| Project/Area Number |
15590593
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (C)
|
| Allocation Type | Single-year Grants |
|---|
| Section | 一般 |
|---|
| Research Field |
Legal medicine
|
|---|
| Research Institution | Kansai Medical University |
|---|
Principal Investigator |
YOSHIDA Manabu Kansai Medical University, Faculty of Medicine, Lecturer, 医学部, 講師 (20122004)
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
AKANE Atsushi Kansai Medical University, Faculty of Medicine, Professor, 医学部, 教授 (70202520)
OKII Yutaka Kansai Medical University, Faculty of Medicine, Assistant, 医学部, 助手 (20121915)
|
|---|
| Project Period (FY) |
2003 – 2005
|
| Project Status |
Completed (Fiscal Year 2005)
|
| Budget Amount *help |
¥3,000,000 (Direct Cost: ¥3,000,000)
Fiscal Year 2005: ¥600,000 (Direct Cost: ¥600,000)
Fiscal Year 2004: ¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 2003: ¥1,700,000 (Direct Cost: ¥1,700,000)
|
|---|
| Keywords | Extraction / Hydrophilic Organic Solvent / Drug / Poison / Analysis |
|---|
| Research Abstract |
Following investigation of several solvents and salts and the effect of pH to establish the new liquid-liquid extraction method using hydrophilic organic solvent, several pairs of the hydrophilic organic solvents and salts appeared suitable for the method. For liquid chromatography-mass spectrometry, ammonium acetate appeared most effective to salt out the drugs in the samples. These findings were then applied to identification and quantitation of benzodiazepines, barbiturates and antihypertensive medicines, such as barbital, phenobarbital, pentobarbital, secobarbital, nitrazepam, diazepam, bromovalerylurea, apronalide and prazosin. Furthermore, this new liquid-liquid extraction method was applied to the Triage test using gastric contents and serum specimens. The test results using samples extracted by the method was correlated better with the quantitative results by high performance liquid chromatography than those using unextracted samples.
|
