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Regulation of apoptosis by chemotactic human ribosomal protein S19 mutant protein.

Research Project

Project/Area Number 15590350
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Experimental pathology
Research InstitutionKumamoto University

Principal Investigator

NISHIURA Hiroshi  Kumamoto University, Faculty of Medical and Pharmaceutical Sciences, Faculty Member, 大学院・医学薬学研究部, 助手 (90284760)

Co-Investigator(Kenkyū-buntansha) TANASE Sumio  Kumamoto University, School of Medicine, Professor, 医学部, 教授 (20112401)
SHIBUYA Yoko  Kumamoto University, University Hospital, Faculty Member, 医学部附属病院, 助手 (70094018)
OGAWA Minetarou  Kumamoto University, Institute of Molecular Embryology and Genetics, Professor, 発生医学センター, 教授 (70194454)
ARAKI Kimi  Kumamoto University, Institute of Molecular Embryology and Genetics, Assistant Professor, 発生医学センター, 助教授 (90211705)
Project Period (FY) 2003 – 2005
Project Status Completed (Fiscal Year 2005)
Budget Amount *help
¥3,500,000 (Direct Cost: ¥3,500,000)
Fiscal Year 2005: ¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 2004: ¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 2003: ¥1,500,000 (Direct Cost: ¥1,500,000)
KeywordsRibosomal protein S19 / mouse ES cells / Apoptosis / complement C5a receptor / Akt / ERK / 補体C5aレセプター / JAK-STAT
Research Abstract

We had a plan to find a biological effect of ribosomal protein S19 (RP S19) dimer in mouse which had an special vector with Q137N mutant RP S19 cDNA. Preliminary, established Q137N mutant-mouse NIH3T3 clones acquired to resistant apoptosis by manganese(II). From this, we suspected the ability to regulate apoptosis by RP S19 dimer-induced C5a receptor (C5aR)-mediated pathway, and reported to Joural. We started to establish mouse ES cell clones to make a mouse model. At the same time, we performed western-blotting analysis to detect a more detail mechanism about C5aR-mediated pathway, especially PI3-K/Akt and ERK pathway. We found following results. (1) Phosphorylated Akt and ERK were decreased during apoptosis. (2) Phosphorylated Akt was maintained during apoptosis in Q137N-mutant clones. (3) RP S19 dimer overcame the acquired resistance to apoptosis in Q137N mutant-clones. From these results, we strongly suggest that RP S19 dimer plays an important role in apoptosis through changing PI3-K/Akt and ERK pathway on C5aR. Now we have a final plan in vitro to discover a regulation molecule. We also still interested in making a mouse model.

Report

(4 results)
  • 2005 Annual Research Report   Final Research Report Summary
  • 2004 Annual Research Report
  • 2003 Annual Research Report
  • Research Products

    (6 results)

All 2005

All Journal Article (6 results)

  • [Journal Article] S19 ribosomal protein dimer augments metal-induced apoptosis in a mouse fibroblastic cell line by ligation of the C5a receptor.2005

    • Author(s)
      Nishiura H 他8名
    • Journal Title

      J Cell Biochem. 94

      Pages: 540-543

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Agonist and antagonist dual effect of the cross-linked S19 ribosomal protein dimer in the C5a receptor-mediated respiratory burst reaction of phagocytic leukocytes2005

    • Author(s)
      Revollo I 他5名
    • Journal Title

      Inflamm Res 54

      Pages: 82-90

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] S19 ribosomal protein dimer augments metal-induced apoptosis in a mouse fibroblastic cell line by ligation of the C5a receptor.2005

    • Author(s)
      Nishiura H, Tanase S, Shibuya Y, Futa N, Sakamoto T, Higginbottom A, Monk P, Zwirner J, Yamamoto T.
    • Journal Title

      J Cell Biochem. 94

      Pages: 540-543

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Agonist and antagonist dual effect of the cross-linked S19 ribosomal protein dimer in the C5a receptor-mediated respiratory burst reaction of phagocytic leukocytes.2005

    • Author(s)
      Revollo I, Nishiura H, Shibuya Y, Oda Y, Nishino N, Yamamoto T.
    • Journal Title

      Inflamm Res. 54

      Pages: 82-90

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Agonist and antagonist dual effect of the cross-linked S19 ribosomal protein dimmer in the C5a receptor-mediated respiratory burst reaction of phagocytic leukocytes.2005

    • Author(s)
      Hiroshi Nishiura 他5名
    • Journal Title

      Inflammation Res. 54

      Pages: 82-90

    • Related Report
      2005 Annual Research Report
  • [Journal Article] S19 Ribosomal Protein Dimer Augments Metal-Induced Apoptosis in a Mouse Fibroblastic Cell Line by Ligation of the C5a Receptor2005

    • Author(s)
      Hiroshi Nishiura 他6名
    • Journal Title

      Journal of Cellular Biochemistory 94

      Pages: 540-553

    • Related Report
      2004 Annual Research Report

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Published: 2003-04-01   Modified: 2016-04-21  

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