| Project/Area Number |
15590586
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Legal medicine
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| Research Institution | Jichi Medical School |
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Principal Investigator |
IWAMOTO Sadahiko Jichi Medical School, Dept.of Medicine, Professor, 医学部, 教授 (10232711)
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| Co-Investigator(Kenkyū-buntansha) |
KAMESAKI Toyomi Jichi Medical School, Dept.of Medicine, Lecturer, 医学部, 講師 (90316513)
OKUDA Hiroshi Jichi Medical School, Dept.of Medicine, Assos.Professor, 医学部, 助教授 (50285772)
KUMADA Maki Jichi Medical School, Dept.of Medicine, Assist.Professor, 医学部, 助手 (40326830)
OMI Toshinori Jichi Medical School, Dept.of Medicine, Assist.Professor, 医学部, 助手 (40296091)
FUKUI Emiko Utsunomiya University, Faculty of Agriculture, Assist.Professor, 農学部, 助教授 (20208341)
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| Project Period (FY) |
2003 – 2004
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| Project Status |
Completed (Fiscal Year 2004)
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| Budget Amount *help |
¥3,600,000 (Direct Cost: ¥3,600,000)
Fiscal Year 2004: ¥1,400,000 (Direct Cost: ¥1,400,000)
Fiscal Year 2003: ¥2,200,000 (Direct Cost: ¥2,200,000)
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| Keywords | ABO transgenic rat / transplantation / ABO evolution / rat ABO antigen / 移植抗原 / ラットABHオーソログ |
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| Research Abstract |
Histo-blood group ABH antigens are important not only for blood transfusion but also for organ transplantation. To improve the clinical management of transplantation, we have established transgenic rats expressing human A-transferase and B-transferase gene. Wild Wistar rats expressed A-antigen in salivary gland, intestine, and urinary bladder tissue, but B-antigen was not stained in any organs studied. A-transgenic rats expressed A-antigen in ectopic tissue of the brain plexus, type II lung epithelium, pancreas, and epidermis. B-antigen in the B-transgenic rat was expressed in the same organs as A-transgenic rats. When small intestines of wild Wistar were transplanted into A- or B-trangenic rats, the grafts were rapidly rejected compared to the grafts from A- or B-transgenic rats into wild. The systemic expression of A- or B-antigens may have modified the terminal organization of sugar chain antenna and have stimulated the innate-immune system. We have cloned authologous genes of ABO transferase from Wistar rat, which consisted of three A-transferase and one B-transferase genes. These cDNAs were mapped on rats genome draft sequence. Rat chromosome 1 accommodates B-transferase gene and chromosome 3 encodes tandem arrayed three A-transferase copies. These facts indicated that the A- and B-transferase genes were established as paralogous genes in some mammals. Primates encode single ABO gene. If the ABO ancestral genes consisted of multicopy genes with A and B transferase activity like rats, they must donate or accept gene fragments from each other and may produce a locus composed of A-alleles and B-alleles. The multicopy genes may possibly gather and bundle into a single gene through unequal cross over. This estimation must be resolved by further genome projects in other mammals.
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