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Screening of clinical medicines for effects on gene expressions and protein productions of adipocytokines by using a newly-isolated preadipocyte line

Research Project

Project/Area Number 15590926
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Metabolomics
Research InstitutionIwate Medical University

Principal Investigator

SATOH Jo  Iwate Medical University, School of Medicine, Professor, 医学部, 教授 (60125565)

Co-Investigator(Kenkyū-buntansha) DOI Hideyuki  Tohoku University, Tohoku University Hospital, Assistant Professor, 助教授 (90188839)
TAKAHASHI Kazuma  Tohoku University, Tohoku University Hospital, Assistant Professor, 助手 (60292215)
KANEKO Yoshihito  Iwate Medical University, School of Medicine, Lecturer, 医学部, 講師 (80285585)
ISHIDA Wataru  Iwate Medical University, School of Medicine, Assistant Professor, 医学部, 助手 (80305995)
Project Period (FY) 2003 – 2004
Project Status Completed (Fiscal Year 2004)
Budget Amount *help
¥2,900,000 (Direct Cost: ¥2,900,000)
Fiscal Year 2004: ¥1,400,000 (Direct Cost: ¥1,400,000)
Fiscal Year 2003: ¥1,500,000 (Direct Cost: ¥1,500,000)
KeywordsAdipocytes / AP-18 / Adiponectin / TNF-aloha / Glucose transnorter-4 / Gliclazide / Glibenclamide / Pioglitazone / IL-1β / IL-6 / レプチン / GLUT4 / グリクラシド / デラプリル
Research Abstract

We have shown that the AP-18 cell, an preadipocyte line newly isolated from subcutaneous tissue of the 03H/He mouse, expresses various adipocyte-specific and adipocyte?related genes, and spontaneously differentiates to mature adipocyte during culture with insulin.
Using AP-1 8 cells in vitro, we observed effects of various clinical medicines used for diabetic patients on gene expressions of adiponectin and GLUT4, and on production of adiponectin protein. Gliclazide and glibenclamide (sulfonylureas), pioglitazone (thiazolidinedione), and delapril (ACE inhibitor) increased gene expressions of adiponectin and GLUT4, and adiponectin protein production, whereas metformin (biguanide), temocapril and captopril (ACE inhibitors), candesartane (ARB), doxazosine (alpha-i blocker), metprolol and selectol (beta-1 blockers), and methylcysteine (cysteinderivative) had no such effects.
Because it has been indicated that sulfonylurea stimulates adipocytes to produce adiponectin, we measured serum concentration of adipocytekines including adiponectin, TNF-alpha, IL-i-beta, IL-6 and leptin in patients (n = 14 ? 16) treated with gliclazide or glibenclimide to observe in vivo effects of these medicines. There was a tendency that serum adiponecitn concentration was higher in patients treated with gliclazide than those with glibenclimide, and serum TN F-alpha concentration was vice versa, although sample size was small.
The study using AP-i8 cells indicates that sulfonylurea increases gene expressions of adiponectin and GLUT-4, and production of adiponecitn protein.

Report

(3 results)
  • 2004 Annual Research Report   Final Research Report Summary
  • 2003 Annual Research Report
  • Research Products

    (4 results)

All 2005

All Journal Article (4 results)

  • [Journal Article] Liver fat content measured by magnetic resonance spectroscopy at 3.0-tesla independently correlates with plasminogen activator inhibitor-1 and body mass index in type 2 diabetic subjects.2005

    • Author(s)
      Ishii M, Yoshioka Y, Ishida W, Kaneko Y, Fujiwara F, Taneichi H, Miura M, Toshihiro M, Takebe N, Iwai M, Suzuki K, Satoh I.
    • Journal Title

      Tohoku J Exp Med. 206(1)

      Pages: 23-30

    • NAID

      10015467608

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Low incidence of vascular complications in patients with diabetes mellitus associated with liver cirrhosis as compared with type 2 diabetes mellitus.2005

    • Author(s)
      Fujiwara F, Ishii M, Taneichi H, Miura M, Toshihiro M, Takebe N, Ishida W, Kaneko Y, Kato A, Suzuki K, Satoh J
    • Journal Title

      Tohoku J Exp Med. 205(4)

      Pages: 327-334

    • NAID

      10014481998

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Low incidence of vascular complications in patients with diabetes mellitus associated with liver cirrhosis as corn ared with t e 2 diabetes mellitus.2005

    • Author(s)
      Fujiwara F, Ishil M, Taneichi H, Miura M, Toshihiro M, Takebe N, Ishida W, Kaneko Y, KatoA, Suzuki K, Satoh J
    • Journal Title

      Tohoku J Exp Med. 205(4)

      Pages: 327-334

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Liver fat content measured by magnetic resonance spectroscopy at 3.0-tesla independently correlates with plasminogen activator inhibitor-i and body mass index in type 2 diabetic subjects.2005

    • Author(s)
      lshii M, Yoshioka Y, Ishida W, Kaneko Y, Fujiwara F, Taneichi H, Miura M, Toshihiro M, Takebe N, lwai M, Suzuki K, Satoh J.
    • Journal Title

      Tohoku J Exp Med. 206(1)

      Pages: 23-30

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary

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Published: 2003-04-01   Modified: 2016-04-21  

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