| Project/Area Number |
15590934
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Metabolomics
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| Research Institution | University of Yamanashi |
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Principal Investigator |
ENDO Toyoshi University of Yamanashi, Department of Research Interdisciplinary Graduate School of Medicine and Engineering, Associate Professor, 大学院・医学工学総合研究部, 助教授 (00152017)
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| Co-Investigator(Kenkyū-buntansha) |
KOBAYASHI Teturo University of Yamanashi, Department of Research Interdisciplinary Graduate School of Medicine and Engineering, Professor, 大学院・医学工学総合研究部, 教授 (30113442)
TANAKA Shouichiro University of Yamanashi Hospital, 医学部附属病院, 医員 (70377521)
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| Project Period (FY) |
2003 – 2004
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| Project Status |
Completed (Fiscal Year 2004)
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| Budget Amount *help |
¥3,500,000 (Direct Cost: ¥3,500,000)
Fiscal Year 2004: ¥1,300,000 (Direct Cost: ¥1,300,000)
Fiscal Year 2003: ¥2,200,000 (Direct Cost: ¥2,200,000)
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| Keywords | pancreatic puodenal homeobox-1 / pancreas / β cell / organogenesis / pancreatic duct / 膵臓 / β細胞 / トランスジェニックマウス / 糖尿病 / pancreatic duodenal / homeobox-1 / EYFP |
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| Research Abstract |
Pancreatic duodenal homeobox-1 (PDX-1) is the key molecule in organogenesis of the pancreas. To further investigate its roles in organogenesis of pancreas, we developed PDX-1 promoter/YFP transgenic mouse. We cloned rat PDX-1 promoter region (-6.2 Kb) and then inserted into multiple cloning site of pEYFP. Six lines of mouse were transgened and established. Development and growth of the transgenic animal were normal and blood glucose levels of those were also normal range. Morphologically, islets of those were relatively large, and number of beta cells was increased. However, the glucagons-positive cells formed a cluster and localized near the pancreatic duct, which were also hyperplastic. Focally, lymphocyte infiltration around the ducts was observed. It will be needed to analyze detail the role of the transgened cis element in those phenotypes in pancreas.
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