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Attempt to develop a simple and rapid method for diagnosis of cat scratch disease (Bartonella infection).

Research Project

Project/Area Number 15590393
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Bacteriology (including Mycology)
Research InstitutionKagoshima University

Principal Investigator

ODA Hiroshi  Kagoshima University, Graduate School of Medical and Dental Sciences, Professor, 大学院・医歯学総合研究科, 教授 (40107868)

Co-Investigator(Kenkyū-buntansha) YOSHIIE Kiyotaka  Kagoshima University, Graduate School of Medical and Dental Sciences, Associate Professor, 大学院・医歯学総合研究科, 助教授 (70174886)
MAENO Nobuaki  Kagoshima University, Graduate School of Medical and Dental Sciences, Research Associate, 大学院・医歯学総合研究科, 助手 (20305113)
又吉 盛健  鹿児島大学, 大学院・医歯学総合研究科, 講師 (00128456)
藤村 剛  鹿児島大学, 医学部・歯学部附属病院, 助手 (10136880)
Project Period (FY) 2003 – 2005
Project Status Completed (Fiscal Year 2005)
Budget Amount *help
¥3,600,000 (Direct Cost: ¥3,600,000)
Fiscal Year 2005: ¥800,000 (Direct Cost: ¥800,000)
Fiscal Year 2004: ¥800,000 (Direct Cost: ¥800,000)
Fiscal Year 2003: ¥2,000,000 (Direct Cost: ¥2,000,000)
Keywordscat scratch disease / emerging infectious disease / Bartonella henselae / zoonosis / SDS-PAGE / sero-diagnosis / Bartonella / western blotting / 新興・再興感染症 / western blotting / モルモット血清 / 外膜タンパク
Research Abstract

1.Analysis by SDS-PAGE of whole bacterial cells of Bartonella henselae revealed more than 35 protein bands. The molecular weight of major proteins were 90, 62, 45 (43), 40, 35, 31, and 25kDa. Molecular weight of the major outer membrane proteins were estimated as 62, 43 and 31kDa.
2.Thirty-two of 108 patients who had swelling of lymph nodes and CSD was suspected showed more than 256x IgG titer against B.henselae by indirect immunofluorescence assay (IFA). These antibody positive sera were used for the following study.
3.Western blotting analysis of whole bacterial cells of B henselae using patient sera revealed 8 bands (90, 65, 43, 40, 35, 33, 30, and 28kDa). Distribution pattern of positive bands were diverse in case to case, however, 65, 43, and 35kDa proteins showed strong antigenicity. The reactivity of IFA increased by co-cultivation of B.henselae with vero cells. This increase of reactivity were striking in 35kDa band, however, some sera did not reacted with this band. Consequently we could not find sole antigen protein useful for sero-diagnosis. Compound proteins, including 65, 43, and 35kDa protein, should be considered to develop new method for the laboratory diagnosis of CSD.
4.B.henselae stimulated the growth of lymphatic endothelial cells as well as vascular endothelial cells. B.henselae induced CCR7 on the surface of peripheral neutrophils. The bacteria also stimulated the production of CCL21 from lymphatic endothelial cells. Then we examined the diagnostic importance of CCL21 in patients with CSD. The result showed no significant difference of serum CCL21 level between CSD and other patients with various lymph node diseases.
5.We developed a new method to cultivate B.henselae, and isolated 16 strains of B.henselae from stray cats by using the method.

Report

(4 results)
  • 2005 Annual Research Report   Final Research Report Summary
  • 2004 Annual Research Report
  • 2003 Annual Research Report
  • Research Products

    (6 results)

All 2004 Other

All Journal Article (5 results) Publications (1 results)

  • [Journal Article] The induction of intercellular adhesion molecule-1 on human umbilical vein endothelial cells by a heat-stable component of Porphyromonas gingivalis.2004

    • Author(s)
      Song Mao
    • Journal Title

      Current Microbiology 48

      Pages: 108-112

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] The induction of intercellular adhesion molecule-1 on human umbilical vein endothelial cells by heat-stable component of Porphyromonas gingivalis2004

    • Author(s)
      Song Mao, et al.
    • Journal Title

      Current Microbiology 48

      Pages: 108-112

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Antiagalactosyl IgG Antibodies in juvenile idiopathic arthritis, juvenile onset Sjogren's syndrome, and healthy children.2004

    • Author(s)
      Nobuaki Maeno, Syuji Takei, Hiroshi Oda, 他6名
    • Journal Title

      Journal of Rheumatology 31

      Pages: 1211-1217

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Increased interleukin-18 expression in bone marrow of a patient with systemic juvenile idiopathic arthritis and unrecognized macrophage activation syndrome.2004

    • Author(s)
      Nobuaki Maeno, Syuji Takei, Hiroyuki Imanaka, Kimie Yamamoto, Kazumi Kuriwaki, Yoshifumi Kawano, Hiroshi Oda
    • Journal Title

      Arthritis Rheum 50

      Pages: 1935-1938

    • Related Report
      2004 Annual Research Report
  • [Journal Article] The induction of intercellular adhesion molecule-1 on human umbilical vein endothelial cells by a heat-stable component of Porphyromonas gingivalis.2004

    • Author(s)
      Song Mao, Nobuaki Maeno, Kiyotaka Yoshiie, Seiken Matayoshi, Tsuyoshi Fujimura, Hiroshi Oda
    • Journal Title

      Current Microbiology 48

      Pages: 108-112

    • Related Report
      2004 Annual Research Report
  • [Publications] Mao S (毛 松): "The induction of intercellular adhesion molecule-l on human umbilical vein endothelial cells by a heat-stable component of Porphyromonas gingivalis."Current Microbiology. 48(2). 108-112 (2004)

    • Related Report
      2003 Annual Research Report

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Published: 2003-04-01   Modified: 2016-04-21  

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