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Molecular analysis of excretory-secretory products of Trichinella causing muscle cell transformation.

Research Project

Project/Area Number 15590366
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Parasitology (including Sanitary zoology)
Research InstitutionGifu University

Principal Investigator

NAGANO Isao  Gifu University, Graduate School of Medicine, Associate Professor, 大学院・医学研究科, 助教授 (40283296)

Co-Investigator(Kenkyū-buntansha) TAKAHASHI Yuzo  Gifu University, School of Medicine, Professor, 医学部, 教授 (80094580)
WU Zhiliang  Gifu University, Graduate School of Medicine, Research Associate, 大学院・医学研究科, 助手 (90313874)
Project Period (FY) 2003 – 2004
Project Status Completed (Fiscal Year 2004)
Budget Amount *help
¥3,700,000 (Direct Cost: ¥3,700,000)
Fiscal Year 2004: ¥1,200,000 (Direct Cost: ¥1,200,000)
Fiscal Year 2003: ¥2,500,000 (Direct Cost: ¥2,500,000)
KeywordsTrichinella / Gene expression / Cyst formation / Excretory-secretory products / Enolase / Nurse cell / Microarray / apoptosis / RCD-1 / nurse cell / ミトコンドリア
Research Abstract

Infection with Trichinella spiralis or T.pseudospiralis causes muscle cell transformation and satellite cell proliferation, and T.spiralis forms typical cyst but T.pseudospiralis forms very poor cyst. We established two clones encoding the T.pseudospiralis excretory-secretory proteins related to the T.spiralis proteins, and revealed that these two proteins are important for muscle cell transformation, cyst formation and continuation of parasitism due to Trichinella.
The nurse cell in the cyst of T.spiralis comprises at least two kinds of cytoplasm, derived from muscle or satellite cells. We revealed that the nurse cell cytoplasm differed between the two Trichinella species and between the two origins of cytoplasm in the cyst of T.spiralis. A time course study was performed to reveal the sequence of histopathology after T.spiralis or T.pseudospiralis infection in mice. A cyst was formed in the former case by about 18 days post infection and prominent myopathy was restricted within the cy … More st. In the latter case, however, no typical cyst was formed, and myopathy spread diffusely over the infected muscle tissues occupying half area of muscle sections.
To search for the candidate genes responsible for nurse cell formation, cDNA microarray analysis of muscle tissues was performed before and after Trichinella infection. After the infection, several genes increased expression, and these genes after the infection responsible for cell differentiation, proliferation, cell cycle and apoptosis.
The expression of mitochondrial apoptosis related genes is elevated in Trichinella spiralis-infected muscles during encapsulation. These were expressed in the basophilic cytoplasm (infected muscle cell origin) of the nurse cells. The infected muscle cells transform but die through the process of apoptosis, which is triggered by factors from the newly formed mitochondria. The anti-apoptosis factor may help the eosinophilic cytoplasm with its survival to ensure nurse cell function. The expression of the factors of TNF-α/TNF receptor 1 signaling pathway mediating apoptosis was investigated. The infected muscle tissues up-regulate the expression of proapoptosis genes, and anti-apoptosis genes at the beginning of cyst formation. The expression returned to the normal level after cyst formation.
We established a cDNA clone from the cDNA library constructed from Trichinella spiralrs. A cDNA clone, encoded enolase that catalyzed a reversible conversion of 2-phospho-D-glyceric acid to phosphoenolpyruvate in the glycolytic pathway. Less

Report

(3 results)
  • 2004 Annual Research Report   Final Research Report Summary
  • 2003 Annual Research Report
  • Research Products

    (21 results)

All 2005 2004 2003 Other

All Journal Article (18 results) Publications (3 results)

  • [Journal Article] Tumor necrosis factor receptor-mediated apoptosis in Trichinella spiralis infected muscle cells.2005

    • Author(s)
      Zhiliang Wu
    • Journal Title

      Parasitology (in press)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] A spectrum of functional genes mobilized after Trichinella spiralis infection in skeletal muscle.2005

    • Author(s)
      Zhiliang Wu
    • Journal Title

      Parasitology (in press)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Molecular cloning and functional expression of enolase from Trichinella spiralis.2005

    • Author(s)
      Takumi Nakada
    • Journal Title

      Parasitology Research (in press)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] What is the role of p53 during the cyst formation of Trichinella spiralis? a comparable study between knockout mice and wild type mice.2005

    • Author(s)
      Thidarut Boonmars
    • Journal Title

      Parasitology (in press)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Trichinella pseudospiralis infection is characterized by more continuous and diffuse myopathy than T.spiralis infection.2005

    • Author(s)
      Thidarut Boonmars
    • Journal Title

      Parasitology Research (in press)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] A spectrum of functional genes mobilized after Trichinella spbalis infection in skeletal muscle.2005

    • Author(s)
      Zhiliang Wu
    • Journal Title

      Parasitology (in press)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Tumor necrosis factor receptor-mediated apoptosis in Trichinella spralis infected muscle cells.2005

    • Author(s)
      Zhiliang Wu
    • Journal Title

      Parasitology (in press)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Molecular cloning and functional expression of enolase from Trichinella spirails2005

    • Author(s)
      Takumi Nakada
    • Journal Title

      Parasitology Research (in press)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] What is the role of p53 during the cyst formation of Trichinella spiralls? a comparable study between knockout mice and wild type mice.2005

    • Author(s)
      Thidarut Boonmars
    • Journal Title

      Parasitology (in press)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Trichinella pseudospiralis infection is characterized by more continuous and diffuse myopathy than T. spbalis infection.2005

    • Author(s)
      Thidarut Boonmars
    • Journal Title

      Parasitology Research (in press)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Expression of apoptosis-related factors in muscles infected with Trichinella spiralis.2004

    • Author(s)
      Thidarut Boonmars
    • Journal Title

      Parasitology 128

      Pages: 323-332

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Molecular cloning and characterisation of two kinds of proteins in excretory-secretory products of Trichinella pseudospiralis.2004

    • Author(s)
      Isao Nagano
    • Journal Title

      International Journal for Parasitology 34

      Pages: 491-500

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Differences and similarities of nurse cells in cysts of Trichinella spiralis and T.pseudaspiralis.2004

    • Author(s)
      Thidarut Boonmars
    • Journal Title

      Journal of Helminthology 78(1)

      Pages: 7-16

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Expression of apoptosis related factors in muscles infected with Trichinella spiralls2004

    • Author(s)
      Thidarut Boonmars
    • Journal Title

      Parasitology 128(3)

      Pages: 323-332

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Molecular cloning and characterisation of two kinds of proteins in excretory-secretory products of Triehinella pseudaspiralis2004

    • Author(s)
      Isao Nagano
    • Journal Title

      International Journal for Parasitology 34(4)

      Pages: 491-500

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Differences and similarities of nurse cells in cysts of Trichinella spiralis and T.pseudospiralis.2003

    • Author(s)
      Thidarut Boonmars
    • Journal Title

      Journal of Helminthology 78

      Pages: 7-16

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Tumor necrosis factor receptor-mediated apoptosis in Trichinella spiralis infected muscle cells.

    • Author(s)
      Zhiliang.Wu
    • Journal Title

      Parasitology (in press)

    • Related Report
      2004 Annual Research Report
  • [Journal Article] A spectrum of functional genes mobilized after Trichinella spiralis infection in skeletal muscle.

    • Author(s)
      Zhiliang.Wu
    • Journal Title

      Parasitology (in press)

    • Related Report
      2004 Annual Research Report
  • [Publications] Boonmars, T., Wu, Z., Nagano, I., Takahashi, Y.: "Differences and similarities of nurse cells in cysts of Trichinella spiralis and T.pseudospiralis"Journal of Helminthology. (in press).

    • Related Report
      2003 Annual Research Report
  • [Publications] Boonmars, T., Wu, Z., Nagano, I., Takahashi, Y.: "Expression of apoptosis related factors in muscles infected with Trichinella spiralis"Parasitology. (in press).

    • Related Report
      2003 Annual Research Report
  • [Publications] Nagano, I., Boonmars, T., Wu, Z., Takahashi, Y.: "Molecular cloning and characterisation of two kinds of proteins in_excretory-secretory products of Thichinella pseudospiralis"International Journal for Parasitology. (in press).

    • Related Report
      2003 Annual Research Report

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Published: 2003-04-01   Modified: 2016-04-21  

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