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Identification and characterization of Haemophilus parainfluenzae antigens associated with IgA nephropahty

Research Project

Project/Area Number 15590384
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Bacteriology (including Mycology)
Research InstitutionNagoya University

Principal Investigator

TRII Keizo  Nagoya University, Universit y Hospital, Associate Professor, 医学部附属病院, 助教授 (80324440)

Co-Investigator(Kenkyū-buntansha) OHTA Michio  Nagoya University, Graduate school of Medicine, Professor, 大学院・医学系研究科, 教授 (20111841)
Project Period (FY) 2003 – 2004
Project Status Completed (Fiscal Year 2004)
Budget Amount *help
¥3,100,000 (Direct Cost: ¥3,100,000)
Fiscal Year 2004: ¥1,200,000 (Direct Cost: ¥1,200,000)
Fiscal Year 2003: ¥1,900,000 (Direct Cost: ¥1,900,000)
KeywordsIgA nephropathy / Haemophilus parainfluenzae / mass spectrometer / IgA腎 / H.parainfluenzae
Research Abstract

Although IgA nephropathy is the most frequent nephritis that has poor prognosis in Japan, etiology of this disease is still unknown. Since the early report describing the association of IgA nephropathy and Haemophilus influenzae, we have started investigation to identify the antigen (s) of H.parainfluenzae. In previous studies, we successfully isolated the novel gene, named hpnA, as a candidate that cause IgA nephropathy. Those studies also indicated the existence of antgens other than hpnA. Therefore, we designed current experiments to find those antigens. Six strains of H.parainfluenzae isolated irom patients with IgA nephropathy were utilized. Outer membrane fractions were prepared by differential centrifugation, and separated by SDS-PAGE. For immunoblot analysis, sera from patients or healthy volunteers were used for the first antibodies, and bands only visible with patients' sera were excised from membrane. After trypsinization, samples were applied onto mass spectrometer. Proteins in samples were determined by comparison towards protein database. Results of these experiments indicated that OMP and P6 were newly identified In H.parainfluenzae, nucleotide sequence for P6 has been determined, we synthesized recombinant OMP that has histidine-tag at N-terminus, and applied it to immunoblot analysis. We found the existence of IgA antibodies against P6 in many patients with IgA nephropathy, indicating the possible involvement of P6 in the pathogenesis of this disorder.

Report

(3 results)
  • 2004 Annual Research Report   Final Research Report Summary
  • 2003 Annual Research Report
  • Research Products

    (8 results)

All 2004 2003 Other

All Journal Article (4 results) Publications (4 results)

  • [Journal Article] Two-dimensional gel electrophoresis analysis of the abundance of virulent exoproteins of group A streptococcus caused by environmental changes.2004

    • Author(s)
      Nakamura T.
    • Journal Title

      Arch Microbiol 181

      Pages: 74-81

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Characterization of Group C and G Streptococcal Strains That Cause Streptococcal Toxic Shock Syndrome.2003

    • Author(s)
      Hashikawa S.
    • Journal Title

      J Clin Microbiol 42

      Pages: 186-192

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] An unusual outbreak of infusion-related bacteremia in a gastrointestinal disease ward.2003

    • Author(s)
      Torii K.
    • Journal Title

      Jpn J Infect Dis 56

      Pages: 177-178

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Similarity of Tetracycline Resistance Genes Isolated from Fish Farm Bacteria to Those from Clinical Isolates.2003

    • Author(s)
      Furushita M.
    • Journal Title

      Appl Environ Microbiol 69

      Pages: 5336-5342

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Publications] Nakamura T, Hasegawa T, Torii K, Shimokata K, Ohta M: "Two-dimensional gel electrophoresis analysis of the abundance of virulent exoproteins of group A streptococcus caused by environmental changes"Arch Microbiol. 181. 74-81 (2004)

    • Related Report
      2003 Annual Research Report
  • [Publications] Hashikawa S, Iinuma Y, Furushita M, Torii K, Hasegawa T, Ohta M: "Characterization of Group C and G Streptococcal Strains That Cause Streptococcal Toxic Shock Syndrome"J Clin Microbiol. 42. 186-192 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Torii K, Noda Y, Miyazaki Y, Ohta M: "An unusual outbreak of infusion-related bacteremia in a gastrointestinal disease ward"Jpn J Infect Dis. 56. 177-178 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Furushita M, Kaneoka A, Takahashi Y, Torii K, Hasegawa T, Ohta M: "Similarity of Tetracycline Resistance Genes Isolated from Fish Farm Bacteria to Those from Clinical Isolates"Appl Environ Microbiol. 69. 5336-5342 (2003)

    • Related Report
      2003 Annual Research Report

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Published: 2003-04-01   Modified: 2016-04-21  

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