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Identification of human mitochondrial DNA based on amplification of human-specific mitochondrial sequence and its forensic application

Research Project

Project/Area Number 15590581
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Legal medicine
Research InstitutionUniversity of Miyazaki (2004)
宮崎医科大学 (2003)

Principal Investigator

YUKAWA Nobuhiro  University of Miyazaki, Faculty of Medicine, Prof., 医学部, 教授 (30240154)

Co-Investigator(Kenkyū-buntansha) MATSUDA Hirokazu  University of Miyazaki, Faculty of Medicine, Assistant Prof., 医学部, 助手 (10363582)
SEO Yasuhisa  University of Miyazaki, Faculty of Medicine, Associate Prof., 医学部, 助教授 (80187830)
KAKIZAKI Eiji  University of Miyazaki, Faculty of Medicine, Assistant Prof., 医学部, 助手 (70284833)
Project Period (FY) 2003 – 2004
Project Status Completed (Fiscal Year 2004)
Budget Amount *help
¥3,100,000 (Direct Cost: ¥3,100,000)
Fiscal Year 2004: ¥1,300,000 (Direct Cost: ¥1,300,000)
Fiscal Year 2003: ¥1,800,000 (Direct Cost: ¥1,800,000)
KeywordsForensic medicine / Human identification / Species determination / mtDNA / Cytochrome b / NADH dehydrogenase / PCR amplification
Research Abstract

Species-specific differences in a non-polymorphic region of the mitochondrial genome appear to be large enough to allow human-specific amplification of forensic DNA samples. On the other hand, sequence variation within the human mtDNA control region (hypervariable D-loop) has been widely established as a valuable marker for personal identification. We therefore developed a method of multiplex-PCR amplification of both NADH dehydrogenase subunit 4(ND4) and hypervariable D-loop region allowing species determination and personal identification. Following the multiplex-PCR amplification, amplicons are first screened on an agarose gel. The presence of double-bands indicates that the sample is human origin, while the absence of ND4 fragment indicates a non-human. Subsequent DNA sequencing of the hypervariable D-loop region allows for personal identification as the presence of ND4 fragment dose not interfere with the analysis. In addition, species and individual identification were successfully made of aged biological specimens comprising bloodstains, hair shafts and bones, illustrating the applicability of the present method to forensic specimens.

Report

(3 results)
  • 2004 Annual Research Report   Final Research Report Summary
  • 2003 Annual Research Report
  • Research Products

    (10 results)

All 2005 2004 2003 2002 Other

All Journal Article (9 results) Publications (1 results)

  • [Journal Article] Identification of DNA of human origin based on amplification of human-specific mitochondrial cytochrome b region2005

    • Author(s)
      Matsuda H, Seo Y, Kakizaki E, Kozawa S, Muraoka E, Yukawa N
    • Journal Title

      Forensic Science International (In press)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Identification of DNA of human origin based on amplification of human-specific mitochondrial cytochrome b region.2005

    • Author(s)
      Hirokazu Matsuda et al.
    • Journal Title

      Forensic Sci Int. (in press)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Identification of DNA of human origin based on amplification of human-specific mitochondrial cytochrome b region2005

    • Author(s)
      Matsuda H, Seo Y, Kakizaki E, Kozawa S, Muraoka E, Yukawa N
    • Journal Title

      Forensic Science International In press

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Multiplex-PCR法による人獣・個人同時識別法の検討2004

    • Author(s)
      松田洋和, 瀬尾泰久, 柿崎英二, 小澤周二, 村岡恵里, 湯川修弘
    • Journal Title

      日本法医学雑誌(第88次日本法医学会総会講演要旨集) 58

      Pages: 104-104

    • NAID

      120007123104

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Multiplex-PCR amplification of both NADH dehydrogenase subunit 4 and hypervariable D-loop region in mtDNA allowing species determination and personal identification.2004

    • Author(s)
      Hirokazu Matsuda et al.
    • Journal Title

      Jpn J Legal Med. 58(1)

      Pages: 104-104

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Multiplex-PCR法による人獣・個人同時識別法の検討2004

    • Author(s)
      松田洋和, 瀬尾泰久, 柿崎英二, 小澤周二, 村岡恵里, 湯川修弘
    • Journal Title

      日本法医学雑誌(第88次日本法医学会総会講演要旨集) 58・1

      Pages: 104-104

    • NAID

      120007123104

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Species identification by amplification of a Human-specific sequence in the mtDNA cytochrome b genome.2003

    • Author(s)
      Hirokazu Matsuda et al.
    • Journal Title

      Jpn J Legal Med. 57(1)

      Pages: 83-83

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] ミトコンドリアDNAチトクロムb領域のヒト特異的配列を指標とした人獣鑑別について2003

    • Author(s)
      松田洋和, 瀬尾泰久, 柿崎英二, 小澤周二, 湯川修弘
    • Journal Title

      日本法医学雑誌(第87次日本法医学会総会講演要旨集) 57・1

      Pages: 83-83

    • Related Report
      2004 Annual Research Report
  • [Journal Article] ミトコンドリアDNAチトクロムb領域のヒト特異的配列を指標とした人獣鑑別について2002

    • Author(s)
      松田洋和, 瀬尾泰久, 柿崎英二, 小澤周二, 湯川修弘
    • Journal Title

      日本法医学雑誌(第87次日本法医学会総会講演要旨集) 57

      Pages: 83-83

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Publications] 松田洋和, 湯川修弘: "ミトコンドリアDNAチトクロムb領域のヒト特異的配列を指標とした人獣鑑別について"日本法医学雑誌. 57(1). 83 (2003)

    • Related Report
      2003 Annual Research Report

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Published: 2003-04-01   Modified: 2016-04-21  

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