Research on molecular mechanisms of induction and suppression of neuronal death by neurovirulent viruses and on virus neuminvasiveness
| Project/Area Number |
15590424
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Virology
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| Research Institution | Aichi Medical University |
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Principal Investigator |
MORI Isamu Aichi Medical University, Department of Microbiology and Immunology, Associate Professor, 医学部, 助教授 (80283167)
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| Project Period (FY) |
2003 – 2004
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| Project Status |
Completed (Fiscal Year 2004)
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| Budget Amount *help |
¥2,700,000 (Direct Cost: ¥2,700,000)
Fiscal Year 2004: ¥1,300,000 (Direct Cost: ¥1,300,000)
Fiscal Year 2003: ¥1,400,000 (Direct Cost: ¥1,400,000)
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| Keywords | neurovirulent virus / apoptosis / JNK / p38 / olfactory system / trigeminal system / vomeronasal system / US3 / 単純ヘルペスウイルス / US11 / intercellular trafficking / 鋤鼻器官 / フェロモン / 行動 / influenza virus / herpes simplex virus / neuron / apoptosis / olfactory neurons / Fas ligand / trigeminal ganglion |
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| Research Abstract |
1 The association of virus-induced neuronal apoptosis with neuroinvasiveness was investigated in mouse systems.
Herpes simplex virus (HSV) attenuates the induction of apoptosis in peripheral nervous systems (olfactory, vomeronasal and trigeminal). But once in the brain the virus induced neuronal apoptosis. Thus, HSV likely suppresses neuronal apoptosis in the periphery to facilitate neuroinvasion into the brain.
2 The involvement of MAP kinases in neuronal apoptosis induced by viruses was investigated in mouse systems.
Infection of central neurons with neurovirulent influenza A virus (R404BP virus) resulted in phosphorylation of c-Jun N-terminal kinase (JNK) and induced apoptosis. In astrocytes, p38 MAP kinase was phosphorylated, leading to induction of inflammation including TNF production.
3 Inhibition of neuronal apoptosis by HSV accessory genes, HS3 and US11, was investigated.
Following infection with wildtype HSV, phosphorylation of JNK and resulting apoptosis were attenuated in central neurons. US3-defecient mutant induced neuronal apoptosis, suggesting that US3 protein kinase suppresses JNK activation and induction of apoptosis. US11 did not attenuate induction of apoptosis in our experimental systems. US11 showed intercellular trafficking activity in the mouse brain
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Report
(3 results)
Research Products
(24 results)
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[Journal Article] Iba1-expressing microglia respond to herpes simplex virus infection in the mouse trigeminal ganglion.2003
Author(s)
Mori I, Goshima F, Koshizuka T, Imai Y, Kohsaka S, Koide N, Sugiyama T, Yoshida T, Yokochi T, Kimura Y, Nishiyama Y.
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Journal Title
Molecular Brain Research 120
Pages: 52-56
Description
「研究成果報告書概要(欧文)」より
Related Report
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