<0.05) in student t-test. Moreover, we selected the genes within these genes, which of CHOP-resistant group were differentiated positively or negatively more than three times of that of CHOP-sensitive group. Hierarchical clustering showed the clearly different pattern in CHOP-resistant and sensitive groups using these selected genes. There were 8 up-regulated genes included PRAME (Preferentially expressed antigen of melanoma) in CHOP-resistant group and one down-regulated gene, CD79a in CHOP-sensitive group. Furthermore, we studied the relationship, between the expression of PRAME and clinical course using RT-PCR. PRAME was positive in 23 of 77 patients with DLBCL. The survival of PRAM E positive patients was shorter than that of PRAME negative patients (p=0.0565). PRAME was positive in 12 of 45 patients having disease free survival. The prognosis of PRAME positive patients was significantly poorer than that of negative patients (p=0.037).
[Conclusion] The expression of PRAME predicts the resistance of CHOP therapy in DLBCL
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Report
(3 results)
Research Products
(52 results)
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[Journal Article] Clinicopathological features of cutaneous lesions of adult T-cell leukaemia/lymphoma.2005
Author(s)
Yamaguchi T, Ohshima K, Karube K, Tutiya T, Kawano R, Suefuji H, Shimizu A, Nakayama J, Suzumiya J, Moroi Y, Urabe K, Furue M, Koga T, Kikuchi M
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Journal Title
Br J Dermatol 152
Pages: 76-81
Description
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[Journal Article] Gene expression in adult T cellleukemia/lymphoma: up-regulation of matrix mettallopreteinase 2 in skin lesions.2004
Author(s)
Karube K, Ohshima K, Hamasaki M, Tsuchiya T, Yamaguchi T, Suefugi H, Suzumiya J, Nabeshima K, Utsunomiya A, Harada M, Kikuchi M
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Journal Title
J Clin Experiment Hematop athol 44
Pages: 67-74
Description
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[Journal Article] Expression of FoxP3, a key molecule in CD4CD25 regulatory T cells, in adult T-cell leukaemia/lymphoma cells.2004
Author(s)
Karube K, Ohshima K, Tsuchiya T, Yamaguchi T, Kawano R, Suzumiya J, Utsunomiya A, Harada M, Kikuchi M
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Journal Title
Br J Haematol 126
Pages: 81-84
Description
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[Journal Article] NK-cell Tumor Study Group. : Aggressive natural killer-cell leukemia revisited: large granular lymphocyte leukemia of cytotoxic NK cells.2004
Author(s)
Suzuki R, Suzumiya J, Nakamura S, Aoki S, Notoya A, Ozaki S, Gondo H, Hino N, Mori H, Sugimori H, Kawa K, Oshimi K
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Journal Title
Leukemia 18
Pages: 763-770
Description
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[Journal Article] Clinical results of allogeneic hemopoietic stem cell transplantation for hematological disorders.2004
Author(s)
Takamatsu Y, Kumagawa M, Suzuki K, Wakamatsu S, Ishizu M, Shirahama S, Kawano T, Shirahashi A, Shishime M, Nibu K, Suzumiya J, Tamura K
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Journal Title
Med. Bull. Fukuoka Univ. (in Japanese with English abstract) 31
Pages: 97-106
Description
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[Journal Article] Th1, Th2, and activated T-cell marker and clinical prognosis in peripheral T-cell lymphoma, unspecified: comparison with AILD, ALCL, lymphoblastic lymphoma, and ATLL.2004
Author(s)
Tsuchiya T, Ohshima K, Karube K, Yamaguchi T, Suefuji H, Hamasaki M, Kawasaki C, Suzumiya J, Tomonaga M, Kikuchi M
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[Journal Article] Differential chemokine, chemokine receptor and cytokine expression in Epstein-Barr virus- associated lymphoproliferative diseases.2003
Author(s)
Ohshima K, Karube K, Hamasaki M, Tutiya T, Yamaguchi T, Suefuji H, Suzuki K, Suzumiya J, Ohga S, Kikuchi M
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Journal Title
Leuk Lymphoma 44
Pages: 1367-1378
Description
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[Journal Article] Clinical and genetic characteristics of Japanese Burkitt lymphomas with or without leukemic presentation.2003
Author(s)
Namiki T, Sakashita A, Kobayashi H, Maseki N, Izumo T, Komada Y, Koizumi S, Shikano T, Kikuta A, Watanabe A, Suzumiya J, Kikuchi M, Kaneko Y
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Journal Title
Int J Hematol 77
Pages: 490-498
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