Functional analysis of a novel nuclear peptide H2RSP and its roles in the regeneration of injured gastrointestinal mucosa

• Project/Area Number: 15590351
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Experimental pathology
• Research Institution: University of Miyazaki (2004); 宮崎医科大学 (2003)
• Principal Investigator: ITOH Hiroshi University of Miyazaki, Faculty of Medicine, Associate Professor, 医学部, 助教授 (80253847)
• Co-Investigator(Kenkyū-buntansha): AKIYAMA Yutaka University of Miyazaki, Faculty of Medicine, Research Associate, 医学部, 助手 (00347056) ; KATAOKA Hiroaki University of Miyazaki, Faculty of Medicine, Professor, 医学部, 教授 (10214321)
• Project Period (FY): 2003 – 2004
• Project Status: Completed (Fiscal Year 2004)
• Budget Amount: ¥3,400,000 (Direct Cost: ¥3,400,000); Fiscal Year 2004: ¥1,100,000 (Direct Cost: ¥1,100,000); Fiscal Year 2003: ¥2,300,000 (Direct Cost: ¥2,300,000)
• Keywords: HGF / HGF activator inhibitor(HAI) / HAI-2-related small peptide(H2RSP) / gastrointestinal mucosa / Cell growth / Cell differentiation / in situ hybridization / immunohistochemistry / Gastreiatestinal mucisa / Cell differeatiation / In situ hybridization

Research Abstract

Hepatocyte growth factor activator inhibitor type 2(HAI-2)-related small peptide(H2RSP) is a small nuclear peptide recently identified in our laboratory. In this study, we examined the expression and cellular localization of H2RSP in normal, hyperplastic, adenomatous and carcinomatous colorectal tissues, and investigated the possible roles of H2RSP on cellular proliferation. Anti-H2RSP polyclonal antibody was raised against the recombinant human H2RSP. Immunohistochemistry and in situ hybridization were performed using surgical specimens of the patients with various gastrointestinal diseases. In normal gastrointestinal mucosae, H2RSP was immunohistochemically localized in the cytoplasm of the epithelial cells at the crypt and translocated into the nucleus of the surface epithelial cells. Its mRNA was detected mainly in the cells at the crypt by in situ hybridization. The hyperplastic epithelial cells showed almost same staining pattern as normal epithelium, while adenoma cells showed d ecreased or mixed nuclear and cytoplasmic stainig patterns. In adenocarcinomas, H2RSP was predominantly located in the cytoplasm of almost all cancer cells with various intensities, and nuclear localization was hardly visible. The level of H2RSP mRNA was apparently decreased in the cancer cells compared with corresponding normal epithelial cells by real-time RT-PCR. When cellular localization of H2RSP was examined in fractionated samples of cultured human colorectal carcinoma cells (DLD-1), H2RSP was gradually translocated from the cytoplasm into the nucleus along with the increased cellular density of DLD-1 cells in vitro. In addition, Chinese hamster ovary(CHO) cells stably transfected with H2RSP cDNA revealed the reduced cell growth in vitro. H2RSP was bound to poly(rG)-coated beads, and did not interact with any nuclear protein, single-stranded or double-stranded DNA in pull-down assay using GST-H2RSP fusion protein. The results suggest that H2RSP might be an RNA-binding protein, and have a potentially important role in the proliferation and/or differentiation of the gastrointestinal epithelial cells by translocating from the cytoplasm into the nucleus. Therefore, the impaired nuclear translocation of H2RSP may contribute to colorectal carcinogenesis.

Research Products

• [Journal Article] Diverse roles of hepatocyte growth factor activator inhibitor type 1 (HAI-1) in the growth of glioblastoma cells in vivo. (2005)
  • Author(s): Miyata S, Uchinokura S, Fukushima T, Hamasuna R, Itoh H, Akiyama Y, et al.
  • Journal Title: Cancer Lett. (in press)
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Hepatocyte growth factor activator inhibitor Type 1 (HAI-1) is required for branching morphogenesis in the chorioallantoic placenta. (2005)
  • Author(s): Tanaka H, Nagaike K, Takeda N, Itoh H, Kohama K, Fukushima T, et al.
  • Journal Title: Mol.Cell.Biol. (in press)
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Diverse roles of hepatocyte growth factor activator inhibitor type 1 (HAI-1) in the growth of glioblastoma cells in vivo. (2005)
  • Author(s): Miyata, S, Uchinokura S, Fukushima T, Hamasuna R, Itoh, H, Akiyama Y, Nakano S, Wakisaka S, Kataoka, H
  • Journal Title: Cancer Lett. (in press)
• [Journal Article] Hepatocyte growth factor activator inhibitor type 1 (HAI-1) and type 2 (HAI-2) are expressed by tubular epithelium in kidney and downregulated in renal cell carcinoma. (2004)
  • Author(s): Yamauchi M, Kataoka H, Itoh H, Seguchi T, Hasui Y, et al.
  • Journal Title: J.Urology 171 Pages: 890-896
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Regeneration of injured intestinal mucosa is impaired in hepatocyte growth factor activator-deficient mice (2004)
  • Author(s): Itoh H, Naganuma S, Takeda N, Miyata S, Uchinokura S, Fukushima T, et al.
  • Journal Title: Gastroenterology 127 Pages: 1423-1435
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Paradoxically enhanced immunoreactivity of hepatocyte growth factor activator inhibitor type 1 (HAI-1) in cancer cells at the invasion front (2004)
  • Author(s): Nagaike K, Kohama K, Uchiyama S, Tanaka H, Chijiiwa K, Itoh H, et al.
  • Journal Title: Cancer Sci. 95 Pages: 728-735
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Hepatocyte growth factor activator inhibitor type 1(HAI-1) and type 2(HAI-2) are expressed by tubular epithelium in kidney and downregulated in renal cell carcinoma. (2004)
  • Author(s): Yamauchi, M., Kataoka, H., Itoh, H., Seguchi, T., Hasui, Y., Osada, Y.
  • Journal Title: J.Urol. 171 Pages: 890-896
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Paradoxically enhanced immunoreactivity of hepatocyte growth factor activator inhibitor type 1(HAI-1) in cancer cells at the invasion front. (2004)
  • Author(s): Nagaike, K., Kohama, K., Uchiyama, S., Tanaka, H., Chijiiwa, K., Itoh, H., Kataoka, H.
  • Journal Title: Cancer Sci. 95 Pages: 728-735
  • NAID: 10014197313
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Regeneration of injured intestinal mucosa is impaired in hepatocyte growth factor activator-deficient mice. (2004)
  • Author(s): Itoh, H., Naganuma, S., Takeda, N., Miyata, S., Uchinokura, S., Fukushima, T., Uchiyama, S., Tanaka, H., Nagaike, K., Shimomura, T., Miyazawa, K., Yamada, G., Kitamura, N., Koono, M., Kataoka, H.
  • Journal Title: Gastroenterology 127 Pages: 1423-1435
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Activation of hepatocyte growth factor/scatter factor is required in colorectal carcinoma and injured intestinal mucosa. (2004)
  • Author(s): Itoh, H., Naganuma, S., Uchiyama, S., Nagaike, K., Tanaka, H., Kataoka, H.
  • Journal Title: Recent Research Developments in Biophysics and Biochemistry(Research Signpost, Kerala, India) Pages: 1-16
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Paradoxically enhanced immunoreactivity of hepatocyte growth factor activator inhibitor type 1 (HAI-1) in cancer cells at the invasion front (2004)
  • Author(s): Nagaike K, Kohama K, Uchiyama S, Tanaka H, Chijiiwa H, Itoh H, Kataoka H
  • Journal Title: Cancer Sci 95 Pages: 728-735
  • NAID: 10014197313
• [Journal Article] Regeneration of injured intestinal mucosa is impaired in hepatocyte growth factor activator-deficient mice (2004)
  • Author(s): Itoh, H, Naganuma S, Takeda N, Miyata S, Uchinokura S, Fukushima T, Uchiyama S, Tanaka H, et al.
  • Journal Title: Gastroenterology 127 Pages: 1423-1435
• [Journal Article] Characterization of transcripts generated from mouse hepatocyte growth factor activator type2 (HAI-2) and HAI-2 related small peptide (H2RSP) genes (2003)
  • Author(s): Naganuma S, Itoh H, Uchiyama S, Tanaka H, Nagaike K, et al.
  • Journal Title: Biochem.Biophys.Res.Commun. 302 Pages: 345-353
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Characterization of transcripts generated from mouse hepatocyte growth factor activator inhibitor type 2(HAI-2) and HAI-2-related small peptide(H2RSP) genes. (2003)
  • Author(s): Naganuma, S., Itoh, H., Uchiyama, S., Tanaka, H., Nagaike, K., Miyata, S., Uchinokura, S., Nuki, S., Akiyama, Y., Chijiiwa, K., Kataoka, H.
  • Journal Title: Biochem.Biophys.Res.Commun. 302 Pages: 345-353
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Diverse roles of hepatocyte growth factor activator inhibitor type 1(HAI-1) in the growth of glioblastoma cells in vivo.
  • Author(s): Miyata, S., Uchinokura, S., Fukushima, T., Hamasuna, R., Itoh, H., Akiyama, Y., Nakano, S., Wakisaka, S., Kataoka, H.
  • Journal Title: Cancer lett. (in press)
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Hepatocyte growth factor activator inhibitor type 1(HAI-1) is required for branching morphogenesis in the chorioallantonic placenta.
  • Author(s): Tanaka, H., Nagaike, K., Takeda, N., Itoh, H., Kohama, K., Fukushima, T., Miyata, S., Uchinokura, S., Shimomura, T., Miyazawa, K., Kitamura, N., Yamada, G., Kataoka, H.
  • Journal Title: Mol.Cell.Biol. (in press)
  • Description: 「研究成果報告書概要(欧文)」より
• [Book] Recent Research Development of Biophysics and Biochemistry (2004)
  • Author(s): Itoh H, Naganuma S, Uchiyama S, Nagaike K, Tanaka H, Kataoka H
  • Publisher: Research Signpost
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] Kataoka H, Tanaka H, nagaike K, Uchiyama S, Itoh H: "Roles of cancer-stroma interaction in invasive growth of cancer cells"Human Cell. 16. 1-14 (2003)
• [Publications] Naganuma S, Itoh H, Uchiyama S, Tanaka H, Nagaike K, et al.: "Characterization of transcripts generated from mouse hepatocyte growth factor activator type 2 (HAI-2) and HAI-2 related small peptide (H2RSP) genes"Biochem.Biophys.Res.Commun. 302. 345-353 (2003)
• [Publications] Yamauchi M, Kataoka H, Itoh H, Seguchi T, Hasui Y, et al.: "Hepatocyte growth factor activator inhibitor type 1 (HAI-1) and type 2 (HAI-2) are eypressed by epithelium in kidney and downregulated in renal cell carcinoma"J.Urology. 171. 890-896 (2004)