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The mechanisms of highly metastetic capasity in highly metastatic subpopulations of lung adenocarcinoma cell line and these clinical applications

Research Project

Project/Area Number 15590831
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Respiratory organ internal medicine
Research InstitutionNippon Medical School

Principal Investigator

GEMMA Akihiko  NIPPON MEDICAL SCHOOL, 4^<TH> DEPT OF INTERNAL MED, ASSOCIATE PROF., 医学部, 助教授 (20234651)

Co-Investigator(Kenkyū-buntansha) KUDOH Shoji  NIPPON MEDICAL SCHOOL, GRADUATE COURSE, PULMONARY MEDICINE/INFECTION AND ONCOLOGY, PROF., 大学院・医学研究科, 教授 (40256912)
植松 和嗣  東海大学, 医学部, 助手 (10297880)
Project Period (FY) 2003 – 2004
Project Status Completed (Fiscal Year 2004)
Budget Amount *help
¥3,500,000 (Direct Cost: ¥3,500,000)
Fiscal Year 2004: ¥1,500,000 (Direct Cost: ¥1,500,000)
Fiscal Year 2003: ¥2,000,000 (Direct Cost: ¥2,000,000)
KeywordsLUNG CANCER / METASTASIS / PROTEOME / cDNA array / Galectin-1 / SiRNA / 2D-DIGE / CGH array
Research Abstract

To identify novel key factors of tumor metastasis in lung cancer, we established a highly metastatic human lung adenocarcinoma cell line in an experimental metastasis model by repeatedly inoculating the cells into nude mice and, subsequently, culturing tumor cells harvested from the pulmonary metastatic foci and we established the gene expression profiles of two adenocarcinoma cell line variants by use of genome-wide human cDNA microarray analysis and comparing these profiles with that of the parental cell line. We identified novel genes in the highly metastatic cell lines that showed a significantly enhanced or reduced expression. Our analysis in subpopulations of a lung cancer cell line indicated that the highly metastatic potential of lung cancer may be induced not by an alteration in the expression of a single gene, but by the accumulation of alterations in the expression of several genes. In this study, we used proteome analysis (2D-DIGE, Ab-array) and newly-developed cDNA array and identified 82 novel candidates including galectin-1. We are evaluating these genes as key molecules involved in metastasis in lung cancer ; (1)confirm these expressions by real-time RT-PCR, Northern blot, and Western blot, (2)confirm these function by SiRNA et al., (3)evaluate clinical utility of these molecules by immunohistochemistry in clinical specimens.

Report

(3 results)
  • 2004 Annual Research Report   Final Research Report Summary
  • 2003 Annual Research Report
  • Research Products

    (24 results)

All 2004 2003 Other

All Journal Article (20 results) Publications (4 results)

  • [Journal Article] Proteomic signature of human cancer cells.2004

    • Author(s)
      Seike M
    • Journal Title

      Proteomics 4(9)

      Pages: 2776-2788

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Annual Research Report 2004 Final Research Report Summary
  • [Journal Article] Mutational analysis of the macrophage scavenger receptor 1 (MSR1) gene in primary lung cancer2004

    • Author(s)
      Yoshimura A
    • Journal Title

      J Nippon Med Sch 71(2)

      Pages: 99-104

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Annual Research Report 2004 Final Research Report Summary
  • [Journal Article] The difference of angiogenesis in human lung adenocarcinoma cell lines with different metastatic potency2004

    • Author(s)
      Zou D
    • Journal Title

      J Nippon Med Sch 71(3)

      Pages: 181-189

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Annual Research Report 2004 Final Research Report Summary
  • [Journal Article] Proteomic signature of human cancer cells.2004

    • Author(s)
      Seike M
    • Journal Title

      Proteomics. 4(9)

      Pages: 2776-2788

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Mutational analysis of the macrophage scavenger receptor 1(MSR1) gene in primary lung cancer.2004

    • Author(s)
      Yoshimura A, Gemma A, Kataoka K, Hosoya Y, Noro R, Seike M, Kokubo Y, Watanabe M, Kudoh S.
    • Journal Title

      J Nippon Med Sch. 71(2)

      Pages: 99-104

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] The difference of angiogenesis in human lung adenocarcinoma cell lines with different metastatic potency.2004

    • Author(s)
      Zou D
    • Journal Title

      J Nippon Med Sch. 71(3)

      Pages: 181-189

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Reduced transcription of the RB2/p130 gene in human lung cancer2003

    • Author(s)
      Xue Jun H
    • Journal Title

      Mol Carcinog 38(3)

      Pages: 124-129

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Somatic mutation of the Caspase-5 gene in human lung cancer2003

    • Author(s)
      Hosomi Y
    • Journal Title

      Int J Mol Med 12(4)

      Pages: 443-446

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Proteomic analysis of intestinal epithelial cells expressing stabilized beta-catenin2003

    • Author(s)
      Seike M
    • Journal Title

      Cancer Res 63(15)

      Pages: 4641-4647

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Increased expression of the LGALS3 (galectin 3) gene in human non-small-cell lung cancer2003

    • Author(s)
      Yoshimura A
    • Journal Title

      Genes Chromosomes Cancer 37(2)

      Pages: 159-164

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Reduced transcription of the RB2/p130 gene in human lung cancer.2003

    • Author(s)
      Xue Jun H
    • Journal Title

      Mol Carcinog. 38(3)

      Pages: 124-129

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Somatic mutation of the Caspase-5 gene in human lung cancer.2003

    • Author(s)
      Hosomi Y
    • Journal Title

      Int J Mol Med. 12(4)

      Pages: 443-446

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Proteomic analysis of intestinal epithelial cells expressing stabilized beta-catenin.2003

    • Author(s)
      Seike M
    • Journal Title

      Cancer Res. 63(15)

      Pages: 4641-4647

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Increased expression of the LGALS3 (galectin 3) gene in human non-small-cell lung cancer.2003

    • Author(s)
      Yoshimura A
    • Journal Title

      Genes Chromosomes Cancer. 37(2)

      Pages: 159-164

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Reduction of PTEN protein and loss of epidermal growth factor receptor (EGFR) gene mutation in lung cancer with natural resistance to gefitinib (IRESSA)

    • Author(s)
      Kokubo Y
    • Journal Title

      British J Cancer (In press)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Alterations in Novel Candidate Tumor Suppressor Genes, ING1 and ING2 in Human Lung Cancer

    • Author(s)
      Okano T
    • Journal Title

      Oncology Report (In press)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Reduction of PTEN protein and loss of epidermal growth factor receptor(EGFR) gene mutation in lung cancer with natural resistance to gefitinib(IRESSA).

    • Author(s)
      Kokubo Y
    • Journal Title

      Br J Cancer (in press)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Alterations in Novel Candidate Tumor Suppressor Genes, ING1 and ING2 in Human Lung Cancer.

    • Author(s)
      Okano T
    • Journal Title

      Oncology Report (in press)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Reduction of PTEN protein and loss of epidermal growth factor receptor (EGFR) gene mutation in lung cancer with natural resistance to gefitinib (IRESSA)

    • Author(s)
      Kokubo Y
    • Journal Title

      British J Cancer (In press)

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Alterations in Novel Candidate Tumor Suppressor Genes, ING1 and ING2 in Human Lung Cancer

    • Author(s)
      Okano T
    • Journal Title

      Oncology Report (In press)

    • Related Report
      2004 Annual Research Report
  • [Publications] Xue Iun H: "Reduced transcription of the RB2/p130 gene in human lung cancer."Mol Carcinog.. 38(3). 124-129 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Hosomi Y: "Somatic mutation of the Caspase-5 gene in human Iung cancer."Int J Mol Med.. 12(4). 443-446 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Seike M: "Proteomic analysis of intestinal epithelial cells expressing stabilized beta-catenin."Cancer Res.. 63(15). 4641-4647 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Yoshimura A: "Increased expression of the LGALS3 (galectin 3) gene in human non-small-cell lung cancer."Genes Chromosomes Cancer. 37(2). 159-164 (2003)

    • Related Report
      2003 Annual Research Report

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Published: 2003-04-01   Modified: 2016-04-21  

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