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Population biology approach to the genome diversity of Plasmodium falciparum

Research Project

Project/Area Number 15590377
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Parasitology (including Sanitary zoology)
Research InstitutionOsaka Institute of Technology

Principal Investigator

TANABE Kazuyuki  Osaka Institute of Technology, Faulty of Engineering, Professor, 工学部, 教授 (40047410)

Co-Investigator(Kenkyū-buntansha) WAKI Seiji  Gunma Prefecture College of Health Science, Professor, 教授 (10056286)
Project Period (FY) 2003 – 2004
Project Status Completed (Fiscal Year 2004)
Budget Amount *help
¥3,600,000 (Direct Cost: ¥3,600,000)
Fiscal Year 2004: ¥1,700,000 (Direct Cost: ¥1,700,000)
Fiscal Year 2003: ¥1,900,000 (Direct Cost: ¥1,900,000)
KeywordsMalaria / Plasmodium falciparum / genome / antigen / microsatellite / genotype / Vanuatu / serca / マイクロサテライト / 遺伝子型 / 多型 / 組換え
Research Abstract

1.Genetic diversity of Plasmodiumfalciparum
Since surface antigen genes of the human malaria parasite, Plasmodium falciparum, show extensive polymorphism, a rapid evolution of their polymorphism is presumed. However, little is known about frequency of the generation of novel polymorphisms. We are interested in P.falciparum populations in Vanuatu, in the southwestern Pacific, where the malaria epidemiological settings are suitable to test whether novel antigen polymorphism evolves rapidly because infections of mixed gentoypes are rare. We analyzed single nucleotidfe polymorphisms (SNPs) and repeat-length polymorphisms in three major surface antigens, msp1,msp2 and csp, in populations from 7 islands of Vanuatu in 1996 to 2002. We also sequenced simple repeat-length polymorphisms at three microsatellite loci, serca second intron, TA4O and TA 101. Analysis of more than one million bases revealed no de novo SNPs in the three antigen genes in Vanuatu. In contrast, repeat length polymorphism e … More volved rapidly. Analysis of ‘linkage disequilibrium' between pairs of loci revealed a spectrum of population genetic structure, suggesting that some of old antigen alleles have persisted through meiotic shuffling of chromosomes after the appearance of chloroquine resistance in Vanuatu. We argue that SNPs in P.falciparum antigen genes are substantially stable in isolated populations.
2.Estimation of TMRCA of Plasmodium falciparum.
The estimation of the time to the most recent common ancestor (TMRCA) of the extant populations of Plasmodium falciparuim is crucial for understanding the genetic diversity of the parasite. To estimate TMRCA, we obtained full-length sequences of two housekeeping genes, sarcoplasmic and endoplasmic reticulum Ca^<2+>-ATPase (serca) and lactate dehydrogenase (ldh), from 11 isolates of P.falciparum and one isolate of P.reichenowi, a chimpanzee malaria parasite closely related to P.falciparum. Interspecific genetic distance between the two species and intraspecific genetic distance within P.falciparum were 0.0672±0.0088 and 0.00 11±0.0007, respectively. Based on the ratio of interspecific distance to intraspecific distance, TMRCA of P.falciparum was estimated to be (8.30±5.40) x 10^4 and (11.62±7.56) x 10^4 years ago, assuming the divergence time of the two parasite species to be 5 and 7 million years ago, respectively. Less

Report

(3 results)
  • 2004 Annual Research Report   Final Research Report Summary
  • 2003 Annual Research Report
  • Research Products

    (22 results)

All 2004 2003 Other

All Journal Article (16 results) Publications (6 results)

  • [Journal Article] Genetic distance in housekeeping genes between Plasmodium falciparum and Plasmodium reichenowi and within P.falciparum2004

    • Author(s)
      K.Tanabe, et al.
    • Journal Title

      Journal of Molecular Evolution 59

      Pages: 687-694

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Annual Research Report 2004 Final Research Report Summary
  • [Journal Article] Stable SNPs in malaria antigen genes in isolated populations2004

    • Author(s)
      Tanabe, K., Sakihama, N., A.Kaneko
    • Journal Title

      Science 303

      Pages: 493-493

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Expansion of wild type allele rather than back mutation in Pfcrt explains the recent recovery of chloroquine sensitivity of Plasmodium falciparum in Malawi2004

    • Author(s)
      T.Mita, K.Tanabe, et al.
    • Journal Title

      Molecular and Biochemical Parasitology 135

      Pages: 159-163

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Malaria dispersal among islands : human mediated Plasmodium falciparum gene flow in Vanuatu, Melanesia2004

    • Author(s)
      Lum, J.K., Tanabe, K., et al.
    • Journal Title

      Acta Tropica 90

      Pages: 181-185

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Relative frequencies of polymorphisms of variation in Block 2 repeats and 5' recombinant types of Plasmodium falciparum msp1 alleles2004

    • Author(s)
      Sakihama, N., Tanabe, K., et al.
    • Journal Title

      Parasitology International 53

      Pages: 59-67

    • NAID

      50000004910

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] A multiplex polymerase chain reaction for a differential diagnosis of Plasmodium falciparum and Plasmodium vivax.2004

    • Author(s)
      Kho, W.G., Chung, J.Y, Sim, E.J., Kim, M.Y., Kim, D.W., Jongwutiwes, S., Tanabe, K.
    • Journal Title

      Parasitology International 53

      Pages: 229-235

    • NAID

      10012543364

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Stable SNPs in malaria antigen genes in isolated populations.2004

    • Author(s)
      Tanabe, K., Sakihama, N., Kaneko, A.
    • Journal Title

      Science 303

      Pages: 493-493

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Relative frequencies of polymorphisms of variation in Block 2 repeats and 5' recombinant types of Plasmodium falciparum mspl alleles.2004

    • Author(s)
      Sakihama, N., Matsuo, T., Mitamura, T., Horii, T., Kimura, M., Kawabata, M, Tanabe, K.
    • Journal Title

      Parasitology International 53

      Pages: 59-67

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Malaria dispersal among islands : human mediated Plasmodium falciparum gene flow in Vanuatu, Melanesia.2004

    • Author(s)
      Lum, J.K., Kaneko, A., Tanabe, K., Takahashi, N., Bjorkman A, Kobayakawa T.
    • Journal Title

      Acta Tropica 90

      Pages: 181-185

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Expansion of wild type allele rather than back mutation in Pfcrt explains the recent recovery of chloroquine sensitivity of Plasmodium falciparum in Malawi.2004

    • Author(s)
      Mita, T., Kaneko, A., Lum, J.K., Zungu, I.L., Tsukahara, T., H.Eto, Kobayakawa, T.Bjorkman, A., Tanabe, K.
    • Journal Title

      Molecular and Biochemical Parasitology 135

      Pages: 159-163

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Genetic distance in housekeeping genes between Plasmodium falciparum and Plasmodium reichenowi and within P.falciparum2004

    • Author(s)
      Tanabe, K., Sakihama, N., Hattori, T., Ranford-Cartwright, L., Goldman, I., Escalante, A.A., Lal, A.A.
    • Journal Title

      Journal of Molecular Evolution 59

      Pages: 687-694

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Expansion of wild type allele rather than back mutation in Pfcrt explains the recent recovery of chloroquine sensitivity of Plasmdoium falciparum in Malawi2004

    • Author(s)
      T, Mita, K.Tanabe, et al.
    • Journal Title

      Molecular and Biochemical Parasitology 135

      Pages: 159-163

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Malaria dispersal among islands : human mediated Plasmodium falciparum gene flow in Vanuatu, Melanesia2004

    • Author(s)
      Lurn, J.K., Tanabe, K., et al.
    • Journal Title

      Acta Tropica 90

      Pages: 181-185

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Relative frequencies of polymorphisms of variation in Block 2 repeats and 5' recombinant types of Plasmodium falciparum msp1 alleles2004

    • Author(s)
      Sakiharna, N., Tanabe, K., et al.
    • Journal Title

      Parasitology International 53

      Pages: 59-67

    • NAID

      50000004910

    • Related Report
      2004 Annual Research Report
  • [Journal Article] マラリナ原虫抗原多型の進化2004

    • Author(s)
      田辺 和裄
    • Journal Title

      医学のあゆみ 209

      Pages: 107-108

    • Related Report
      2004 Annual Research Report
  • [Journal Article] A multiplex polymerase chain reaction for a differential diagnosis of Plasmodium falciparum and Plasmodium vivax2003

    • Author(s)
      Kho, W.G., Tanabe, K., et al.
    • Journal Title

      Parasitology International 52

      Pages: 229-235

    • NAID

      10012543364

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Publications] Mita, T., Tanabe, K.et al.: "Expansion of wild type allele rather than back mutation in Pfcrt explains the recent recovery of chloroquine sensitivity of Plasmdoium falciparum in Malawi"Molecular and Biochemical Parasitology. in press. (2004)

    • Related Report
      2003 Annual Research Report
  • [Publications] Tanabe, K., Sakihama, N., A.Kaneko: "Stable SNPs in malaria antigen genes in isolated populations"Science. 303. 493 (2004)

    • Related Report
      2003 Annual Research Report
  • [Publications] Lum, J.K., Tanabe, K., et al.: "Malaria dispersal among islands : human mediated Plasmodium falciparum gene flow in Vanuatu, Melanesia"Acta Tropica. in press. (2004)

    • Related Report
      2003 Annual Research Report
  • [Publications] Sakihama, N., Tanabe, K., et al.: "Relative frequencies of polymorphisms of variation in Block 2 repeats and 5' recombinant types of Plasmodium falciparum alleles"Parasitology International. in press. (2004)

    • Related Report
      2003 Annual Research Report
  • [Publications] Kho, W.G., Tanabe, K., et al.: "A multiplex polymerase chain reaction for a differential diagnosis of Plasmodium falciparum and Plasmodium vivax"Parasitology International. 52. 229-236 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Mita, T., Tanabe.K., et al.: "Recovery of chloroquine sensitivity and low prevalence of the chloroquine resistance transporter gene mutation K76T of Plasmdoium falciparum following the discontinuance of chloroquine use in Malawi"American Journal of Tropical Medicine and Hygiene. 68. 413-415 (2003)

    • Related Report
      2003 Annual Research Report

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Published: 2003-04-01   Modified: 2016-04-21  

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