| Project/Area Number |
15590779
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Circulatory organs internal medicine
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| Research Institution | Kawasaki Medical School |
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Principal Investigator |
WATANABE Nozomi Kawasaki Medical School, Cardiology, Assistant Professor, 医学部, 講師 (60319960)
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| Co-Investigator(Kenkyū-buntansha) |
FUJIMOTO Katsukuni Kawasaki Medical School, Anatomy, Associate Professor, 医学部, 助教授 (80106351)
AKASAKA Takashi Kawasaki Medical School, Cardiology, Associate Professor, 医学部, 助教授 (70322584)
YOSHIDA Kiyoshi Kawasaki Medical School, Cardiology, Professor, 医学部, 教授 (60322583)
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| Project Period (FY) |
2003 – 2004
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| Project Status |
Completed (Fiscal Year 2004)
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| Budget Amount *help |
¥3,100,000 (Direct Cost: ¥3,100,000)
Fiscal Year 2004: ¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 2003: ¥2,100,000 (Direct Cost: ¥2,100,000)
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| Keywords | reperfusion injury / Nicorandil / 心筋虚血 / 再灌流障害 |
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| Research Abstract |
Background. Previous experimental and clinical studies have reported that Nicorandil, an adenosine triphosphate (ATP)-sensitive K^+ channel opener, exerts beneficial effect on microvascular function and clinical outcome after myocardial ischemia. We assessed whether intravenous administration of Nicorandil affect capillary three-dimensional (3-D) architectural characteristics and capillary volume, after early myocardial reperfusion.
Methods and Results. Using opened-chest anesthetized rats' hearts, the left anterior descending artery was occluded for 7 min followed by reperfusion. Nicorandil or saline was infused intravenously during occlusion and reperfusion. Entire coronary microvasculature was filled with contrast medium, after the hearts were arrested. Capillaries were observed 3-dimensionally, in control area and reperfused area in all samples utilizing confocal laser scanning microscopy. Capillary volume fraction was computed from 3-D images. Reperfused area in both nicorandil and saline groups showed characteristic architectural changes in capillaries. In nicorandil group, capillary volume fraction in the reperfused area was significantly reduced in saline group, compared with nicorandil group [12.7±7.2% versus 18.1±5.3% (p<0.01)].
Conclusions. Intravenous nicorandil administration beneficially affects capillary damages.
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