| Project/Area Number |
15591011
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Hematology
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| Research Institution | Saga University (2004)
佐賀医科大学 (2003) |
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Principal Investigator |
FUKUDOME Kenji Saga University, Medicine, Associate Professor, 医学部, 助教授 (50284625)
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| Co-Investigator(Kenkyū-buntansha) |
KIMOTO Masao Saga University, Medicine, Professor, 医学部, 教授 (40153225)
TSUNEYOSHI Naoko Saga University, Medicine, Assistant Professor, 医学部, 助手 (80336114)
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| Project Period (FY) |
2003 – 2004
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| Project Status |
Completed (Fiscal Year 2004)
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| Budget Amount *help |
¥3,500,000 (Direct Cost: ¥3,500,000)
Fiscal Year 2004: ¥1,300,000 (Direct Cost: ¥1,300,000)
Fiscal Year 2003: ¥2,200,000 (Direct Cost: ¥2,200,000)
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| Keywords | thrombosis / endothelial / monoclonal antibody / blood coagulation |
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| Research Abstract |
To investigate the molecular mechanism for regulation of blood coagulation, we prepared functional blocking monoclonal antibodies(MAbs) against blood coagulation factors and the relation factors. We established function-blocking MAbs against the endothelial cell protein C receptor(EPCR). As shown in the references, we could demonstrate novel physiological function of EPCR. To collaborate with the thrombin receptor, EPCR mediated signal trunsduction triggered by activated protein C(APC). The signal resulted anti-inflammatory and anti-apoptotic responses in endothelial cells. We also established MAbs against to thrombomodulain, which reacted to each domains in the extracellular region. Analysis with the antibodies demonstrated possibilities of unknown molecular mechanism for protein C activation. The detail analysis for the mechanism is underway. MAbs against mouse prothrombin and antithrombin III were also established. We are trying to establish a sandwich ELISA system for detection of thrombin/ATIII complex in mice. By using these materials, we would like to investigate the molecular mechanism of in vivo regulation of blood coagulation.
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