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Functional analysis of bacterial reverse transcriptase genes and retroelements in pathogenic bacteria

Research Project

Project/Area Number 15590388
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Bacteriology (including Mycology)
Research InstitutionHIROSHIMA UNIVERSITY

Principal Investigator

SHIMAMOTO Tadashi  Hiroshima University, Graduate School of Biosphere Science, Associate Professor, 大学院・生物圏科学研究科, 助教授 (90187443)

Co-Investigator(Kenkyū-buntansha) MIYOSHI Shin-ichi  Okayama University, Graduate School of Natural Science and Technology, Associate Professor, 大学院・自然科学研究科, 助教授 (60182060)
Project Period (FY) 2003 – 2004
Project Status Completed (Fiscal Year 2004)
Budget Amount *help
¥3,500,000 (Direct Cost: ¥3,500,000)
Fiscal Year 2004: ¥1,400,000 (Direct Cost: ¥1,400,000)
Fiscal Year 2003: ¥2,100,000 (Direct Cost: ¥2,100,000)
Keywordsreverse transcriptase / msDNA / retron / Vibrio cholerae / Salmonella Typhimurium / pathogenicity / Vibrio mimicus / コレラ菌 / Vibrio vulnificus
Research Abstract

Bacterial reverse transcriptase is responsible for the synthesis of satellite RNA-DNA complex called multicopy single-stranded DNA(msDNA). A genetic element required for the msDNA synthesis is named retron which consists of the msr-msd region, a coding region for the msDNA and the ret gene, a coding region for reverse transcriptase. Although the role(s) of retron and msDNA remains unclear, it has been reported that mutation frequency was increased in Escherichia coli under conditions in which msDNA was overproduced in a cell.
To date, we have demonstrated the existence of msDNA in pathogenic bacteria, such as Vibrio cholerae, V.parahaemolyticus, and so on, and cloned the retron regions from their chromosomes. A relationship between msDNA and/or reverse transcriptase and bacterial pathogenicity has been suggested recently. A major purpose of this study is to investigate the relationship between msDNA and/or reverse transcriptase and pathogenicity in pathogenic bacteria.
In this study, differential gene expression between wild-type V.cholerae and a ret gene mutant was examined by microarray analysis. It was found that virulence genes, such as the cholera toxin genes (ctxAB), the intestinal colonization factor gene (tcpA), ompU, and so on, were highly expressed in the ret mutant, suggesting that the msDNA and/or reverse transciptase is involved in regulation of those virulence genes in V.cholerae.
A novel msDNA, msDNA-St85, from Salmonella enterica serovar Typhimurium was isolated and characterized. It was found that the G+C content and the codon usage of retron-St85 were significantly different from those of the S.Typhimurium genome, indicating that retron-St85 was probably acquired recently in this bacterium.

Report

(3 results)
  • 2004 Annual Research Report   Final Research Report Summary
  • 2003 Annual Research Report
  • Research Products

    (25 results)

All 2005 2004 2003 Other

All Journal Article (19 results) Publications (6 results)

  • [Journal Article] A hemolysin of Vibrio mimicus (VMH) stimulates cells to produce ATP and cyclic AMP which appear to be secretory mediators2005

    • Author(s)
      Yunshan Li
    • Journal Title

      Microbiology and Immunology 49

      Pages: 73-78

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Molecular characterization of a multidrug-resistant strain of enteroinvasive Escherichia coli O164 isolated in Japan2005

    • Author(s)
      Ashraf M.Ahmed
    • Journal Title

      Journal of Medical Microbiology 54

      Pages: 273-278

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] A hemolysin of Vibrio mimicus (VMH) stimulates cells to produce ATP and cyclic AMP which appear to be secretory mediators2005

    • Author(s)
      Yunshan Li
    • Journal Title

      Microbiology and Immunology 49(1)

      Pages: 73-78

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Molecular characterization of a multidrug-resistant strain of enteroinvasive Escherichia coli O164 isolated in Japan2005

    • Author(s)
      Ashraf M.Ahmed
    • Journal Title

      Journal of Medical Microbiology 54(3)

      Pages: 273-278

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] High growing ability of Vibrio vulnificus biotype 1 is essential for production of a toxic metalloprotease causing systemic diseases in humans2004

    • Author(s)
      Hirofumi Watanabe
    • Journal Title

      Microbial Pathogenesis 36

      Pages: 117-123

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Annual Research Report 2004 Final Research Report Summary
  • [Journal Article] Distribution of virulence-associated genes in Vibrio mimicus isolates from clinical and environmental origins2004

    • Author(s)
      Sumio Shinoda
    • Journal Title

      Microbiology and Immunology 48

      Pages: 547-551

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Annual Research Report 2004 Final Research Report Summary
  • [Journal Article] Identification and characterization of class 1 integron resistance gene cassettes among Salmonella strains isolated from healthy humans in China2004

    • Author(s)
      Hongmei Zhang
    • Journal Title

      Microbiology and Immunology 48

      Pages: 639-645

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Annual Research Report 2004 Final Research Report Summary
  • [Journal Article] Regulation system for protease production in Vibrio vulnificus2004

    • Author(s)
      Tomoka Kawase
    • Journal Title

      FEMS Microbiology Letters 240

      Pages: 55-59

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Annual Research Report 2004 Final Research Report Summary
  • [Journal Article] Complex formation between a putative 66-residue thumb domain of bacterial reverse transcriptase RT-Ec86 and the primer recognition RNA2004

    • Author(s)
      Masayori Inouye
    • Journal Title

      Journal of Biological Chemistry 279

      Pages: 50735-50742

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Annual Research Report 2004 Final Research Report Summary
  • [Journal Article] Generation of active fragments from human zymogens in the bradykinin-generating cascade by extracellular proteases from Vibrio vulnificus and V. parahaemolyticus2004

    • Author(s)
      Shin-ichi Miyoshi
    • Journal Title

      Toxicon 44

      Pages: 887-893

    • NAID

      120002314106

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] High growing ability of Vibrio vulnificus biotype 1 is essential for production of a toxic metalloprotease causing systemic diseases in humans2004

    • Author(s)
      Hirofumi Watanabe
    • Journal Title

      Microbial Pathogenesis 36(3)

      Pages: 117-123

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Distribution of virulence-associated genes in Vibrio mimicus isolates from clinical and environmental origins2004

    • Author(s)
      Sumio Shinoda
    • Journal Title

      Microbiology and Immunology 48(7)

      Pages: 547-551

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Identification and characterization of class 1 integron resistance gene cassettes among Salmonella strains isolated from healthy humans in China2004

    • Author(s)
      Hongmei Zhang
    • Journal Title

      Microbiology and Immunology 48(9)

      Pages: 639-645

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Regulation system for protease production in Vibrio vulnificus2004

    • Author(s)
      Tomoka Kawase
    • Journal Title

      FEMS Microbiology Letters 240(1)

      Pages: 55-59

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Complex formation between a putative 66-residue thumb domain of bacterial reverse transcriptase RT-Ec86 and the primer recognition RNA2004

    • Author(s)
      Masayori Inouye
    • Journal Title

      Journal of Biological Chemistry 279(49)

      Pages: 50735-50742

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Generation of active fragments from human zymogens in the bradykinin-generating cascade by extracellular proteases from Vibrio vulnificus and V.parahaemolyticus2004

    • Author(s)
      Shin-ichi Miyoshi
    • Journal Title

      Toxicon 44(8)

      Pages: 887-893

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Generation of active fragments from human zymogens in the bradykinin-generating cascade by extracellular proteases from Vibrio vulnificus and V.parahaemolyticus2004

    • Author(s)
      Shin-ichi Miyoshi
    • Journal Title

      Toxicon 44

      Pages: 887-893

    • Related Report
      2004 Annual Research Report
  • [Journal Article] msDNA-St85, a multicopy single-stranded DNA isolated from Salmonella enterica serovar Typhimurium LT2 with the genomic analysis of its retron2003

    • Author(s)
      Ashraf M.Ahmed
    • Journal Title

      FEMS Microbiology Letters 224

      Pages: 291-297

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] msDNA-St85, a multicopy single-stranded DNA isolated from Salmonella enterica serovar Typhimurium LT2 with the genomic analysis of its retron2003

    • Author(s)
      Ashraf M.Ahmed
    • Journal Title

      FEMS Microbiology Letters 224(2)

      Pages: 291-297

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Publications] 島本 整: "細菌の逆転写酵素およびmsDNAの構造と機能"日本細菌学雑誌. 58. 441-445 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Ashraf M.Ahmed: "msDNA-St85, a multicopy single-stranded DNA isolated from Salmonella enterica serovar Typhimurium LT2 with the genomic analysis of its retron"FEMS Microbiology Letters. 224. 291-297 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Takashige Kashimoto: "Vibrio vulnificus induces macrophage apoptosis in vitro and in vivo"Infection and Immunity. 71. 533-535 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Shin-ichi Miyoshi: "Histamine-releasing reaction induced by the N-terminal domain of Vibrio vulnificus metalloprotease"Life Sciences. 72. 2235-2242 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] M.Jahangir Alam: "Studies on pathogenic Vibrio parahaemolyticus during a warm season in the Seto Inland Sea, Japan"Environmental Microbiology. 5. 706-710 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Tomotaka Tanabe: "Identification and characterization of genes required for biosynthesis and transport of the siderophore vibrioferrin in Vibrio parahaemolyticus"Journal of Bacteriology. 185. 6938-6949 (2003)

    • Related Report
      2003 Annual Research Report

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Published: 2003-04-01   Modified: 2016-04-21  

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