| Project/Area Number |
15590553
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (C)
|
| Allocation Type | Single-year Grants |
|---|
| Section | 一般 |
|---|
| Research Field |
Public health/Health science
|
|---|
| Research Institution | Oita University (2004)
大分医科大学 (2003) |
|---|
Principal Investigator |
SHIGEMATSU Sakuji Oita University, Faculty of Medicine, Assistant Professor, 医学部, 講師 (90325704)
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
HARA Masahide Oita University, Faculty of Medicine, Assistant Professor, 医学部, 講師 (10244162)
TAKAHASHI Naohiko Oita University, Faculty of Medicine, Assistant, 医学部, 助手 (30263239)
|
|---|
| Project Period (FY) |
2003 – 2004
|
| Project Status |
Completed (Fiscal Year 2004)
|
| Budget Amount *help |
¥3,600,000 (Direct Cost: ¥3,600,000)
Fiscal Year 2004: ¥1,500,000 (Direct Cost: ¥1,500,000)
Fiscal Year 2003: ¥2,100,000 (Direct Cost: ¥2,100,000)
|
|---|
| Keywords | Resveratrol / polyphenol / oxidant stress / ischemia / reperfusion injury / vascular endothelium / leukocyte / atherosclerosis / Rho / Rho kinase |
|---|
| Research Abstract |
Moderate consumption of red wine has been suggested to exert anti-ischemic effects. Since oxidant-dependent leukocyte infiltration plays a critical role in ischemia/reperfusion-induced tissue injury, we hypothesized that resveratrol, a red wine constituent polyphenol would attenuate postischemic leukocyte recruitment and subsequent endothelial dysfunction. Intravital microscopic approaches were used to quantify leukocyte/endothelial cell interactions and venular protein leakage in rat mesenteries exposed to either 20-min ischemia and 60-min reperfusion(I/R), oxidants generated by the reaction of hypoxanthine and xanthine oxidase(HX/XO). I/R or HX/HO produced marked increases in the number of adherent(LA) and emigrated(LE) leukocytes, which were associated with significant increases in venular albumin leakage(VAL). Intravenous administration of resveratrol or superoxide dismutase(SOD) attenuated these increases in LA, LE, and VAL. Resveratrol prevents leukocyte recruitment and endothelial barrier disruption induced by I/R or HX/XO. These salutary effects appear to be related to the antioxidant properties of resveratrol and contribute to the cardioprotective actions associated with consumption of red wine.
|
