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Abnormalities of Cardiac Sympathetic Nerve Ending Functions in Essential Hypertension

Research Project

Project/Area Number 15590787
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Circulatory organs internal medicine
Research InstitutionNational Cardiovascular Center Research Institute

Principal Investigator

AKIYAMA Tsuyoshi  National Cardiovascular Center Research Institute, Dept.of Cardiac Physiology, Section Chief, 心臓生理部, 室長 (70202554)

Co-Investigator(Kenkyū-buntansha) YAMAZAKI Toji  National Cardiovascular Center Research Institute, Dept.of Cardiac Physiology, Section Chief, 心臓生理部, 室長 (20116122)
Project Period (FY) 2003 – 2004
Project Status Completed (Fiscal Year 2004)
Budget Amount *help
¥2,900,000 (Direct Cost: ¥2,900,000)
Fiscal Year 2004: ¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 2003: ¥1,900,000 (Direct Cost: ¥1,900,000)
Keywordshypertensive rat / sympathetic nerve endings / microdialysis technique / norepinephrine / dihydroxyphenylglycol / norepinephrine reuptke / 骨格筋交感神経終末 / ノルエピネフリン分泌 / 心臓交感神経終末 / 星状神経節刺激
Research Abstract

To elucidate whether sympathetic nerve ending functions are impaired in essential hypertension, we applied dialysis technique to the left ventricular myocardium of anesthetized SHR and WKY rats (20 weeks) and monitored myocardial interstitial norepinephrine(NE) and dihydroxyphenylglycol(DHPG) levels as indexes of NE release and intraneuronal NE metabolism on cardiac sympathetic nerve endings.
There was no difference in heart rate between SHR and WKY, but the mean arterial pressure, the weight of wet heart, plasma NE concentration, and plasma DHPG concentration in SHR were higher than those in WKY.
There was no difference in myocardial interstitial NE levels between SHR and WKY, but high K^+- and tyramine-induced interstitial NE responses in SHR were lower than those in WKY. These results suggest that NE release function on cardiac sympathetic nerve endings is impaired in SHR. There was no difference in myocardial interstitial DHPG levels and desipramine (NE reuptake inhibitor) -induced i … More nterstitial NE responses between SHR and WKY. These results suggest that NE reuptake and intraneuronal NE metabolic functions on cardiac sympathetic nerve endings are intact in SHR.
Then, we focused on skeletal muscle sympathetic nerves innervating resistant arteries as causes of high plasma NE and DHPG levels. We applied dialysis technique to the left femoral skeletal muscle of anesthetized SHR and WKY rats (20 weeks) and monitored skeletal muscle interstitial NE and DHPG levels as indexes of NE release and intraneuronal NE metabolism on skeletal muscle sympathetic nerve endings.
Skeletal muscle interstitial NE levels in SHR were higher than those in WKY. High K^+- and tyramine-induced interstitial NE responses in SHR were higher than those in WKY. These results suggest that NE release function on skeletal muscle sympathetic nerve endings is enhanced in SHR and this enhancement contributes to high plasma NE levels. Skeletal muscle interstitial DHPG levels in SHR were higher than WKY, but there was no difference in DHPG/NE ratios and desipramine-induced interstitial NE responses between SHR and WKY. These results suggest that NE reuptake and intraneuronal NE metabolic functions on skeletal muscle sympathetic nerve endings are intact in SHR. Less

Report

(3 results)
  • 2004 Annual Research Report   Final Research Report Summary
  • 2003 Annual Research Report
  • Research Products

    (26 results)

All 2004 2003 Other

All Journal Article (20 results) Publications (6 results)

  • [Journal Article] Extraneuronal enzymatic degradation of myocardial interstitial norepinephrine in the ischemic region.2004

    • Author(s)
      Fujii T, Yamazaki T, Akiyama T, Sano S, Mori H.
    • Journal Title

      Cardiovascular Research 64

      Pages: 125-131

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Effects of Ca^<2+> channel antagonists on acetylcholine and catecholamine releases in the in vivo rat adrenal medulla.2004

    • Author(s)
      Akiyama T, Yamazaki T, Mori H, Sunagawa K.
    • Journal Title

      American Journal Physiology 287

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] In vivo assessment of catechol-O-methyltransferase activity in rabbit skeletal muscle.2004

    • Author(s)
      Fujii T, Yamazaki T, Akiyama T, Sano S, Mori H.
    • Journal Title

      Autonomic Neuroscience 111

      Pages: 140-143

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Simultaneous monitoring of acetylcholine and catecholamine release in the in vivo rat adrenal medulla.2004

    • Author(s)
      Akiyama T, Yamazaki T, Mori H, Sunagawa K.
    • Journal Title

      Neurochemistry International 44

      Pages: 497-503

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Extraneuronal enzymatic degradation of myocardial interstitial norepinephrine in the ischemic region.2004

    • Author(s)
      Fujii T, Yamazaki T, Akiyama T, Sano S, Mori H.
    • Journal Title

      Cardiovasc Res 64

      Pages: 125-131

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Effects of Ca^<2+> channel antagonists on acetylcholine and catecholamine releases in the in vivo rat adrenal medulla.2004

    • Author(s)
      Akiyama T, Yamazaki T, Mori H, Sunagawa K.
    • Journal Title

      Am J Physiol 287

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] In vivo assessment of catechol-O-methyltransferase activity in rabbit skeletal muscle.2004

    • Author(s)
      Fujii T, Yamazaki T, Akiyama T, Sano S, Mori H.
    • Journal Title

      Auton Neurosci 111

      Pages: 140-143

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Simultaneous monitoring of acetylcholine and catecholamine release in the in vivo rat adrenal medulla.2004

    • Author(s)
      Akiyama T, Yamazaki T, Mori H, Sunagawa K.
    • Journal Title

      Neurochem Int 44

      Pages: 497-503

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Extraneuronal enzymatic degradation of myocardial interstitial norepinephrine in the isohemic region2004

    • Author(s)
      Fujii T, Yamazaki T, Akiyama T, Sano S, Mori H.
    • Journal Title

      Cardiovascular Research 64(1)

      Pages: 125-131

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Effects of Ca^<2+> channel antagonists on acetylcholine and catecholamine releases in the in vivo rat adrenal medulla.2004

    • Author(s)
      Akiyama T, Yamazaki T, Mori H, Sunagawa K.
    • Journal Title

      American Journal Physiology 287(1)

    • Related Report
      2004 Annual Research Report
  • [Journal Article] In vivo assessment of catechol-O-methyltransferase activity in rabbit skeletal muscle.2004

    • Author(s)
      Fujii T, Yamazaki T, Akiyama T, Sano S, Mori H.
    • Journal Title

      Autonomic Neuroscience 111(2)

      Pages: 140-143

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Inhibition of cholinesterase elicits muscarinic receptor-mediated synaptic transmission in the rat adrenal medulla.2003

    • Author(s)
      Akiyama T, Yamazaki T, Mori H, Sunagawa K
    • Journal Title

      Autonomic Neuroscience 107

      Pages: 65-73

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Acute limb ischemia does not facilitate but inhibits norepinephrine release from muscle sympathetic nerve endings in anesthetized rabbit.2003

    • Author(s)
      Tokunaga N, Yamazaki T, Akiyama T, Sano S, Mori H.
    • Journal Title

      J Cardiovasc Pharmacol 42(Suppl 1)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] N^G-nitro-L-arginine methyl ester-induced norepinephrine release from cardiac sympathetic nerve endings in anesthetized cats.2003

    • Author(s)
      Takauchi Y, Yamazaki T, Akiyama T, Sunagawa K.
    • Journal Title

      Neurosci Lett 353

      Pages: 205-208

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Detection of 3-methoxy-4-hydroxyphenylglycol in rabbit skeletal muscle microdialysate.2003

    • Author(s)
      Tokunaga N, Yamazaki T, Akiyama T, Mori H.
    • Journal Title

      J Chromatogr B Analyt Technol Biomed Life Sci 798

      Pages: 163-166

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Inhibition of cholinesterase elicits muscarinic receptor-mediated synaptic transmission in the rat adrenal medulla.2003

    • Author(s)
      Akiyama T, Yamazaki T, Mori H, Sunagawa K.
    • Journal Title

      Auton Neurosci 107

      Pages: 65-73

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] In vivo monitoring of norepinephrine and its metabolites in skeletal muscle.2003

    • Author(s)
      Tokunaga N, Yamazaki T, Akiyama T, Sano S, Mori H.
    • Journal Title

      Neurochem Int 43

      Pages: 573-580

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Acute limb ischemia does not facilitate but inhibits norepinephrine release from muscle sympathetic nerve endings in anesthetized rabbit.2003

    • Author(s)
      Tokunaga N, Yamazaki T, Akiyama T, Sano S, Mori H.
    • Journal Title

      Journal of Cardiovascular Pharmacology 42(Suppl 1)

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Microdialysis separately monitors myocardial interstitial myoglobin during ischemic and reperfusion periods.

    • Author(s)
      Kitagawa H, Yamazaki T, Akiyama T, Sugimachi M, Sunagawa K, Mori H.
    • Journal Title

      American Journal Physiology (in press)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Microdialysis separately monitors myocardial interstitial myoglobin during ischemic and reperfusion periods.

    • Author(s)
      Kitagawa H, Yamazaki T, Akiyama T, Sugimachi M, Sunagawa K, Mori H.
    • Journal Title

      Am J Physiol (in press)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Publications] Tsuyoshi Akiyama: "Inhibition of cholinesterase elicits muscarinic receptor-mediated synaptic transmission in the rat adrenal medulla."Auton Neurosci. 107. 65-73 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Tsuyoshi Akiyama: "Simultaneous monitoring of acetylcholine and catecholamine release in the in vivo rat adrenal medulla."Neurochem Int. 44. 497-503 (2004)

    • Related Report
      2003 Annual Research Report
  • [Publications] Tsuyoshi Akiyama: "Effects of Ca^<2+> channel antagonists on acetylcholine and catecholamine releases in the in vivo rat adrenal medulla."Am J Physiol Regul Integr Comp Physiol. (in press).

    • Related Report
      2003 Annual Research Report
  • [Publications] Yuji Takauchi: "NG-nitro-L-arginine methyl ester-induced norepinephrine release from cardiac sympathetic nerve endings in anesthetized cats."Neurosci Lett. 353. 205-208 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Noriyuki Tokunaga: "Detection of 3-methoxy-4-hydroxyphenylglycol in rabbit skeletal muscle microdialysate."J Chromatogr B Analyt Technol Biomed Life Sci. 798. 163-166 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Noriyuki Tokunaga: "In vivo monitoring of norepinephrine and its metabolites in skeletal muscle."Neurochem Int. 43. 573-580 (2003)

    • Related Report
      2003 Annual Research Report

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Published: 2003-04-01   Modified: 2016-04-21  

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