| Project/Area Number |
15590884
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Neurology
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| Research Institution | Shinshu University |
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Principal Investigator |
TIAN Qing Bao Shinshu University, Graduate School of Medicine Department of Neuroplasticity, Assistant Professor, 大学院・医学研究科, 助手 (40324264)
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| Co-Investigator(Kenkyū-buntansha) |
SUZUKI Tatsuo Shinshu University, Graduate School of Medicine Department of Neuroplasticity, Professor, 大学院・医学研究科, 教授 (80162965)
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| Project Period (FY) |
2003 – 2004
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| Project Status |
Completed (Fiscal Year 2004)
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| Budget Amount *help |
¥3,000,000 (Direct Cost: ¥3,000,000)
Fiscal Year 2004: ¥1,100,000 (Direct Cost: ¥1,100,000)
Fiscal Year 2003: ¥1,900,000 (Direct Cost: ¥1,900,000)
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| Keywords | Neurodegeneration / Ubiquitin / Proteasome / Parkinson's Disease / Aggresome / Ubiquitin Specific Protease / Post Synaptic Density / ubiquitin / ubiquitin-specific protease / post-synaptic density / synapse / proteasome / de-ubiquitin / lipid raft |
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| Research Abstract |
Recent reports suggest an important role for protein ubiquitination in synaptic plasticity. We cloned, from the rat brain, a novel gene that encoded an ubiquitin-specific protease (USP), and termed this protein synaptic ubiquitin-specific protease (synUSP, GenBank TM Accession no. AB073880). The homologous human gene was mapped to a locus on chromosome 1p36.12. The deduced synUSP protein consisted of 1036 amino acids, and possessed an ubiquitin-like domain at the C-terminus, Cys-and His-boxes, leuane zipper motifs, and six amino add-repeats of L/ILCPHG. The protein possessed de-ubiquitinating activity toward a model substrate, as expected from its sequence. The protein of 125 kDa was present in the rat brain ; in particular, it was enriched in the post-synaptic density and the dendritic lipid raft fractions. The immunostaining of cortical neurons confirmed the postsynaptic localization. The mRNA for synUSP was localized to dendrites, as well as somas, of neuronal cells. Thus, both the mRNA and the protein were localized in the post-synaptic compartments. These results suggest a regulatory mechanism for the ubiquitin-related system at the post synaptic sites.
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