| Project/Area Number |
15590345
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Experimental pathology
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| Research Institution | Ehime University |
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Principal Investigator |
KITO Katsumi EhimeUniversity, School of Medicine, Lecturer, 医学部, 講師 (00274308)
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| Co-Investigator(Kenkyū-buntansha) |
ABE Yasuhito Ehime University, School of Medicine, Associate Professor, 医学部, 助教授 (30184229)
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| Project Period (FY) |
2003 – 2004
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| Project Status |
Completed (Fiscal Year 2004)
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| Budget Amount *help |
¥3,600,000 (Direct Cost: ¥3,600,000)
Fiscal Year 2004: ¥1,400,000 (Direct Cost: ¥1,400,000)
Fiscal Year 2003: ¥2,200,000 (Direct Cost: ¥2,200,000)
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| Keywords | NUB1 / NEDD8 / AIPL1 / Proleasome |
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| Research Abstract |
NEDD8 is a ubiquitin-like protein that is involved in protein degradation by ubiquitin-proteasome system through its covalent conjugation to cullin family members. We identified NUB 1 as a novel NEDD8-interacting protein by yeast two-hybrid screening, which is induced by interferon stimulation. We produced highly purified anti-NEDD8 and anti-NUB1 polyclonal antibodies that can be used in paraffin-embedded tissue sections. Immunohistochemical study revealed that NEDD8 was widely expressed in human tissues, moreover, NEDD8 was accumulated in inclusion bodies in Parkinson's disease and Alzheimer's diseases. Recently, it has been reported that NUB I binds to AIPL1 that causes up to 12% of Leber congenital amaurosis through its mutations. AIPL1 was expressed only in the retina, however, we found that NUB1 was expressed in not only retina but also in wide variety of human tissues, especially in macrophages. In yeast two-hybrid screening for NUB1, we isolated one of the FKBP family members. Interestingly, the FKBP member is highly homologous to AIP protein, which is also similar to AIPL1. Further analysis will be needed to reveal biological functions regarding the interaction of NUB1 with the FKBP member.
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