| Project/Area Number |
15590894
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Neurology
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| Research Institution | KYUSHU UNIVERSITY |
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Principal Investigator |
MURAI Hiroyuki Kyushu University, University Hospital, Assistant professor, 大学病院, 講師 (80325464)
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| Co-Investigator(Kenkyū-buntansha) |
ARAKI Norie Kumamoto University, Faculty of medicine, Assistant professor, 医学部, 講師 (80253722)
IKEZOE Koji Kyushu University, University Hospital, Assistant professor, 大学病院, 講師 (80343317)
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| Project Period (FY) |
2003 – 2004
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| Project Status |
Completed (Fiscal Year 2004)
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| Budget Amount *help |
¥3,500,000 (Direct Cost: ¥3,500,000)
Fiscal Year 2004: ¥1,300,000 (Direct Cost: ¥1,300,000)
Fiscal Year 2003: ¥2,200,000 (Direct Cost: ¥2,200,000)
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| Keywords | multiple sclerosis / autoantibody / axonal injury / two-dimension immunoblot / proteomic analysis |
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| Research Abstract |
Pathologically, brain from multiple sclerosis (MS) patients show demyelination as well as axonal injury. Recently, it is indicated that axonal damage is profoundly related to the residual neurological deficit. Therefore, mechanism of axonal damage in MS has attracted a considerable attention. We applied proteomic analysis to identify the protein that is related to axonal damage of MS. Patients having conventional MS, optico-spinal MS, chronic cortical inflammation were screened for anti-neuronal antibody by single dimension immunoblotting. Patients who showed positive result was appled to two-dimension electrophoresis (2-DE). 2-DE was carried out in a horizontal electrophoresis system, for the first dimensional isoelectric focusing using Immobiline dry strip and by a Giant-slab gel electrophoresis system for the second-dimensional SDS-PAGE. The protein spots on the gels were electroblotted onto a polyvinylidene di£luoride (PVDF) membrane with a semi-dry blotting apparatus. The PVDF membrane was stained with 0.1% Coomassie Brilliant Blue (CBB). The stained gels and PVDF membranes were scanned and analyzed. Mass analysis revealed that one of the spot was fascin, which is a protein which connect axon to the oligodendroglia. This is one of the mechanisms that may playa role in developing axonal injury in MS.
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