| Project/Area Number |
15590739
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Circulatory organs internal medicine
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| Research Institution | KYOTO UNIVERSITY |
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Principal Investigator |
FURUKAWA Yutaka Kyoto University, Graduate School of Medicine, Department of Cardiovascular Medicine, Assistant Professor, 医学研究科, 助手 (60359833)
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| Project Period (FY) |
2003 – 2004
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| Project Status |
Completed (Fiscal Year 2004)
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| Budget Amount *help |
¥3,500,000 (Direct Cost: ¥3,500,000)
Fiscal Year 2004: ¥1,200,000 (Direct Cost: ¥1,200,000)
Fiscal Year 2003: ¥2,300,000 (Direct Cost: ¥2,300,000)
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| Keywords | Cytokine / Suppressor of Signaling / Atherosclerosis |
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| Research Abstract |
To investigate whether enhanced expression of JAK-binding protein/suppressor of cytokine signaling 1/STAT induced STAT-inhibitor 1(JAB/SOCS-1/SSI-1) in macrophages could prevent the development of atherosclerotic lesions, JAB over-expressing apolipoprotein E-deficient mice (JAB Tg/apoE^<-/->) were generated by crossing CD11b promoter driven JAB transgenic mice (JAB Tg) and apoE^<-/-> mice.
Four JAB Tg/apoE^<-/-> and 8 JAB WT/apoE^<-/-> mice were sacrificed at 30 weeks of age and en face quantification of atherosclerotic lesions in the aorta was performed by measuring percentage of oil red-O positive area to whole aortic luminal surface area for each mouse. The percent oil red-O positive area tended to be reduced in JAB Tg/apoE^<-/-> mice (16.9±2.7^<-/-> ; mean±SD) relative to JAB WT/apoE^<-/-> mice (p=0.07).
Total serum cholesterol (T-CHO) and triglyceride levels (TG) were both comparable between the two groups : T-CHO of JAB WT/apoE^<-/-> vs. JAB Tg/apoE^<-/-> mice=530±102 mg/dL vs. 434±28 mg/dL, TG of JAB WT/apoE^<-/-> vs. JAB Tg/apoE^<-/-> mice=110±51 mg/dL vs. 95±32 mg/dL.
Thus, enhanced expression of JAB in macrophages might have beneficial effects in the inhibition of atherosclerosis. The effects appeared to be independent of lipid profile modification.
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