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Molecular analysis of etiological factors and malignant degrees for non-small cell lung cancer

Research Project

Project/Area Number 15590327
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Human pathology
Research InstitutionKanagawa Cancer Center Research Institute (2004-2005)
Research Institute for Clinical Oncology, Saitama Cancer Center (2003)

Principal Investigator

TSUCHIYA Eiju  kanagawa Cancer Center Research Institute, Director, 所長 (00072314)

Co-Investigator(Kenkyū-buntansha) HAYASHI Shinichi  Tohoku University, Medical Faculty, Professor, 医学部, 教授 (60144862)
西田 一典  埼玉がんセンター研究所, 研究室・研究員 (60280624)
Project Period (FY) 2003 – 2005
Project Status Completed (Fiscal Year 2005)
Budget Amount *help
¥3,300,000 (Direct Cost: ¥3,300,000)
Fiscal Year 2005: ¥1,100,000 (Direct Cost: ¥1,100,000)
Fiscal Year 2004: ¥1,100,000 (Direct Cost: ¥1,100,000)
Fiscal Year 2003: ¥1,100,000 (Direct Cost: ¥1,100,000)
KeywordsLung cancer / non-small cell carcinoma / Squamous cell carcinoma / Adenocarcinoma / malignancy / Carcinogenesis / p53 / k-ras / 扁平上皮癌 / K-ras / 非小細胞肺がん / p53遺伝子 / マイクロダイセクション / 非小細胞癌
Research Abstract

74 resected squamous cell carcinomas (sqs) and 239 adenocarcinomas were analyzed for p53 mutation : Any relationships between tumor location of sqs or subtype of adenocarcinomas, smoking, and p53 mutation patterns were then examined focusing on varying etiological factors. Our hypothesis was, that G→T transversion of p53 gene is caused by direct action of carcinogens contained in tobacco smoke on DNA, and that G→A transition at CpG (CpG→A) sites of the gene is caused by endogenous mechanisms. The same adenocarcinoma cases were also analyzed to determine what type of combined mutation status on p53 and k-ras affects malignancy of the tumors more strongly.
Results:1) Sqs were divided into three types by location - central, intermediate and periphera l; the highest frequencies of CpG→A in the central type, G→T in the intermediate and other mutations in the peripheral, were observed. 2)Adenocarcinomas were divided into five types - hobnail, columnar, goblet, polygonal and mixed. Of these, the hobnail type showed a low p53 mutation frequency with the highest CpG→A rate, the columnar and polygonal types had a high mutation frequency with the highest G→T rates, and the mixed type showed intermediate frequency of p53 with the highest other base change. 3)Five year survival was the highest for adenocarcinomas with the combination of non-mutated p53 and k-ras, followed by the combination of mutated p53 and non-mutated k-ras, and then the combination of non-mutated p53 and mutated k-ras, with the combination of mutated p53 and k-ras having the lowest survival.
The results show that etiological factors vary depending on locations of sqs and subtype of adenocarcinomas ; the worst malignancy was adenocarcinomas with the combined status on mutated p53 and k-ras.

Report

(4 results)
  • 2005 Annual Research Report   Final Research Report Summary
  • 2004 Annual Research Report
  • 2003 Annual Research Report
  • Research Products

    (16 results)

All 2006 2004 2003 Other

All Journal Article (14 results) Publications (2 results)

  • [Journal Article] p53 mutation spectra for squamous cell carcinomas at different levels of human bronchial branches.2006

    • Author(s)
      Shimmyo T.
    • Journal Title

      Int. J. Cancer Epub ahead of print

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] p53 mutation spectra for squamous cell carcinomas at different levels of human bronchial brances.2006

    • Author(s)
      Shimmyo T.
    • Journal Title

      Int.J.Cancer (Epub ahead of print)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] p53 mutation spectra for squamous cell carcinomas at different levels of human bronchial branches.2006

    • Author(s)
      Shimmyo T, Tsuchiya E. et al.
    • Journal Title

      Int J Cancer. (Epub ahead of print)

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Molecular markers for reinforcement of histological subclassification of neuroendocrine lung tumors.2004

    • Author(s)
      Kobayashi K.
    • Journal Title

      Cancer Sci. 95

      Pages: 334-41

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] ADAM8 as a novel serological and histochemical marker for lung cancer.2004

    • Author(s)
      Ishikawa N.
    • Journal Title

      Clin. Cancer Res. 10

      Pages: 8363-70

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Hemophagocytic syndrome associated with clostridial infection in a pancreatic carcinoma patient.2004

    • Author(s)
      Chinen K.
    • Journal Title

      Pathol. Res. Practice 20

      Pages: 241-5

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Molecular markers for reinforcement of histological subclassification of neuroendcrine lung tumors.2004

    • Author(s)
      Kobayashi K.
    • Journal Title

      Cancer Sci. 95

      Pages: 334-341

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] ADAM8 as a novel serological and histochemical marker for lung cancer.2004

    • Author(s)
      Ishikawa N.
    • Journal Title

      Clin Cancer Res. 10

      Pages: 8363-8370

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Hemophagocytic syndrome associated with clostridial infection in a pancreatic carcinoma patient.2004

    • Author(s)
      Chinen K.
    • Journal Title

      Pathol.Res.Practice 20

      Pages: 241-245

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Molecular markers for reinforcement of histological subclassification of neuroendocrine lung tumors.2004

    • Author(s)
      Kobayashi K, Tsuchiya E. et al.
    • Journal Title

      Cancer Sci. 95

      Pages: 334-341

    • Related Report
      2004 Annual Research Report
  • [Journal Article] ADAM8 as a novel serological and histochemical marker for lung cancer2004

    • Author(s)
      Ishikawa N, Tsuchiya E, Nakamura, Y, et al.
    • Journal Title

      Clin Cancer Res. 10

      Pages: 8363-8370

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Hemophagocytic syndrome associated with clostridial infection in a pancreatic carcinoma patient2004

    • Author(s)
      Chinen K, Ohkura Y, Matsubara O, Tsuchiya E
    • Journal Title

      Pathol.Res.Practice 20

      Pages: 241-245

    • Related Report
      2004 Annual Research Report
  • [Journal Article] 肺癌、病理診断に役立つ分子病理学・10、シリーズ最新医学講座・II.2004

    • Author(s)
      石川雄一, 土屋永寿
    • Journal Title

      臨床検査 48

      Pages: 1167-1172

    • Related Report
      2004 Annual Research Report
  • [Journal Article] A role for the pituitary tumor-transforming gene in the genesis and progression of non-small cell lung carcinomas.2003

    • Author(s)
      Honda S.
    • Journal Title

      Anticancer Res. 23

      Pages: 3775-3782

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Publications] Honda S, Hayashi M, Ishikawa Y, Nakagawa K, Tsuchiva E: "A role for the pituitary tumor-transforming gene in the genesis and progression of non-small cell lung carcinomas"Anticancer Res. 23. 3775-3782 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Kobayashi K, Tokuchi Y, Hashimoto T.M Tsuchiva E.et al.: "Molecular markers for reinforcement of histological subclassification of neuroendocrine lung tumors"Cancer Science. (in press).

    • Related Report
      2003 Annual Research Report

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Published: 2003-04-01   Modified: 2016-04-21  

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