| Project/Area Number |
15590673
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Gastroenterology
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| Research Institution | Kyoto Prefectural University of Medicine |
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Principal Investigator |
KATAOKA Keisho Kyoto Prefectural University of Medicine, Graduate School of Medical Science, Assistant Professor, 医学研究科, 助教授 (70185792)
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| Co-Investigator(Kenkyū-buntansha) |
YASUDA Hiroaki Kyoto Prefectural University of Medicine, Graduate School of Medical Science, Research Associate, 医学研究科, 助手 (40363973)
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| Project Period (FY) |
2003 – 2004
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| Project Status |
Completed (Fiscal Year 2004)
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| Budget Amount *help |
¥3,100,000 (Direct Cost: ¥3,100,000)
Fiscal Year 2004: ¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 2003: ¥2,400,000 (Direct Cost: ¥2,400,000)
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| Keywords | pancreatic progenitor cell / PDX-1 / Nestin / c-Met / BrdU / arginine / 膵腺房細胞増殖 / 分離・同定 / 免疫組織学的検討 |
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| Research Abstract |
In rat arginine-induced pancreatitis model, pancreatic acinus were degenerated and disappeared, and duct-like tubular complex was appeared on 3 days after the arginine medication. After that the proliferation of the acinar cell and the regeneration of the acinus were observed. BrdU and PCNA positive cells were scattered over the proliferating pancreatic duct cells on 3 days after the arginine medication. So, the expression of c-Met, PDX-1 and Nestin as a candidate of the cell marker for pancreatic progenitor cells which has differentiation growth capacity was examined using immunohistochemistry methods on 3 days after the arginine medication when proliferating cells appeared. An expression of c-Met was recognized in the vascular epithelial of the normal pancreas, and the significant reinforcement of expressions can not be recognized in the regenerated proliferating phase after the pancreatitis. PDX-1 was strongly expressed in the pancreatic duct cells which multiplied in the regenerated proliferating phase after the pancreatitis, though the expression in the normal pancreas was scattered in islets of Langerhans and was not recognized in the pancreatic duct cells. Nestin was expressed surrounding the multiplied pancreatic duct cells in the regenerated proliferating phase after the pancreatitis. From now on, double staining with BrdU and PDX-1 or Nestin is done, and it wants to clear the matter whether PDX-1 or a Nestin expression cell has a proliferation potency. The progenitor cells which had a differentiation potency to the acinar cells in duct-like tubular complex might exist, and PDX-1 could be thought to be useful as a marker of that progenitor cells as these results. In future, this progenitor cell is separated, and it wants to try differentiation guidance to the pancreatic acinar cell in vitro.
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