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Reorganization of actin cytoskeleton by RhoA is necessary for GLUT4 translocation in 3T3L1 adipocytes.

Research Project

Project/Area Number 15590935
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Metabolomics
Research InstitutionShinshu University

Principal Investigator

SHIGEMATSU Satoshi  Shinshu University, School of Medicine, Hospital, assistant, 医学部附属病院, 助手 (50262720)

Co-Investigator(Kenkyū-buntansha) YAMAUCHI Keishi  Shinshu University, School of Medicine, Hospital, lecturer, 医学部附属病院, 講師 (30191191)
Project Period (FY) 2003 – 2005
Project Status Completed (Fiscal Year 2005)
Budget Amount *help
¥3,700,000 (Direct Cost: ¥3,700,000)
Fiscal Year 2005: ¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 2004: ¥800,000 (Direct Cost: ¥800,000)
Fiscal Year 2003: ¥2,400,000 (Direct Cost: ¥2,400,000)
Keywordsinsulin / GLUT4 / cadherin / catenin / Rho / actin / RhoA / p120-カテニン / N-カドヘリン
Research Abstract

p120-catenin is a member of Armadillo-domain proteins found at cell-cell junctions, and interacts with the highly conserved juxtamembrane domain of classical cadherins. There are three kinds of cadherin expressed in 3T3L1 pre-adipocytes, but their expressions are reduced significantly after differentiation to adipocytes. Therefore, main part of p120-catenin goes to cytosole fraction. This unbound form of p120-catenin acts as a Rho-GDI in cytosole, then actin stress fiber disappears in adipocytes since RhoA plays critical role to maintaining actin stress fiber. The other hand, cortical actin, other F-actin structure, is increased in adipocytes. After insulin stimulation, GLUT4 translocation from intracellular GLUT4 storage compartments to plasma membrane occurred simultaneously with up regulation of interaction between cadherin and p120-catenin. Over expression of N-cadherin in adipocytes to suppress Rho-GDI activity of p120-catenin induced GLUT4 translocation to plasma membrane without insulin stimulation. Moreover, a constitutively active RhoA mutant induced GLUT4 translocation and thickened the cortical actin. These results indicated that p120-catenin is a key molecule for regulation of actin structures and GLUT4 translocation, and the functions are controlled through RhoA activity. Taken together, it is possible that one mechanism of GLUT4 translocation by insulin is through p120-catenin, but it is necessary to examine the relationship with other signal cascades of insulin, PKB and ERK pathways, to confirm the importance of the function of p120-catenin.

Report

(4 results)
  • 2005 Annual Research Report   Final Research Report Summary
  • 2004 Annual Research Report
  • 2003 Annual Research Report
  • Research Products

    (6 results)

All 2004 Other

All Journal Article (5 results) Publications (1 results)

  • [Journal Article] Vascular Endothelial cell growth factor attenuates actions of transforming growth factor-beta in human Endothelial cells.2004

    • Author(s)
      Yamauchi K, Nishimura Y, Shigematsu S, et al.
    • Journal Title

      J Biol Chem. 279

      Pages: 55104-8

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Vascular Endothelial cell growth factor attenuates actions of transforming growth factor-beta in human Endothelial cells.2004

    • Author(s)
      Keishi Yamauchi, Yoshihiro Nishimura, Satoshi Shigematsu, Yuichiro Takeuchi, Junko Nakamura, Toru Aizawa, Kiyoshi Hashizume
    • Journal Title

      J.Biol.Chem. 279

      Pages: 55104-55108

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Vascular endothelial cell growth factor attenuates actions of transforming growth factor-beta in human endothelial cells.2004

    • Author(s)
      Yamauchi K, Nishimura Y, Shigematsu S, et al.
    • Journal Title

      J Biol Chem 279

      Pages: 55104-55108

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Dual regulation of Rac and Rho by p120 catenin controls adipocyte plasma membrane trafficking.

    • Author(s)
      Hou JC, Shigematsu S, et al.
    • Journal Title

      J.Biol.Chem (Papers in Press)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Dual regulation of Rac and Rho by p120 catenin controls adipocyte plasma membrane trafficking.

    • Author(s)
      June Chunqiu Hou, Satoshi Shigematsu, Howard C.Crawford, Panos Z.Anastasiadis, Jeffrey E.Pessin.
    • Journal Title

      J.Biol.Chem. (in Press)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Publications] 重松 理: "The Adipocyte Plasma Membrane Caveolin Functional / Structural Organization Is Necessary for the Efficient Endocytosis of GLUT4"the Journal of Biological Chemistry. 278. 10683-10690 (2003)

    • Related Report
      2003 Annual Research Report

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Published: 2003-04-01   Modified: 2016-04-21  

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