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The mechanism of liver injury by hepatitis B virus, using a novel adenovirus expressing HBV

Research Project

Project/Area Number 15590639
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Gastroenterology
Research InstitutionMie University

Principal Investigator

MURATA Kazumoto  Mie University, University Hospital, Research Associate, 医学部附属病院, 助手 (40345971)

Co-Investigator(Kenkyū-buntansha) SHIRAKI Katsuya  Mie University, University Hospital, Assistant Professor, 医学部附属病院, 講師 (90263003)
Project Period (FY) 2003 – 2004
Project Status Completed (Fiscal Year 2004)
Budget Amount *help
¥3,500,000 (Direct Cost: ¥3,500,000)
Fiscal Year 2004: ¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 2003: ¥2,500,000 (Direct Cost: ¥2,500,000)
KeywordsNKT cells / NK cells / CD1d receptor / adenovirus / B型肝炎ウイルス / アデノウイルス / マウス
Research Abstract

Five days after intravenous injection of adenovirus expressing HBV (adeno-HBV) to BALB/c mice, serum HBsAg showed a peak whereas no elevation in adeno-GFP (control virus) injected mice during follow-up. The number of intrahepatic lymphocytes increased 1 day after injection in mice injected either adeno-HBV or adeno-GFP, suggesting that this effect is due to adenovirus itself. Howevr, 5 days after injection, the number of NK cells increased again whereas no increase in adeno-GFP injected mice. The number of CD1d tertramer positive cells decreased 1 day after injection by both adenovirus. The number of CD1d tetramer positive cells recovered in a few days in adeno-GFP injected mice. In contrast, it was not recovered until 10 days after injection of adeno-HBV. NK cytotoxicity using Yac-1 cells was up-regulated 1 day after injection of both virus. Then, it gradually went back to normal in adeno-GFP injected mice whereas iNK cytotoxicity up-regulated again in adeno-HBV injected mice. From the differences on Day 5 after injection, HBV may decrease the number of CD1d tetramer positive cells and increase the number of NK cells. Injection of adeno-HBV to GD1 knockout mice demonstrated the disappearance of NK cytotoxicity. These results may indicate that HBV up-regulated NK cells through NKT cells activation.

Report

(3 results)
  • 2004 Annual Research Report   Final Research Report Summary
  • 2003 Annual Research Report
  • Research Products

    (15 results)

All 2004 2003 Other

All Journal Article (15 results)

  • [Journal Article] Tuberculous peritonitis during hemodialysis2004

    • Author(s)
      Yamamoto N
    • Journal Title

      Gastroenterology 127

      Pages: 1290-1290

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Annual Research Report 2004 Final Research Report Summary
  • [Journal Article] Tuberculous peritonitis during hemodialysis2004

    • Author(s)
      Yamamoto N, Murata K.
    • Journal Title

      Gastroenterology 127

      Pages: 1290-1290

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Hepatitis C virus-like particles combined with novel adjuvant systems enhance virus-specific immune responses.2003

    • Author(s)
      Qio M
    • Journal Title

      Hepatology 37

      Pages: 52-59

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Annual Research Report 2004 Final Research Report Summary
  • [Journal Article] Low-dose chemotherapy of cisplatin and 5-fluorouracil or doxorubicin via implanted infusion port for unresectable HCC2003

    • Author(s)
      Murata K
    • Journal Title

      Anticancer Research 23

      Pages: 1719-1722

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Immunization with hepatitis C virus-like, particles protects mice from recombinant hepatitis C virus-vaccinia infection2003

    • Author(s)
      Murata K
    • Journal Title

      Proceeding National Academy of Science of U.S.A 100

      Pages: 6753-6758

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Hepatocellular carcinoma presenting with obstructive jaundice : A clinico-pathological study of eight cases.2003

    • Author(s)
      Murata K
    • Journal Title

      Hepato-Gastroenterology 50

      Pages: 2057-2060

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Annual Research Report 2004 Final Research Report Summary
  • [Journal Article] Hepatitis C virus-like particles combined with novel adjuvant systems enhance virus-specific immune responses.2003

    • Author(s)
      Qiao M, Murata K, Davis AR, Jeong SH, Liang TJ.
    • Journal Title

      Hepatology 37

      Pages: 52-59

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Low-dose chemotherapy of cisplatin and 5-fluorouracil or doxorubicin via imported infusion port for unresectable hepatocellular carcinoma2003

    • Author(s)
      Murata K, Shiraki K, Kawakita T, Yamamoto N, Okano H, et al.
    • Journal Title

      Anticancer Res 23

      Pages: 1719-1722

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Immunization with hepatitis C virus-like particles protects mice from Recombinant hepatitis C virus-vaccinia infection2003

    • Author(s)
      Murata K, Lechmann M, Qiao M, Gunji T, Alter HJ, Liang TJ
    • Journal Title

      PNAS 100

      Pages: 6753-6758

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Hepatocellular carcinoma presenting with obstructive jaundice : A clinico-pathological study of eight cases.2003

    • Author(s)
      Murata K, Shiraki K, Kawakita T, Yamamoto N, Okano H, et al.
    • Journal Title

      Hepato-Gastroenterol 50

      Pages: 2057-2060

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Low-dose chemotherapy of cisplatin and 5-fluorouracil or doxorubic in via implanted infusion port for unresectable HCC2003

    • Author(s)
      Murata K
    • Journal Title

      Anticancer Research 23

      Pages: 1719-1722

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Immunization with hepatitis c virus-like particles protects mice from recombinant hepatitis C virus-vaccinia infection2003

    • Author(s)
      Murata K
    • Journal Title

      Proceedings National Academy of Sxience of U.S.A 100

      Pages: 6753-6758

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Macrocytic anemia during low-dose cisplatin and 5-FU through implanted infusion port for unresectable hepatobilliary malignancies

    • Author(s)
      Yamamoto N
    • Journal Title

      Anticancer Research (In press)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Macrocytic anemia during low-dose cisplatin and 5-FU through implanted Infusion port for unresectable hepatobilliary malignancies

    • Author(s)
      Yamamoto N, Murata K, Fuke H, Inoue T, Yamanaka Y, et al.
    • Journal Title

      Anticancer Res (in press)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Macrocytic anemia during low-dose cisplatin and 5-FU through implanted infusion port for unresectable hepatobilliary malignancies

    • Author(s)
      Yamamoto N
    • Journal Title

      Anticancer Research In press

    • Related Report
      2004 Annual Research Report

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Published: 2003-04-01   Modified: 2016-04-21  

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