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Study for correlation between Sarcoidosis and Propionibacteria and its application to diagnostic method

Research Project

Project/Area Number 15590489
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Laboratory medicine
Research InstitutionOkayama University

Principal Investigator

KATAOKA Mikio  Okayama University, Medical School, Professor, 医学部, 教授 (50177391)

Co-Investigator(Kenkyū-buntansha) ISHIKAWA Tetsuya  Okayama University, Medical School, Associate Professor, 医学部, 助教授 (90221754)
倉園 久生  岡山大学, 医学部, 教授 (90186487)
柴倉 美砂子  岡山大学, 医学部, 助手 (30314694)
Project Period (FY) 2003 – 2005
Project Status Completed (Fiscal Year 2005)
Budget Amount *help
¥3,500,000 (Direct Cost: ¥3,500,000)
Fiscal Year 2005: ¥800,000 (Direct Cost: ¥800,000)
Fiscal Year 2004: ¥800,000 (Direct Cost: ¥800,000)
Fiscal Year 2003: ¥1,900,000 (Direct Cost: ¥1,900,000)
KeywordsSarcoidosis / Propionibacterium acnes / Propionibacterium granulosum / Bronchoalveolar lavage / Real-time PCR / Propoinibacterium acnes / リアルタイム PCR
Research Abstract

Aim : Despite the extensive investigations into the pathogenesis of sarcoidosis, its etiology is still unknown. We have reported alveolar lymphocytes from patients with sarcoidosis are sensitized by Propionibacterium acnes (P.acnes) and the proliferation and IL-2 production in these cells were seen when these cells were stimulated by P.acnes. Recently, Eishi and his colleague have reported that many genomes of P.acnes and Propionibacterium granulosum (P.granulosum) were detected in lymph nodes from sarcoidosis patients. In this study, we investigated the quantitative analysis of Propionibacterial DNA in bronchoalveolar lavage (BAL) cells in patients with sarcoidosis to clarify the role of Propionibacteria in the pathogenesis of sarcoidosis. Moreover, we investigated the serum level of antibody.
Subjects and Methods : Bronchoalveolar lavage cells were obtained from 42 patients with sarcoidosis and from those with 30 non-sarcoid lung diseases. BAL cells were undergone assays of real-time … More PCR for Propionibacterial DNA in Light Cycler apparatus using Taq Man probes and primers to amplify 16S rRNA of Propionibacteria. DNA was extracted using QlAamp DNA Mini Kit and the concentration of DNA was measured in the spectrophotometer. The number of bacterial genomes in 500 ng of total DNA were estimated with an internal standard curve for each species. The serum level of antibody against P.acnes was estimated using whole bacterial ELISA method. The titer of antibody was shown as optical density.
Results : The number of genomes of P.acnes in BAL cells in patients with sarcoidosis are higher than in those with non-sarcoid lung diseases. However, there was no difference in the number of genomes of P.granulosum in BAL cells between sarcoidosis patients and non-sarcoidosis patients. There are clear relationship between the number of genomes of P.acnes in BAL cells and serum angiotensin converting enzyme level and proportion of macrophage and lymphocyte in BAL fluids in patients with sarcoidosis. The antibody level against P.acnes in patients with sarcoidosis was significantly higher than in those with non-sarcoid lung diseases.
Conclusion : These results suggest that P.acnes might be involved in the pathogenesis of sarcoidosis. Less

Report

(4 results)
  • 2005 Annual Research Report   Final Research Report Summary
  • 2004 Annual Research Report
  • 2003 Annual Research Report
  • Research Products

    (15 results)

All 2005 2004 2003

All Journal Article (14 results) Book (1 results)

  • [Journal Article] Sarcoidosis occurring after interferon- α therapy for chronic hepatitis C2005

    • Author(s)
      Atsushi HIRANO, Kikio KATAOKA, Yasunari NAKATA et al.
    • Journal Title

      Respirology 10

      Pages: 529-534

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Sarcoidosis occurring after interferon-α therapy for chronic hepatisis C.2005

    • Author(s)
      Atsushi Hirano
    • Journal Title

      Respirology 10

      Pages: 529-534

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Sarcoidosis occurring after interferon- α therapy for chronic hepatitis C2005

    • Author(s)
      Atsushi HIRANO, Mikio KATAOKA, Yasunari NAKATA et al.
    • Journal Title

      Respirology 10

      Pages: 529-534

    • Related Report
      2005 Annual Research Report
  • [Journal Article] 筋肉病変の自然消失後、肝病変を認めたサルコイドーシスの一例2004

    • Author(s)
      市川裕久, 片岡幹男, 尾形佳子, 中田安成他
    • Journal Title

      日本サルコイドーシス/肉芽種性疾患学会誌 24

      Pages: 71-76

    • NAID

      130004246654

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] A case of sarcoidosis manifesting liver involvement after disappearance of muscle lesion.2004

    • Author(s)
      Hirohisa Ichikawa
    • Journal Title

      The Japanese Journal of sarcoidosis and other Granulomatous disorders 24

      Pages: 71-76

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Sarcoidosis2004

    • Author(s)
      Mikio Kataoka
    • Journal Title

      Excel Nurse (Immunology and Allergology)(Medical Review Inc.)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] サルコイドーシス患者肺胞リンパ球のPropionibacterium acnesとの反応2003

    • Author(s)
      片岡幹男, 中田安成
    • Journal Title

      日本サルコイドーシス/肉芽種性疾患学会誌 23

      Pages: 23-31

    • NAID

      130004246630

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] 肺移植登録をしているサルコイドーシスの一例2003

    • Author(s)
      市川裕久, 平松順一, 片岡幹男, 中田安成他
    • Journal Title

      日本サルコイドーシス/肉芽種性疾患学会誌 23

      Pages: 71-75

    • NAID

      130004246636

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] 多彩な電動障害と不整脈のみられた心サルコイドーシスの2症例2003

    • Author(s)
      森由弘, 三好久昭, 片岡幹男, 中田安成他
    • Journal Title

      日本サルコイドーシス/肉芽種性疾患学会誌 23

      Pages: 77-82

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Propionibacterium acnes DNA detected in bronchoalveolar lavage cells from patients with sarcoidosis2003

    • Author(s)
      Hiramatsu J, Kataoka M, Nakata Y, et al.
    • Journal Title

      Sarcoidosis Vasc Deffuse Lung Dis 20・3

      Pages: 197-203

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Responsiveness of alveolar lymphocytes induced by Propionibacterium acnes in patients with sarcoidosis.2003

    • Author(s)
      Mikio Kataoka
    • Journal Title

      The Japanese Journal of sarcoidosis and other Granulomatous disorders 23

      Pages: 23-31

    • NAID

      130004246630

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] A case of sarcoidosis listed for lung transplantation.2003

    • Author(s)
      Hirohisa Ichikawa
    • Journal Title

      The Japanese Journal of sarcoidosis and other Granulomatous disorders 23

      Pages: 71-75

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Two cases of cardiac sarcoidosis with a variety of conduction disturbance and arrhythmia.2003

    • Author(s)
      Yoshihiro Mori
    • Journal Title

      The Japanese Journal of sarcoidosis and other Granulomatous disorders 23

      Pages: 77-82

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Propionibacterium acnes DNA detected in bronchoalveolar lavage cell from patients with sarcoidosis.2003

    • Author(s)
      Jun-ichi Hiramatsu
    • Journal Title

      Sarcoidosis Vasc.Diffuse Lung Dis. 20

      Pages: 197-203

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Book] サルコイドーシス.エクセルナース[免疫アレルギー編]2004

    • Author(s)
      片岡幹男
    • Total Pages
      343
    • Publisher
      メディカルレビュー社
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary

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Published: 2003-04-01   Modified: 2016-04-21  

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