| Project/Area Number |
15591059
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (C)
|
| Allocation Type | Single-year Grants |
|---|
| Section | 一般 |
|---|
| Research Field |
膠原病・アレルギー・感染症内科学
|
|---|
| Research Institution | Osaka University |
|---|
Principal Investigator |
TOMONO Kazunori Osaka University, Graduate School of Medicine, Assistant Professor, 医学系研究科, 助教授 (40202204)
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
KOHNO Shigeru Nagasaki University, Graduate School of Biomedical, Professor, 医薬総合研究科, 教授 (80136647)
|
|---|
| Project Period (FY) |
2003 – 2004
|
| Project Status |
Completed (Fiscal Year 2004)
|
| Budget Amount *help |
¥3,100,000 (Direct Cost: ¥3,100,000)
Fiscal Year 2004: ¥1,300,000 (Direct Cost: ¥1,300,000)
Fiscal Year 2003: ¥1,800,000 (Direct Cost: ¥1,800,000)
|
|---|
| Keywords | respiratory infection / Pseudomonas aeruginosa / mucosal biofilm / bacterial colonization / Quorum sensing / in vivo model / 抗菌薬感受性 / 慢性気道感染症 / 実験モデル / 慢性細菌性感染症 / 抗菌化学療法 / マクロライド |
|---|
| Research Abstract |
We studied the role of quorum sensing system in a mouse model of chronic Pseudomonas aeruginosa infection. A chronic P.aeruginosa respiratory infection model was established by placement of a tube precoated with PAO1 (wild type), quorum sensing mutants PAO-JP1(Δlasl), PDO100(Δrhll) or PAO-JP2(Δlasl/Δrhll), in the bronchus. At day 14 after infection, the numbers of viable bacteria in the quorum sensing mutant groups were lower than in wild type group. Histopathological examination showed milder inflammatory changes in the lung of the mutant groups compared with the wild type group. The percentages of neutrophils in the bronchoalveolar lavage fluid in the quorum sensing system mutant groups were lower than in wild type group. Our findings indicate that the quorum sensing system plays an important role in chronic P.aeruginosa respiratory infection.
|
