| Project/Area Number |
15590378
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Parasitology (including Sanitary zoology)
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| Research Institution | Gunma University (2004)
National Institute of Infectious Diseases (2003) |
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Principal Investigator |
NOZAKI Tomoyoshi Gunma University, School of Medicine Department of Parasitology, Professor, 大学院・医学系研究科, 教授 (60198588)
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| Co-Investigator(Kenkyū-buntansha) |
SAITO Yumiko (NAKANO Yumiko) Gunma University, National Institute of Infectious Diseases Department of Parasitology, Senior Research Scientist, 寄生動物部, 主任研究官 (30321764)
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| Project Period (FY) |
2003 – 2004
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| Project Status |
Completed (Fiscal Year 2004)
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| Budget Amount *help |
¥3,600,000 (Direct Cost: ¥3,600,000)
Fiscal Year 2004: ¥1,400,000 (Direct Cost: ¥1,400,000)
Fiscal Year 2003: ¥2,200,000 (Direct Cost: ¥2,200,000)
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| Keywords | amebiasis / vesicular trafficking / Rab / retromer / protease / secretion / pathogenesis / phagocytosis / 貧食 / 病原因子 / アメーバー症 |
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| Research Abstract |
Vesicular trafficking involved in secretion of pathogenic factors and in phagocytosis is essential for virulence of Entamoeba histolytica. We identified a novel cellular compartment (prephagosomal vacuole;PPV) during erythrophagocytosis. Two Rab small GTPases Rab5 and Rab7A were shown to be essential for the formation of PPV and the transport of hydrolytic enzymes to phagosomes via PPVs. We presumed that PPV plays a role inprocessing, activation, storage, and transport of hydrolases necessary for degradation of ingested materials. We also identified a novel retromer-like complex associated with Rab7A. This complex, consisted of Vps26, Vps29, and Vps35, was shown to be involved in the regulation of the sorting of hydrolases to phagosomes. Our genomic survey of the Entamoeba genome database showed that this protist possesses at least 9 Rab7 isotypes. At least 4 isotypes Rab7A,B,D,and E showed distinct cellular distribution and were presumed to have non-redundant function. These findings on the regulation of vesicular trafficking of virulence factors should facilitate our understanding of virulence mechanisms of this important enteric protozoan parasite.
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