| Project/Area Number |
15590803
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Respiratory organ internal medicine
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| Research Institution | Hamamatsu University School of Medicine |
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Principal Investigator |
SUDA Takafumi Hamamatsu University School of Medicine, 2^<nd> Division of Internal Medicine, Assistant Professor, 医学部, 助手 (30291397)
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| Co-Investigator(Kenkyū-buntansha) |
CHIDA Kingo Hamamatsu University School of Medicine, 2^<nd> Division of Internal Medicine, Associate Professor, 医学部, 助教授 (40197611)
NAGATA Toshi Hamamatsu University School of Medicine, Department of microbiology and Immunology, Associate Professor, 医学部, 助教授 (90275024)
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| Project Period (FY) |
2003 – 2004
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| Project Status |
Completed (Fiscal Year 2004)
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| Budget Amount *help |
¥3,300,000 (Direct Cost: ¥3,300,000)
Fiscal Year 2004: ¥1,400,000 (Direct Cost: ¥1,400,000)
Fiscal Year 2003: ¥1,900,000 (Direct Cost: ¥1,900,000)
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| Keywords | Dendritic cell / vaccine / tuberculosis |
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| Research Abstract |
In the present study, we evaluated antigen 85A (Ag85A)-transduced dendritic cells (DCs) vaccine against Mycobacterium tuberculosis. Murine bone marrow-derived DCs were retrovirally transduced with mycobacterial Ag85A and injected to BALB/c mice intravenously. The DCs vaccine was capable of generating purified protein derivative (PPD)-, and the antigen-specific spleen cell proliferation and IFN-gamma production from the spleen cells. In addition, the DC vaccination induced cytotoxic T-lymphocytes (CTL) exhibiting lytic activity and IFN-gamma production specific to a CTL epitope on Ag85A molecule. This eliciting cellular immunity led to induction of moderate protective immunity against Mycobacterium tuberculosis
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